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Microfluidic Fabrication of Encoded Hydrogel Microparticles for Application in Multiplex Immunoassay

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Abstract

Recent interests in comprehensive protein surveys and protein biomarker studies have led to an increased demand for simultaneous measurement of multiple proteins in a single sample. Among various multiplex techniques, bead-based immunoassays, which use encoded particles attached with capture probes, have demonstrated distinct advantages of fluid-phase kinetics, high precision, and flexible target selection. In particular, encoded hydrogel particles composed of porous, hydrophilic, three-dimensional polymers have received positive attention because they enhance the binding kinetics of proteins, reduce protein denaturation, and increase the loading density of capture probes. Microfluidic techniques have been extensively used to fabricate the encoded hydrogel particles for multiplex immunoassays, enabling mass-production of highly monodisperse particles with complex morphologies in mild synthesis conditions. In this paper, we review microfluidic techniques available for the synthesis of encoded hydrogel particles and the important design parameters that determine the particles’ immunoassay performance. We also discuss currently reported multiplex immunoassay platforms that are based on encoded hydrogel particles.

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Acknowledgments

This work was supported by the Engineering Research Center of Excellence Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2016R1A5A1010148) and the Next-Generation Biogreen 21 Program funded by Rural Development Administration of Korea (No. PJ013158). This research was also supported by the Basic Science Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018R1D1A1B070465 77 and NRF-2017R1D1A1B03029883).

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  1. Department of Chemical and Biological Engineering, Korea University, Seoul, Korea

    Yoon Ho Roh, Hyun Jee Lee & Ki Wan Bong

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  1. Yoon Ho Roh
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Correspondence to Ki Wan Bong.

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Roh, Y.H., Lee, H.J. & Bong, K.W. Microfluidic Fabrication of Encoded Hydrogel Microparticles for Application in Multiplex Immunoassay. BioChip J 13, 64–81 (2019). https://doi.org/10.1007/s13206-019-3104-z

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