BioChip Journal

, Volume 7, Issue 2, pp 188–193 | Cite as

Aqueous extracts of Anemarrhena asphodeloides stimulate glucagon-like pepetide-1 secretion in enteroendocrine NCI-H716 cells

  • Kang-Hoon Kim
  • Ki-Suk Kim
  • Min Hee Shin
  • Eun Gyeong Jang
  • Eun Young Kim
  • Jang-Hoon Lee
  • Kwang Seok Ahn
  • Jae-Young Um
  • Hyeung-Jin Jang
Original Article
First Online:
Received:
Accepted:

Abstract

Anemarrhena asphodeloides (AA), a bitter taste herbal medicine, has been prescribed in traditional oriental medicine to treat diabetes mellitus. Here, AA was extracted and fractionated to investigate its effects on the stimulation of glucagon-like peptide-1 (GLP-1) secretion in enteroendocrine cells. GLP-1 is secreted from the human enteroendocrine L cells to the blood in response to ingested nutrients. Because GLP-1 increases glucose dependent insulin release, it is known as a therapeutic method for the treatment of type II diabetes mellitus. The human enteroendocrine L cell line NCI-H716 expresses various chemoreceptors including the G protein coupled receptor (GPCR). Previous studies suggested that, through the GPCR signaling pathway, the secretion of GLP-1 can be induced in NCI-H716. Accordingly, we studied the GLP-1 stimulation effect of the AA extract and its mode-of-action using the GLP-1 ELISA and microarray. Functional categorization of the microarray data confirmed up or down-regulated gene expressions associated with the GPCR signaling pathway. This study demonstrates that AA extracts have a scientific possibility as a GLP-1 stimulant and thus may have the potential to be a therapeutic herbal medicine for type II diabetes mellitus.

Keywords

Anemarrhena asphodeloides (AA) Glucagonlike peptide-1 (GLP-1) Enteroendocrine cell NCI-H716 Type II diabetes mellitus Herbal medicine 
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References

