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3 Biotech

, 9:423 | Cite as

Repetitive genomic elements in Campomanesia xanthocarpa: prospection, characterization and cross amplification of molecular markers

  • Vanessa S. Petry
  • Valdir Marcos Stefenon
  • Lilian O. Machado
  • Gustavo H. F. Klabunde
  • Fábio O. Pedrosa
  • Rubens O. NodariEmail author
Original Article
First Online:
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Abstract

Repetitive genomic elements were prospected in Campomanesia xanthocarpa, aiming to characterize these elements in a non-model plant species and to develop species-specific microsatellite markers. Approximately 4.12% of the partial genome of C. xanthocarpa is composed of repetitive elements, being retrotransposons the most widely represented. A total of nine polymorphic microsatellite markers were obtained: four nuclear-neutral, two nuclear EST, two plastidial and one mitochondrial. Levels of population genetic diversity of four natural populations of C. xanthocarpa were characterized using these markers. In addition, the cross-species amplification of the microsatellite markers was tested in seven species of tribe Myrteae (Myrtaceae). The characterized microsatellite markers revealed low to moderate levels of genetic diversity (expected heterozygosity range: 0.33–0.57; observed heterozygosity: 0.26–0.74 and number of alleles: 2.25–4.25). Cross-species amplification was successful for all loci, except Cxant76. These nine markers will contribute for studies on genetic diversity, gene flow, plant selection and breeding of this species, towards the conservation of natural populations, as well as its commercial use.

Keywords

Guabiroba nSSRs ptSSRs mtSSRs Transferability Transposons 
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Notes

Acknowledgements

The authors would like to thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support (Proc. 307144/2013-5) and scholarships and grants awarded to V.M.S. (Process 113617/2018-6), V.S.P. and R.O.N. The authors would also like to thank CAPES for scholarships awarded to G.H.F.K. and L.O.M., and to the Nucleus of Nitrogen Fixation/UFPR for sequencing. We thank Ms. Yohan Fritsche (LFDGV, UFSC) for the help with the flow cytometry analysis.

Compliance with ethical standards

Ethical approval

This article does not include any studies with human participants or animals performed by any of the authors.

Informed consent

This article does not involve any informed consent.

Conflict of interest

All authors hereby declare that there is no conflict of interest.

Supplementary material

13205_2019_1953_MOESM1_ESM.pdf (554 kb)
Supplementary material 1 (PDF 553 kb)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Graduate Program in Plant Genetic ResourcesUniversidade Federal de Santa CatarinaFlorianópolisBrazil
  2. 2.Nucleus of Molecular Ecology and Plant MicropropagationUniversidade Federal do PampaSão GabrielBrazil
  3. 3.Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (EPAGRI)ItajaíBrazil
  4. 4.Department of Biochemistry and Molecular Biology, Nucleus of Nitrogen FixationUniversidade Federal do ParanáCuritibaBrazil

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