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Comparing submerged and solid-state fermentation of agro-industrial residues for the production and characterization of lipase by Trichoderma harzianum

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Abstract

Lipase production by Trichoderma harzianum was evaluated in submerged fermentation (SF) and solid-state fermentation (SSF) using a variety of agro-industrial residues. Cultures in SF showed the highest activity (1.4 U/mL) in medium containing 0.5 % (w/v) yeast extract, 1 % (v/v) olive oil and 2.5 C:N ratio. This paper is the first to report lipase production by T. harzianum in SSF. A 1:2 mixture of castor oil cake and sugarcane bagasse supplemented with 1 % (v/w) olive oil showed the best results among the cultures in SSF (4 U/g ds). Lipolytic activity was stable in a slightly acidic to neutral pH, maintaining 50 % activity after 30 min at 50 °C. Eighty percent of the activity remained after 1 h in 25 % (v/v) methanol, ethanol, isopropanol or acetone. Activity was observed with vegetable oils (olive, soybean, corn and sunflower) and long-chain triacylglycerols (triolein), confirming the presence of a true lipase. The results of this study are promising because they demonstrate an enzyme with interesting properties for application in catalysis produced by fermentation at low cost.

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Acknowledgments

The authors acknowledge financial support from Foundation for the Development of UNESP (FUNDUNESP) and Foundation for Research Support of São Paulo State (FAPESP), Brazil.

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Authors and Affiliations

  1. Laboratório de Biotecnologia Industrial, Departamento de Ciências Biológicas, Universidade Estadual Paulista (UNESP), Campus de Assis, Assis, SP, Brazil

    Gilberto Victor Coradi, Viviane Loiola da Visitação, Evandro Antônio de Lima, Larissa Yumi Tsuchida Saito, Darío Abel Palmieri, Pedro de Oliva Neto & Valéria Marta Gomes de Lima

  2. Centro de Citricultura, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil

    Marco Aurélio Takita

Authors
  1. Gilberto Victor Coradi
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  2. Viviane Loiola da Visitação
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  3. Evandro Antônio de Lima
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  4. Larissa Yumi Tsuchida Saito
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  5. Darío Abel Palmieri
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  6. Marco Aurélio Takita
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  7. Pedro de Oliva Neto
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  8. Valéria Marta Gomes de Lima
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Correspondence to Valéria Marta Gomes de Lima.

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Coradi, G.V., da Visitação, V.L., de Lima, E.A. et al. Comparing submerged and solid-state fermentation of agro-industrial residues for the production and characterization of lipase by Trichoderma harzianum . Ann Microbiol 63, 533–540 (2013). https://doi.org/10.1007/s13213-012-0500-1

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  • DOI: https://doi.org/10.1007/s13213-012-0500-1

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