  1. 1.
    Hui, H., Tang, G. & Go, V.L. Hypoglycemic herbs and their action mechanisms. Chin. Med. 4, 11 (2009).CrossRefGoogle Scholar
  2. 2.
    Bolen, S. et al. Systematic review: comparative effectiveness and safety of oral medications for type 2 diabetes mellitus. Ann. Intern. Med. 147, 386–399 (2007).CrossRefGoogle Scholar
  3. 3.
    Jang, H.J. et al. Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1. Proc. Natl. Acad. Sci. USA 104, 15069–15074 (2007).CrossRefGoogle Scholar
  4. 4.
    Le Neve, B. & Daniel, H. Selected tetrapeptides lead to a GLP-1 release from the human enteroendocrine cell line NCI-H716. Regul. Pept. 167, 14–20 (2011).CrossRefGoogle Scholar
  5. 5.
    Kreymann, B., Williams, G., Ghatei, M.A. & Bloom, S.R. Glucagon-like peptide-1 7–36: a physiological incretin in man. Lancet 2, 1300–1304 (1987).CrossRefGoogle Scholar
  6. 6.
    Wettergren, A. et al. Truncated GLP-1 (proglucagon 78–107-amide) inhibits gastric and pancreatic functions in man. Dig. Dis. Sci. 38, 665–673 (1993).CrossRefGoogle Scholar
  7. 7.
    D’Alessio, D.A., Kahn, S.E., Leusner, C.R. & Ensinck, J.W. Glucagon-like peptide 1 enhances glucose tolerance both by stimulation of insulin release and by increasing insulin-independent glucose disposal. J. Clin. Invest. 93, 2263–2266 (1994).CrossRefGoogle Scholar
  8. 8.
    Todd, J.F. et al. Glucagon-like peptide-1 (GLP-1): a trial of treatment in non-insulin-dependent diabetes mellitus. Eur. J. Clin. Invest. 27, 533–536 (1997).CrossRefGoogle Scholar
  9. 9.
    Venkatakrishnan, A.J. et al. Molecular signatures of G-protein-coupled receptors. Nature 494, 185–194 (2013).CrossRefGoogle Scholar
  10. 10.
    Kinnamon, S.C. Taste receptor signalling-from tongues to lungs. Acta Physiol. (Oxf ) 204, 158–168 (2012).CrossRefGoogle Scholar
  11. 11.
    Kim, S.J. et al. Pancreatic beta-cell prosurvival effects of the incretin hormones involve post-translational modification of Kv2.1 delayed rectifier channels. Cell Death Differ. 19, 333–344 (2012).CrossRefGoogle Scholar
  12. 12.
    Hoa, N.K., Phan, D.V., Thuan, N.D. & Ostenson, C.G. Insulin secretion is stimulated by ethanol extract of Anemarrhena asphodeloides in isolated islet of healthy Wistar and diabetic Goto-Kakizaki Rats. Exp. Clin. Endocrinol. Diabetes. 112, 520–525 (2004).CrossRefGoogle Scholar
  13. 13.
    Choi, E.K. et al. Hexane fraction of Citrus aurantium L. stimulates glucagon-like peptide-1 (GLP-1) secretion via membrane depolarization in NCI-H716 cells. BioChip J. 6, 41–47 (2012).CrossRefGoogle Scholar
  14. 14.
    Shin, M.H. et al. Gentiana scabra extracts stimulate glucagon-like peptide-1 secretion via G protein-coupled receptor pathway. BioChip J. 6, 114–119 (2012).CrossRefGoogle Scholar
  15. 15.
    Shi, C.S. et al. Regulator of G-protein signaling 3 (RGS3) inhibits Gbeta1gamma 2-induced inositol phosphate production, mitogen-activated protein kinase activation, and Akt activation. J. Biol. Chem. 276, 24293–24300 (2001).CrossRefGoogle Scholar
  16. 16.
    von Buchholtz, L. et al. RGS21 is a novel regulator of G protein signalling selectively expressed in subpopulations of taste bud cells. Eur. J. Neurosci. 19, 1535–1544 (2004).CrossRefGoogle Scholar
  17. 17.
    Bennett, V. & Baines, A.J. Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues. Physiol. Rev. 81, 1353–1392 (2001).Google Scholar
  18. 18.
    Stabach, P.R., Devarajan, P., Stankewich, M.C., Bannykh, S. & Morrow, J.S. Ankyrin facilitates intracellular trafficking of alpha1-Na+-K+-ATPase in polarized cells. Am. J. Physiol. Cell. Physiol. 295, C1202–1214 (2008).CrossRefGoogle Scholar
  19. 19.
    Singleton, P.A. & Bourguignon, L.Y. CD44 interaction with ankyrin and IP3 receptor in lipid rafts promotes hyaluronan-mediated Ca2+ signaling leading to nitric oxide production and endothelial cell adhesion and proliferation. Exp. Cell Res. 295, 102–118 (2004).CrossRefGoogle Scholar
  20. 20.
    Tolhurst, G. et al. Glutamine triggers and potentiates glucagon-like peptide-1 secretion by raising cytosolic Ca2+ and cAMP. Endocrinology 152, 405–413 (2011).CrossRefGoogle Scholar
  21. 21.
    Chung, H.J., Jan, Y.N. & Jan, L.Y. Polarized axonal surface expression of neuronal KCNQ channels is mediated by multiple signals in the KCNQ2 and KCNQ3 C-terminal domains. Proc. Natl. Acad. Sci. USA 103, 8870–8875 (2006).CrossRefGoogle Scholar
  22. 22.
    Choi, E.-K. et al. Genome-wide gene expression analysis of Patrinia scabiosaefolia reveals an antibiotic effect. BioChip J. 5, 246–254 (2011).CrossRefGoogle Scholar
  23. 23.
    Kim, K.-S. et al. Global transcriptome analysis of the Escherichia coli O157 response to Houttuynia Cordata Thunb. BioChip J. 4, 237–246 (2010).CrossRefGoogle Scholar
  24. 24.
    Kim, K.-S. et al. The multi-target antibiotic efficacy of Angelica dahurica Bentham et Hooker extract exposed to the Escherichia coli O157:H7. BioChip J. 5, 333–342 (2011).CrossRefGoogle Scholar
  25. 25.
    Yang, H.J. et al. Global transcriptome analysis of the E. coli O157 response to Agrimonia pilosa extract. Mol. Cell. Toxicol. 7, 299–310 (2011).CrossRefGoogle Scholar

Copyright information

© The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Kang-Hoon Kim
    • 1
  • Ki-Suk Kim
    • 1
  • Min Hee Shin
    • 1
  • Eun Gyeong Jang
    • 1
  • Eun Young Kim
    • 1
  • Jang-Hoon Lee
    • 1
  • Kwang Seok Ahn
    • 1
  • Jae-Young Um
    • 1
  • Hyeung-Jin Jang
    • 1
  1. 1.College of Korean Medicine, Institute of Korean MedicineKyung Hee UniversitySeoulKorea

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