3 Biotech

, 9:399 | Cite as

Anticancer activity of plant leaves extract collected from a tribal region of India

  • Gourav Kumar
  • Rashmi Gupta
  • Shruti Sharan
  • Partha Roy
  • Dev Mani PandeyEmail author
Original Article


The goal of this research was to explore the preliminary anticancer properties of five plants namely Calotropis procera, Moringa oleifera, Millettia pinnata, Basela alba and Euphorbia neriifolia available in Jharkhand which is used for the medicinal purpose by local tribes. In the present study, plant leaves from five species were collected, dried and extracted with solvents of increasing polarity, followed by assessment of their cytotoxicity in A549 non-small-cell lung cancer cells. In the antimicrobial assay, the methanol extract of the M. pinnata leaves exhibited comparatively higher zone of inhibition of 0.7 ± 0.20 cm against a Salmonella typhi culture than the other extracts. M. pinnata leaves extract also displayed the maximum percentage inhibition in the DPPH, 83.97 ± 0.01 FRAP, 193.14 ± 3.01 mM assays. Furthermore, the cytotoxicity of the chloroform (37.45 ± 1.04) and ethyl acetate extracts (34.20 ± 0.81) of M. pinnata against A549 cells was found relatively higher with respect to another extract. In contrast, a study with the L132 normal epithelial lung cell line revealed less toxicity from the chloroform extract (0.33 ± 0.19) compared to the ethyl acetate extract (6.65 ± 0.59). Based on these findings, phytochemical investigation on chloroform and ethyl acetate extract of M. pinnata was performed using UPLC-ESI–MS/MS analysis revealing the presence of β-sitosterol, lanceolatin B, karanjin, and stigmasterol. Congruently, a complete phytochemical and cytotoxic investigation of the M. pinnata extract constituents might infer the potency of this extract/s as anticancer, antioxidant and antimicrobial agents.


Anticancer Antioxidant assay Apoptosis Mass spectroscopy 





Acridine orange/ethidium bromide


4′,6-Diamidino-2-phenylindole, dihydro chloride


Ferric reducing ability of plasma




Mass-to-charge ratio












2,2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid)




Dulbecco’s modified eagle’s medium


Foetal bovine serum


Enzyme-linked immunosorbent assay


Optical density


Zone of inhibition




Standard deviation


50% Inhibitory concentration




Nicotinamide adenine dinucleotide phosphate



Centre of Excellence (COE) TEQIP-II Grant no- NPIU/TEQIP II/FLN/31/158 is gratefully acknowledged for providing financial assistance to GK in the form of scholarship. Financial assistance provided by BIT Mesra, Ranchi, India to DMP through Seed Money Scheme-2015 (Ref.GO/SMS/DSR-007/2015-2016 dated 13-11-2015) is gratefully acknowledged. Central Instrumentation Facility BIT, Mesra, Ranchi, Jharkhand, India is acknowledged for providing analysis facility of the present study. Prof Shishir Sinha, Department of Chemical Engineering, IIT Roorkee is gratefully acknowledged for his support and suggestions. Authors wish to express a special gratitude to Dr. Stefano Dall’Acqua, Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Italy for proofreading of manuscript and valuable suggestions in removing the technical and grammatical errors.

Author contributions

All authors have seen and approved the manuscript and its contents, and that they are aware of the responsibilities connected to authorship. All the work was majorly performed by Mr. Gourav Kumar, under the supervision of Dr. D. M. Pandey, Associate Professor Department of Bio-Engineering, BIT Mesra, and with joint efforts of Ms. Rashmi Gupta, Department of Bio-Engineering, BIT Mesra. All cytotoxicity assays were performed at Indian Institute of Technology Roorkee, India under the guidance of Dr. Shruti Sharan and Dr. Partha Roy, Professor Department of Biotechnology.

Compliance with ethical standards

Conflict of interest

We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.

Ethical statement

We confirm that this research study does not include either vertebrates or higher invertebrates.

Supplementary material

13205_2019_1927_MOESM1_ESM.doc (7.3 mb)
Supplementary material 1 (DOC 7509 kb)


