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BioChip Journal

, Volume 7, Issue 3, pp 201–209 | Cite as

Evaluation of immunoreactivity of in vitro and in vivo models against bacterial synthesized cellulose to be used as a prosthetic biomaterial

  • Gun-Dong Kim
  • Hana Yang
  • Hye Rim Park
  • Cheung-Seog Park
  • Yong Seek ParkEmail author
  • Seung Eun LeeEmail author
Original Article

Abstract

Prosthetic biomaterials are required to be non-toxic, non-thrombogenic, and non-immunogenic. Bacterial cellulose (BC) synthesized by Gluconacetobacter xylinus has recently been studied as a biocompatible material due to its unique features such as high purity, crystallinity, biodegradability, and tensile strength as compared to plant cellulose. Although BC has high potential to be used as biomaterial, its toxicity and immunoreactivity have not been properly studied yet. In this report, we investigated the immunoreactivity of BC in vitro in human umbilical vein endothelial cells (HUVECs) and in vivo using BALB/c mice. We report that BC does not induce apoptosis and necrosis in HUVECs and does not stimulate immune response in both HUVECs and BALB/c mice models. These results suggest that BC may be widely used as a biocompatible biomaterial for tissue engineering and biosensors.

Keywords

Gluconacetobacter xylinus Bacterial cellulose (BC) Biomaterials Immunoreactivity Human umbilical vein endothelial cells (HUVECs) Lipopolysaccharide (LPS) 

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Copyright information

© The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Department of Microbiology, School of MedicineKyung Hee UniversityHoegi-dong, Dongdaemun-gu, SeoulKorea

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