Annals of Microbiology

, Volume 60, Issue 3, pp 489–494

Antibiotic resistance of Escherichia coli O157:H7 isolated from cattle and sheep

  • Muammer Goncuoglu
  • Fatma Seda Bilir Ormanci
  • Naim Deniz Ayaz
  • Irfan Erol
Original papers


A total of 102 Escherichia coli O157:H7 colonies recovered from 11 cattle and 14 sheep were collected and tested for their antibiotic resistance profiles using a disc diffusion method, according to the Clinical and Laboratory Standards Institute. Four (36.36 %) of the 11 cattle E. coli O157:H7 isolates were resistant to cephalothin, one (9.09 %) isolate was resistant to streptomycin, and one (9.09 %) to nalidixic acid. Two (14.28 %) of the 14 sheep E. coli O157:H7 isolates were resistant to sulphamethoxazole, one (7.14 %) isolate was resistant to sulphonamide compounds, and one (7.14 %) to streptomycin. All cattle and sheep isolates were found to be susceptible to cephazolin, gentamicin, ciprofloxacin, imipenem, trimethoprim/ sulphamethoxazole, chloramphenicol, trimethoprim, and ceftiofur. Six cattle isolates were susceptible at a ratio of 54.54 %, and 11 (78.57 %) isolates from sheep were susceptible to all 20 antibiotics tested. As an overall result, 68 % of the E. coli O157:H7 isolates belonging to cattle and sheep were susceptible to all antibiotics tested. On the other hand, most of the E. coli O157:H7 isolates were intermediately resistant to streptomycin, cephalothin, sulphamethoxazole, ampicillin, and kanamycin.


