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Annals of Microbiology

, Volume 62, Issue 4, pp 1523–1530 | Cite as

In vitro antibiofilm activity of the melanin from Auricularia auricula, an edible jelly mushroom

  • Li Bin
  • Li Wei
  • Chen Xiaohong
  • Jiang Mei
  • Dong MingshengEmail author
Original Article

Abstract

A melanin derived from Auricularia auricula was characterized based on ultraviolet-visible (UV), infrared (IR), electron paramagnetic resonance (EPR) spectra and chemical tests. The antibiofilm activity of the isolated A. auricula melanin against Escherichia coli K-12, Pseudomonas aeruginosa PAO1 and P. fluorescens P-3 was examined using crystal violet and LIVE/DEAD BacLight staining as well as confocal laser scanning microscopy (CLSM). The results showed that A. auricula melanin could inhibit significantly biofilm formation of E. coli K-12, P. aeruginosa PAO1 and P. fluorescens P-3, while no inhibitory effects on growth were found. CLSM analysis showed that A. auricula melanin treatment resulted in thinner and looser biofilm compared with the control. The current study reveals for the first time the antibiofilm activity of melanin derived from A. auricula.

Keywords

Auricularia auricula Melanin Antibiofilm Escherichia coli Pseudomonas aeruginosa Pseudomonas fluorescens 

Notes

Acknowledgments

This work was supported by Natural Science Foundation of China (No.30700627) and Agricultural Science Independent Innovation Fund of Jiangsu [No.CX (08) 113].

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Copyright information

© Springer-Verlag and the University of Milan 2012

Authors and Affiliations

  • Li Bin
    • 1
  • Li Wei
    • 1
  • Chen Xiaohong
    • 1
  • Jiang Mei
    • 1
  • Dong Mingsheng
    • 1
    Email author
  1. 1.College of Food Science and TechnologyNanjing Agricultural UniversityNanjingPeople’s Republic of China

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