Annals of Microbiology

, Volume 60, Issue 1, pp 157–160 | Cite as

Resuscitation of 20-year starved Salmonella in seawater and soil

Original Article

Abstract

Salmonella enterica serovar Typhimurium was stressed by incubation in seawater and soil microcosms for 20 years. Recovery of viable but nonculturable Salmonella was obtained by incubation in nutrient broth and buffered peptone water medium for 2 months, and also in a mouse model when administered orally but not by intra-peritoneal injection. The 16S rDNA gene sequences from 20-year stressed strains were homologous, with a high degree of similarity, and all were identical to the respective initial strain.

Keywords

Salmonella Seawater Stress Soil Viable but nonculturable Resuscitation 

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Copyright information

© Springer-Verlag and the University of Milan 2010

Authors and Affiliations

  • Amel Dhiaf
    • 1
    • 2
  • Fethi Ben Abdallah
    • 1
  • Amina Bakhrouf
    • 1
  1. 1.Laboratoire d’Analyse, Faculté de PharmacieTraitement et Valorisation des Polluants de l’Environnement et des ProduitsMonastirTunisie
  2. 2.Cabinet Dr AllayaKsour EssefTunisia

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