E. coli O157:H7 Antibiotic resistance Cattle Sheep 


  1. Anon (2006). Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement. M100-S16 Vol.26 No.3.Google Scholar
  2. Beutin L, Geier D, Steinruck H, Zimmermann S, Scheuts F (1993) Prevalence and some properties of verotoxin (shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. J Clin Microbiol 31:2483–2488PubMedGoogle Scholar
  3. Byrne C, Erol I, Call J, Buege D, Kaspar CW, Hiemke C, Fedorka-Cray P, Hermosillo J, Ball T, Wallace M, Handy M, Luchansky JB (2003) Characterization of Escherichia coli O157:H7 from downer and healthy dairy cattle in the upper Midwest region of the United States. Appl Environ Microbiol 69:4683–4688CrossRefPubMedGoogle Scholar
  4. Fratamico PM, Bagi LK, Pepe T (2000) A multiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157:H7 in foods and bovine feces. J Food Prot 63:1032–1037PubMedGoogle Scholar
  5. Fukushima H, Hashizume T, Morita Y, Tanaka J, Azuma K, Mizumoto Y, Kaneno M, Matsuura M, Konma K, Kitani T (1999) Clinical experiences in Sakai City Hospital during the massive outbreak of enterohemorrhagic Escherichia coli O157 infections in Sakai City. Pediatr Int 41:213–217CrossRefPubMedGoogle Scholar
  6. Galland JC, Hyatt DR, Crupper SS, Acheson DW (2001) Prevalence, antibiotic susceptibility and diversity of Escherichia coli O157:H7 isolates from a longitudinal study of beef cattle feedlots. Appl Environ Microbiol 67:1619–1627CrossRefPubMedGoogle Scholar
  7. Goncuoglu M, Erol I, Ayaz ND, Bilir Ormanci FS, Kaspar CW (2010) Isolation and genomic characterization of Escherichia coli O157:H7 in bile of cattle. Ann Microbiol 60:293–297Google Scholar
  8. Ikeda K, Ida O, Kimoto K, Takatorige T, Nakanishi N, Tatara K (1999) Effect of early fosfomycin treatment on prevention of hemolytic uremic syndrome accompanying Escherichia coli O157:H7 infection. Clin Nephrol 52:357–362PubMedGoogle Scholar
  9. Khan A, Das SC, Ramamurthy T, Sikdar A, Khanam J, Yamasaki S, Takeda Y, Nair GB (2002) Antibiotic resistance, virulence gene, and molecular profiles of shiga toxin-producing Escherichia coli isolates from diverse sources in Calcutta. India J Clin Microbiol 40:2009–2015Google Scholar
  10. Kim H, Samadpour M, Grimm L, Clausen C, Besser T, Baylor M, Kobayashi J, Neill ML, Schoenknecht F, Tarr P (1994) Characteristics of antibiotic-resistance Escherichia coli O157:H7 in Washington State, 1984–1991. J Infect Dis 170:1606–1609PubMedGoogle Scholar
  11. Kudva IT, Hatfield PG, Hovde CJ (1996) Escherichia coli O157:H7 in microbial flora of sheep. J Clin Microbiol 34:431–433PubMedGoogle Scholar
  12. Kudva IT, Hatfield PG, Hovde CJ (1997) Characterization of Escherichia coli O157:H7 and other shiga-toxin producing E. coli serotypes isolated from sheep. J Clin Microbiol 35:892–899PubMedGoogle Scholar
  13. Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM, Tauxe RV (1999) Food-related illness and death in the United States. Emerg Infect Dis 5:607–625CrossRefPubMedGoogle Scholar
  14. Meng J, Zhao S, Doyle MP, Joseph SW (1998) Antibiotic resistance of Escherichia coli O157:H7 and O157:NM isolated from animals, food, and humans. J Food Prot 61:1511–514PubMedGoogle Scholar
  15. Meng J, Doyle MP, Zhao T, Zhao S (2001) Enterohemorrhagic Escherichia coli O157:H7. In: Doyle MP, Beuchat LR, Montville TJ (eds) Food microbiology: fundamentals and frontiers, 2nd edn. ASM Press, Washington, D.C., pp 193–213Google Scholar
  16. Paiba GA, Gibbens JC, Pascoe SJS, Wilesmith JW, Kidd SA, Byrne C, Ryan JBM, Smith RP, McLaren IM, Futter RJ, Kay ACS, Jones YE, Chappel SA, Willshaw GA, Cheasty T (2002) Faecal carriage of verocytotoxin-producing Escherichia coli O157:H7 in cattle and sheep at slaughter in Great Britain. Vet Rec 150:593–598CrossRefPubMedGoogle Scholar
  17. Scott L, McGee P, Minihan D, Sheridan JJ, Earley B, Leonard N (2006) The characterization of E. coli O157:H7 isolates from cattle faeces and feedlot environment using PFGE. Vet Microbiol 114:331–336CrossRefPubMedGoogle Scholar
  18. Schroeder CM, Zhao C, DebRoy C, Torcolini J, Zhao S, White DG, Wagner DD, McDermott PF, Walker RD, Meng J (2002) Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine and food. Appl Environ Microbiol 68:576–581CrossRefPubMedGoogle Scholar
  19. Schroeder CM, White DG, Meng J (2004) Retail meat and poultry as a reservoir of antimicrobial-resistant Escherichia coli. Food Microbiol 21:249–255CrossRefGoogle Scholar
  20. Shere JA, Barlett KJ, Kaspar CW (1998) Longitudinal study of Escherichia coli O157:H7 dissemination on four dairy farms in Wisconsin. Appl Environ Microbiol 64:1390–1399PubMedGoogle Scholar
  21. Takahashi K, Narita K, Kato Y, Sugiyama T, Koide N, Yoshida T, Yokochi T (1997) Low-level release of Shiga-like toxin (Verocytotoxin) and endotoxin from enterohemorrhagic E. coli treated with imipenem. Antimicrob Agents Chemother 41:2295–2296PubMedGoogle Scholar
  22. Vali L, Wisely KA, Pearce MC, Turner EJ, Knight HI, Smith AW, Amyes SGB (2004) High-level genotypic variation and antibiotic sensitivity among Escherichia coli O157 strains isolated from two Scottish beef cattle farms. Appl Environ Microbiol 70:5947–5954CrossRefPubMedGoogle Scholar
  23. Walsh C, Duffy G, Mahony RO, Fanning S, Blair IS, McDowell DA (2006) Antimicrobial resistance in Irish isolates of verocytotoxigenic Escherichia coli (E. coli)-VTEC. Int J Food Microbiol 109:173–178CrossRefPubMedGoogle Scholar
  24. Wilkerson C, Samadpour M, van Kirk N, Roberts MC (2004) Antibiotic resistance and distribution of tetracycline resistance genes in Escherichia coli O157:H7 isolates from humans and bovines. Antimicrob Agents Chemother 48:1066–1067CrossRefPubMedGoogle Scholar
  25. Wong CS, Jelacic S, Habeeb RL, Watkins SL, Tarr PI (2000) The risk of hemolytic-uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 infections. N Eng J Med 342:1930–1936CrossRefGoogle Scholar
  26. Zhao S, White DG, Ge B, Ayers S, Freidman S, English L, Wagner D, Gaines S, Meng J (2001) Identification and characterization of integron-mediated antibiotic resistance among shiga toxin-producing Escherichia coli isolates. Appl Environ Microbiol 67:1558–1564CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag and the University of Milan 2010

Authors and Affiliations

  • Muammer Goncuoglu
    • 1
  • Fatma Seda Bilir Ormanci
    • 1
  • Naim Deniz Ayaz
    • 1
  • Irfan Erol
    • 1
  1. 1.Department of Food Hygiene and TechnologyAnkara University, School of Veterinary MedicineAnkaraTurkey

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