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Bacillus  firmus  strain  FSS2C  ameliorated  oxidative  stress  in  wheat  plants  induced  by  azo  dye  (reactive  black-5)

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Abstract

This study was conducted to determine the ability of a bacterial strain FSS2C to ameliorate growth of wheat plants grown under induced stress of reactive black-5 (RB-5). The strain was taxonomically identified as Bacillus firmus on the basis of its 16S rRNA gene sequence analysis. The B. firmus FSS2C was found physiologically potent in phosphate solubilization, indole-3-acetic acid production and ammonia synthesis in the presence of varying concentrations of azo dye RB-5. Moreover, it decolorized RB-5 in vitro with the maximum decolorization (%) found at pH 7 and 30 °C. Inoculation of wheat plants, growing under stress induced by RB-5 dye, with rifampicin-resistant derivatives of the strain FSS2C substantially reduced the cellular oxidative stress, thereby resulting in higher plant biomass as compared to non-inoculated plants. Similarly, the inoculated plants revealed higher nutrient content in shoots as compared to non-inoculated ones. It was concluded that B. firmus strain FSS2C alleviated the oxidative stress impairment caused by reactive black-5 in wheat plants. Therefore, the strain can be used as bio-inoculant in wastewater irrigated soils.

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Acknowledgements

This research work was partially supported by the “HEC Start-up Research Grant # 21-1093/SRGP/R&D/HEC/2016”. The Department of Bioinformatics and Biotechnology is also highly acknowledged for the financial support from Departmental annual Grant.

Funding

This present research work was partially supported by the “HEC Start-up Research Grant # 21-1093/SRGP/R&D/HEC/2016”. The Department of Bioinformatics and Biotechnology is also highly acknowledged for the financial support from Departmental annual Grant.

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Correspondence to Muhammad Shahid.

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Mahmood, F., Shahid, M., Hussain, S. et al. Bacillus  firmus  strain  FSS2C  ameliorated  oxidative  stress  in  wheat  plants  induced  by  azo  dye  (reactive  black-5). 3 Biotech 10, 40 (2020). https://doi.org/10.1007/s13205-019-2031-y

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Keywords

  • Abiotic stress
  • Antioxidants
  • Azo dyes
  • B. firmus
  • Oxidative damage
  • Wheat