Diversity of cultivable bacteria associated with fruiting bodies of wild Himalayan Cantharellus spp.
- 334 Downloads
The cultivable bacteria associated with fruiting bodies of different Cantharellus species collected from the forests of the north-western Himalayan region were studied. Repetitive extragenic palindromic (REP) and BOX-PCR fingerprinting analyses revealed that different strains were distributed within Cantharellus species. The number of bacteria varied from 0.5 to 1.1 × 103 CFU/g of fresh tissue for different fruiting bodies. Thirty different operational taxonomic units (OTUs) were found among the bacteria analyzed. 16S rRNA sequence analysis revealed that most of the bacteria associated with different Cantharellus species were Gammaproteobacteria belong to the genera Hafnia, Enterobacter, Ewingella, Rahnella, Stenotrophomonas, and Pseudomonas, and γ-proteobacterium, followed by Betaproteobacteria (Alcaligenes) and Firmicutes, (Bacillus). The most common bacteria associated with the majority of Cantharellus species were Hafnia and Stenotrophomonas species.
KeywordsEndophytic bacteria Mycorrhization helper bacteria Hafnia sp. Stenotrophomonas sp. Golden chanterelle
The authors are grateful to TIFAC-CORE, Thapar University, Patiala for the facilities.
- Barbieri E, Bertini L, Rossi I, Ceccaroli P, Saltarelli R, Guidi C, Zambonelli A, Stocchi V (2005) New evidence for bacterial diversity in the ascoma of the ectomycorrhizal fungus Tuber borchii Vittad. FEMS Microbiol Lett 247 :23–35. Google Scholar
- Dahm H, Wrótniak W, Strzelczyk E, Li C-Y, Bednarska E (2005) Diversity of culturable bacteria associated with fruiting bodies of ectomycorrhizal fungi. Phytopathol Polon 38:51–62Google Scholar
- Gazzanelli G, Malatesta M, Pianetti A, Baffone W, Stocchi V, Citterio B (1999) Bacteria associated to fruit bodies of ectomycorrhizal fungus Tuber borchii Vittad. Symbiosis 26:211–222Google Scholar
- Kreig NR, Holt JG (1984) Bergey’s Manual of Systematic Bacteriology, vol. I. Williams and Wilkins, BaltimoreGoogle Scholar
- Miethling R, Wieland G, Backhaus H, Tebbe CC (2000) Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L33. Microbial Ecol 40:43–56Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
- Timonen S, Jørgensen KS, Haahtela K, Sen R (1998) Bacterial community structure at defined locations of Pinus sylvestris–Suillus bovinus and Pinus sylvestris–Paxillus involutus mycorrhizospheres in dry pine forest humus and nursery peat. Can J Microbiol 44:499–513Google Scholar
- Varese GC, Trotta PA, Scannerini S, Luppi-Mosca AM, Martinotti G (1996) Bacteria associated with Suillus grevillei sporocarps and ectomycorrhizae and their effects on in vitro growth of the mycobiont. Symbiosis 21:129–147Google Scholar
- Versalovic J, Schneider M, de Bruijn FJ, Lupski JR (1994) Genomic fingerprinting of bacteria using repetitive sequence-based Polymerase Chain Reaction. Methods Mol Cell Biol 5:25–40Google Scholar