3 Biotech

, 9:440 | Cite as

Impact of immobilization technology in industrial and pharmaceutical applications

  • Mohamed E. HassanEmail author
  • Qingyu Yang
  • Zhigang XiaoEmail author
  • Lu Liu
  • Na Wang
  • Xiaotong Cui
  • Liu Yang
Review Article


The current demands of the world’s biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. Compared to free enzymes in solution, immobilized enzymes are more robust and more resistant to environmental changes. More importantly, the heterogeneity of the immobilized enzyme systems allows an easy recovery of both enzymes and products, multiple reuse of enzymes, continuous operation of enzymatic processes, rapid termination of reactions, and greater variety of bioreactor designs. This review summarizes immobilization definition, different immobilization methods, advantages and disadvantages of each method. In addition, it covers some food industries, protein purification, human nutrition, biodiesel production, and textile industry. In these industries, the use of enzymes has become an inevitable processing strategy when a perfect end product is desired. It also can be used in many other important industries including health care and pharmaceuticals applications. One of the best uses of enzymes in the modern life is their application in diagnose and treatment of many disease especially when used in drug delivery system or when used in nanoform.


Immobilization methods Industrial applications Pharmaceutical applications Nanoimmobilization 



The authors gratefully acknowledge the financial support of Shenyang Major S&T Achievements Transformation Program (Z18-5-019) and Distinguished Professor Program of Liaoning province (Key technology research and new product creation of potato main food manufacturing).

Compliance with ethical standards

Conflict of interest

There is no conflict of interest.


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© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.College of Grain Science and TechnologyShenyang Normal UniversityShenyangChina
  2. 2.Center of Excellence, Encapsulation and Nano Biotechnology Group, Chemistry of Natural and Microbial Products DepartmentNational Research CenterCairoEgypt

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