3 Biotech

, 10:41 | Cite as

Vitamin D2 fortification of Calocybe indica mushroom by natural and artificial UVB radiations and their potential effects on nutraceutical properties

  • Himanshi Rathore
  • Shalinee Prasad
  • Sneha Sehwag
  • Satyawati SharmaEmail author
Original Article


Calocybe indica mushroom was exposed under natural and artificial UVB light to enhance vitamin D2 contents in the fruit bodies. When Kinetic model was designed to examine the rate of conversion of ergosterol into vitamin D2 at different time intervals (0, 15, 45, 60 and 90 min), it was found that the conversion was linear with time. The maximum content of vitamin D2, that is 78.33 µg/g in sunlight and 140.58 µg/g in UVB radiated fruit bodies, was recorded in the samples exposed for 60 min. Interestingly, UVB radiations triggered the synthesis of β-glucan from their actual content (22.42–44.36 g/100 g) and improved the contents of phenols (12.46–47.38 mg GAE/g) and flavonoids (0.85–2.15 mg Quercetin/g). The estimated antioxidant activities, viz., free radical DPPH scavenging activity and ferric reducing antioxidant power was also found to significantly (p < 0.05) increase after 60 min of UVB exposure. For DPPH and FRAP, lowest IC50 values obtained was 1.90 and 4.60 respectively, which are suggestive of high antioxidant capacity. Additionally, the paper also describes how UVB rays chemically altered the scores for all seventeen amino acids that were analyzed.


Amino acids Antioxidants β-Glucan Nutraceutical Vitamin D2 



The authors grateful acknowledge the financial support provided by the University Grant Commission (UGC) (Grant no. 2201011-NETJRF-10299-69) and National Horticulture Board (NHB) for carrying out the research work.

Compliance with ethical standards

Conflict of interest

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Supplementary material

13205_2019_2024_MOESM1_ESM.docx (72 kb)
Supplementary file1 (DOCX 71 kb)


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Copyright information

© King Abdulaziz City for Science and Technology 2020

Authors and Affiliations

  1. 1.Centre for Rural Development and TechnologyIndian Institute of Technology DelhiHauz KhasIndia

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