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The mechanism of improved gellan gum production by two-stage culture of Sphingomonas paucimobilis

Abstract

A two-stage culture (with controlled sucrose concentrations and temperatures) of Sphingomonas paucimobilis for gellan gum production has been previously investigated. Herein, the mechanism of a two-stage culture favoring gellan gum overproduction was revealed by analysing the cell-membrane permeability and the proteomics for gellan gum biosynthesis. The two-stage culture, resulted in 79.8% increased content of unsaturated fatty acids, and 3.95% increased ratio of unsaturated to saturated fatty acids in the cell membrane. Moreover, cell membrane permeability increased and thus further enhanced gellan gum biosynthesis. Proteomic analysis results indicated that 13 identified protein spots were involved in energy generation, glycogen biosynthesis, and glycolysis. These findings revealed that two-stage culture impellel carbon flux flow toward gellan gum biosynthesis.

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Acknowledgements

We are grateful for financial support from the National Natural Science Foundation of China (31401657).

Author information

GZ is responsible for experiment design. XC is responsible for revise the manuscript. ZZ is responsible for data analysis. ZF is responsible for instrument operation. QT is responsible for providing overall ideas.

Correspondence to Guilan Zhu.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Zhu, G., Cheng, X., Fu, Z. et al. The mechanism of improved gellan gum production by two-stage culture of Sphingomonas paucimobilis. 3 Biotech 10, 70 (2020). https://doi.org/10.1007/s13205-019-2047-3

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Keywords

  • Two-stage culture
  • Gellan gum
  • Sphingomonas paucimobilis
  • Proteomics