Abstract
The aim of the current study was to apply a mixed culture of Candida utilis and Brevibacterium lactofermentum instead of using a mono-culture of B. lactofermentum or yeast to produce higher amount of amino acids, crude protein and true protein by using the maximum amount of substrate mixture, which B. lactofermentum alone cannot utilize. This mixed culture offered a combination that yielded high crude protein content and converted the substrate mixture efficiently into microbial biomass protein. Best results were obtained with sequential fermentation carried out with B. lactofermentum added after 3 days to C. utilis culture grown on beet pulp hydrolysate supplemented with molasses and glucose. Mixed culture of C. utilis and B. lactofermentum maintained the properties of each individual fermentation, with high production of microbial biomass protein in optimized medium. Crude protein increased from 11.3% to 54.5% and dry cell mass to 48 g/L with mixed culture. The amino acid profile of the final microbial biomass protein obtained by mixed culture of C. utilis and B. lactofermentum in a 75-L fermentor remained unchanged, and was enriched with all essential and non-essential amino acids. Mixed culture in this study thus exhibits a synergistic effect with possible industrial application.
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References
Ahmed S, Qurrat-ul-Ain, Aslam N, Naeem S, Sajjad-ur-Rehman, Jamil A (2003) Induction of xylanase and cellulase genes from Trichoderma harzianum with different carbon sources. Pak J Biol Sci 6:1912–1916
Ahmed S, Aslam N, Latif F, Rajoka MI, Jamil A (2005) Molecular cloning of cellulase genes from Trichoderma harzianum. In: Atta-ur-Rehman, Choudhary, Khan (eds) Frontiers in natural product chemistry, vol 1. Bentham, The Netherlands, pp 73–75.
Ahmed S, Jabeen A, Jamil A (2007) Xylanase from Trichoderma harzianum: enzyme characterization and gene isolation. J Chem Soc Pak 29:176–182
Ahmed S, Riaz S, Jamil A (2009a) Molecular cloning of fungal xylanases: an overview. Appl Microbiol Biotechnol 84:19–35
Ahmed S, Bashir A, Saleem H, Saadia M, Jamil A (2009b) Production and purification of cellulose-degrading enzymes from a filamentous fungus Trichoderma harzianum. Pak J Bot 41:1411–1419
Ahmed S, Ahmad F, Hashmi AS (2010) Production of microbial biomass protein by sequential culture fermentation of Arachniotus sp. and Candida utilis. Pak J Bot 42:1225–1234
Aiba S, Humphrey AE, Millis NF (1973) Biochemical engineering, 2nd edn. Academic, New York, pp 92–127
Ali S, Ahmed S, Sheikh MA, Hashmi AS, Rajoka MI, Jamil A (2009) Lysine production by l-homoserine resistant mutant of Brevibacterium flavum. J Chem Soc Pak 31:97–102
Association of Official Analytical Chemists (1984). Official methods of analysis, 14th edn. Washington, DC. ISBN 0-93558424-2
Athar M, Ahmed S, Hashmi AS (2009) Bioconversion of beet pulp to microbial biomass protein by Candida utilis. J Chem Soc Pak 31:115–121
Ekwealor IA, Obeta JAN (2005) Studies on lysine production by Bacillus megaterium. Afr J Biotechnol 4:633–638
Fonseca GG, Heinzle E, Wittmann S, Gombert AK (2008) The yeast Kluyveromyces marxianus and its biotechnological potential. Appl Microbiol Biotechnol 79:339–354
Ghanem KM (1992) Single-cell protein production from beet pulp by mixed culture. Microbiologia 8:39–43
Ghori MI, Ahmed S, Malana MA, Jamil A (2011) Corn stover-enhanced cellulase production by Aspergillus niger NRRL 567. Afr J Biotechnol 10(31):5878–5886
Irshad M, Ahmed S, Latif F, Rajoka MI (2008) Regulation of endo-β-d-Xylanase and β-xylosidase synthesis in Humicola lanuginosa. J Chem Soc Pak 30:913–918
Jamil A, Naim S, Ahmed S, Ashraf M (2005) Production of industrially important enzymes using molecular approaches; cellulases and xylanases. In: Thangadurai D, Pullaiah T, Balatti PA (eds) Genetic resources and Biotechnology II, vol 2. Regency, New Delhi
Jin B, Yu Q, van Leeuwen JH (2001) A bioprocessing mode for simultaneous fungal biomass protein production and wastewater treatment using an external air-lift bioreactor. J Chem Technol Biotechnol 76:1041–1048
Paul D, Mukhopadhyay R, Chatterjee BP, Guha AK (2002) Nutritional profile of food yeast Kluyveromyces fragilis biomass grown on whey. Appl Biochem Biotechnol 97:209–218
Rajoka MI, Khan S, Jabbar MA, Awan MS, Hashmi AS (2006) Kinetics of batch single cell protein production from rice-polishings with Candida utilis in continuously aerated tank reactors. Bioresour Technol 97:1934–1941
Saadia M, Ahmed S, Jamil A (2008) Isolation and cloning of cre1 gene from a filamentous fungus Trichoderma harzianum. Pak J Bot 40:421–426
Saleem F, Ahmed S, Jamil A (2008) Isolation of a xylan degrading gene from genomic DNA library of a thermophilic fungus Chaetomium thermophile ATCC 28076. Pak J Bot 40:1225–1230
Sattar M, Ahmed S, Sheikh MA, Hashmi AS (2008) Fermentation of yeast sludge with Brevibacterium flavum to enhance lysine concentration. J Chem Soc Pak 30:642–648
Sheikh MA, Aslam N, Ahmed S, Latif F, Rajoka MI, Jamil A (2003) Isolation and cloning of xylanase and beta-glucosidase genes from Trichoderma harzianum. Mol Cell Proteomics 2(9):866
Shiratsuchi M, Kuronuma H, Kawahara Y, Yoshihara Y, Miwa H, Nakamori S (1995) Simultaneous and high fermentative production of l-lysine and l-glutamic acid using a strain of Brevibacterium lactofermentum. Biosci Biotechnol Biochem 59:83–86
Singh A, Abidi AB, Agrawal AK, Darmwal NS (1991) Single cell protein production by Aspergillus niger and its evaluation. Zentralbl Mikrobiol 146:181–184
Taherzadeh MJ, Fox M, Hjorth H, Edebo L (2003) Production of mycelium biomass and ethanol from paper pulp sulfite liquor by Rhizopus oryzae. Bioresour Technol 88:167–177
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This research was funded by the University of Agriculture, Faisalabad-38040, Pakistan and the National Institute for Biotechnology and Genetic Engineering, P.O. Box 577 Faisalabad, Pakistan.
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Rajoka, M.I., Ahmed, S., Hashmi, A.S. et al. Production of microbial biomass protein from mixed substrates by sequential culture fermentation of Candida utilis and Brevibacterium lactofermentum . Ann Microbiol 62, 1173–1179 (2012). https://doi.org/10.1007/s13213-011-0357-8
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DOI: https://doi.org/10.1007/s13213-011-0357-8