An “olivomycin A” derivative from a sponge-associated Streptomyces sp. strain SP 85
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We isolated an actinobacterium, Streptomyces sp. strain SP 85 from the marine sponge Dysidea avara. Polyphasic identification of the microorganism showed that the strain SP 85 had high 16S rRNA gene similarity (99%) with Streptomyces olivaceus strain NBRC 12805, while some physiological and biochemical differences were observed. A cytotoxic compound, SP 85 was isolated from the active culture extract of the strain SP 85 by bioassay-guided purification over silica gel column chromatography, preparative TLC, and HPLC. The structure elucidation based on the spectroscopic analysis, including UV, ESI–MS, and 13C NMR data revealed that SP 85 compound is an analog of anti-tumor drug, “olivomycin A”. The SP 85 compound showed high cytotoxic activity against three human cancer cell lines, including SW480, HepG2, and MCF7 with IC50 values of 16, 93, and 78 nM, respectively. SP 85 exhibited significantly (2–10 times) higher cytotoxicity against the tumor cell lines in comparison with HUVECs as the normal cell line, which also induced apoptosis in the tested cancerous cell line. This is the first report on the production of an “olivomycin A” derivative by a sponge-associated Streptomyces, showing the great potential of sponge-associated actinobacteria in producing cytotoxic natural products.
KeywordsSponge-associated Streptomyces Dysidea avara Olivomycin Anti-tumor antibiotics Phylogenetic analysis
The authors are thankful to Prof. Bernd Schneider at Max Planck Institute for chemical ecology, Jena, Germany for the NMR measurement, Mrs. Najmeh Khalighian for her technical help and research council of Shiraz University of medical sciences for using the analytical measurements. The authors wish to thank Mr. H. Argasi at the Research Consultation Center (RCC) of Shiraz University of Medical Sciences for his invaluable assistance in editing this manuscript.
MG designed and preformed the isolation, screening, and identification of bacterial strain under the supervision of NB. Bioassays were carried out under the supervision of EE. MG performed extraction and purification of the bioactive compound under the guidance of ARJ. ARJ designed and analyzed the spectroscopic analysis and elucidation of the bioactive compound. The manuscript was written by MG and revised by ARJ, NB, MSM, and EE.
This research article is a part of the PhD thesis entitled “Evaluation, identification and structure elucidation of cytotoxic and antioxidant secondary metabolites from Persian Gulf and Oman Sea derived Actinobacteria” that was supported by Islamic Azad University, Shiraz branch.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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