Annals of Microbiology

, Volume 64, Issue 2, pp 431–439 | Cite as

Enhancement of ABE fermentation through regulation of ammonium acetate and D–xylose uptake from acid-pretreated corncobs

  • Akarin Boonsombuti
  • Kittinan Komolpis
  • Apanee Luengnaruemitchai
  • Sujitra Wongkasemjit
Original Article
First Online:
Received:
Accepted:

Abstract

Clostridium acetobutylicum TISTR 1462 and Clostridium beijerinckii TISTR 1461 were chosen to optimize acetone–butanol–ethanol (ABE) fermentation by using glucose as a carbon source. The enhancement in its productivity by adding various concentrations of ammonium acetate was studied. Then, the variation of glucose/xylose ratios in the pre-grown medium was investigated. The results showed that both increased ammonium acetate in the production medium and D–xylose in the pre-grown medium could produce more ABE. With these conditions, using corncob hydrolysate as a substrate, 20.58 g/L ABE was produced from C. beijerinckii TISTR 1461 with 0.44 g/L/h and 0.45 of ABE productivity and yield, respectively.

Keywords

Butanol Clostridium acetobutylicum Clostridium beijerinckii Corncobs Fermentation 
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Notes

Acknowledgments

We are grateful to the National Research University Project of CHE and the Ratchadaphiseksomphot Endowment Fund (EN269B), the Development and Promotion of Science and Technology Talents Project (DPST), and the Center of Excellence for Petrochemical and Materials Technology, Thailand, for supporting this research. The authors would also like to thank Betagro Public Company Limited for providing corncob samples used in this research. In addition, the authors thank Dr. Charnwit Kositanont for his valuable suggestions.

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Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2013

Authors and Affiliations

  • Akarin Boonsombuti
    • 1
  • Kittinan Komolpis
    • 2
  • Apanee Luengnaruemitchai
    • 1
    • 3
  • Sujitra Wongkasemjit
    • 1
    • 3
  1. 1.The Petroleum and Petrochemical CollegeChulalongkorn UniversityBangkokThailand
  2. 2.The Institute of Biotechnology and Genetic EngineeringChulalongkorn UniversityBangkokThailand
  3. 3.Center of Excellence on Petrochemical and Materials TechnologyChulalongkorn UniversityBangkokThailand

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