Microfluidic Devices for Eye Irritation Tests of Cosmetics and Cosmetic Ingredients
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In this paper, we describe the development and application of a simple microfluidic device for in vitro irritation tests of cosmetics. The device was fabricated with a three-compartment diffusion system to mimic a hen’s egg test-chorioallantoic membrane (HET-CAM) system. Human umbilical vein endothelial cells were cultured in the three compartments, and the tested substances were injected into the compartment, and then were drawn through the small microfluidic channels. The mortalities of cells in each compartment were monitored using a fluorescent microscope. The TS (toxicity score) values were evaluated on the basis of the mortalities of cells as a function of time when the cells were exposed to the test substances. Four kinds of cosmetic materials were tested with the microfluidic system, and the results were compared with IS (irritation score) values of a HET-CAM test to validate the in vitro irritation test system. The method developed in this study could be used for evaluation of the ocular toxicity of cosmetic ingredients, and could be widely used as an alternative test for cosmetic and medical tests or for the development of new medicines.
KeywordsMicrofludic device HUVEC HET-CAM Eye irritation Alternative test method
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