  1. Al Muqarrabun LMR, Ahmat N, Ruzaina SAS et al (2013) Medicinal uses, phytochemistry and pharmacology of Pongamia pinnata (L.) Pierre: a review. J Ethnopharmacol 150(2):395–420CrossRefGoogle Scholar
  2. Ansil PN, Wills PJ, Varun R et al (2014) Cytotoxic and apoptotic activities of Amorphophallus campanulatus (Roxb.) Bl. tuber extracts against human colon carcinoma cell line HCT-15. Saudi J Biol Sci 21(6):524–531CrossRefGoogle Scholar
  3. Atabani AE, Silitonga AS, Badruddin IA et al (2012) A comprehensive review on biodiesel as an alternative energy resource and its characteristics. Renew Sustain Energy Rev 16(4):2070–2093CrossRefGoogle Scholar
  4. Bernardo MA, Silva ML, Santos E et al (2015) Effect of cinnamon tea on postprandial glucose concentration. J Diabetes Res 2015:913651. CrossRefPubMedPubMedCentralGoogle Scholar
  5. Bhaskar VH, Ajay SS (2009) Antimicrobial activity of Calotropis procera seeds. Asian J Chem 21(7):5788Google Scholar
  6. Brand-Williams W, Cuvelier ME, Berset CLWT (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 28(1):25–30CrossRefGoogle Scholar
  7. Bukar A, Uba A, Oyeyi T (2010) Antimicrobial profile of Moringa oleifera Lam. extracts against some food–borne microorganisms. Bayero J Pure Appl Sci 3(1):43–48Google Scholar
  8. Chang LC, Gerhäuser C, Song L et al (1997) Activity-guided isolation of constituents of Tephrosia purpurea with the potential to induce the phase II enzyme, quinine reductase. J Nat Prod 60(9):869–873CrossRefGoogle Scholar
  9. Ding Y, Nguyen HT, Kim SI et al (2009) The regulation of inflammatory cytokine secretion in macrophage cell line by the chemical constituents of Rhus sylvestris. Bioorg Med Chem Lett 19(13):3607–3610CrossRefGoogle Scholar
  10. Gahlaut A, Chhillar AK (2013) Evaluation of antibacterial potential of plant extracts using resazurin based microtiter dilution assay. Int J Pharm Pharm Sci 5(2):372–376Google Scholar
  11. Galib MB, Mashru M, Jagtap C et al (2011) Therapeutic potentials of metals in ancient India: a review through CharakaSamhita. J Ayurveda Integr Med 2(2):55CrossRefGoogle Scholar
  12. Guo JR, Chen QQ, Lam CWK et al (2015) Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells. Biol Res 48(1):40CrossRefGoogle Scholar
  13. Hawk MA, McCallister C, Schafer ZT (2016) Antioxidant activity during tumor progression: a necessity for the survival of cancer cells? Cancers 8(10):92. CrossRefPubMedCentralGoogle Scholar
  14. Kondhare D, Lade H (2017) Phytochemical profile, aldose reductase inhibitory, and antioxidant activities of indian traditional medicinal Coccinia grandis (L.) fruit extract. 3 Biotech 7:378CrossRefGoogle Scholar
  15. Kumar R, Sharma M (2018) Herbal nanomedicine interactions to enhance pharmacokinetics, pharmaco-dynamics, and therapeutic index for better bioavailability and biocompatibility of herbal formulations. J Mater NanoSci 5(1):35–58Google Scholar
  16. Kumar G, Ghosh M, Pandey DM (2019) Method development for optimized green synthesis of gold nanoparticles from Millettia pinnata and their activity in Non-small cell lung cancer cell lines. IET Nanobiotechnol 13(6):626–633CrossRefGoogle Scholar
  17. Li S, Chen G, Zhang C et al (2014) Research progress of natural antioxidants in foods for the treatment of diseases. Food Science Hum Wellness 3(3):110–116CrossRefGoogle Scholar
  18. Mako GA, Memon AH, Mughal UR et al (2012) Antibacterial effects of leaves and root extract of Calotropis procera Linn. Pak J Agri Engg Vet Sci 28:141–149Google Scholar
  19. Nikhil K, Sharan S, Chakraborty A et al (2014) Role of isothiocyanate conjugate of pterostilbene on the inhibition of MCF-7 cell proliferation and tumor growth in Ehrlich ascitic cell induced tumor bearing mice. Exp Cell Res 320(2):311–328CrossRefGoogle Scholar
  20. Panat NA, Maurya DK, Ghaskadbi SS et al (2016) Troxerutin, a plant flavonoid, protects cells against oxidative stress-induced cell death through radical scavenging mechanism. Food Chem 194:32–45CrossRefGoogle Scholar
  21. Perez C, Pauli M, Bazerque P (1990) An antibiotic assay by the agar well diffusion method. Acta Biol Med Exp 15(1):113–115. CrossRefGoogle Scholar
  22. Pracheta SV, Paliwal R, Sharma S (2011) In vitro free radical scavenging and antioxidant potential of ethanolic extract of Euphorbia neriifolia Linn. Int J Pharm PharmSci 3(1):238–242Google Scholar
  23. Rahman MM, Sheikh MMI, Sharmin SA et al (2009) Antibacterial activity of leaf juice and extracts of Moringa oleifera Lam against some human pathogenic bacteria. CMU J Nat Sci 8(2):219Google Scholar
  24. Re R, Pellegrini N, Proteggente A et al (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26(9):1231–1237CrossRefGoogle Scholar
  25. Roy N, Laskar RA, Sk I et al (2011) A detailed study on the antioxidant activity of the stem bark of Dalbergia sissoo Roxb., an Indian medicinal plant. Food Chem 126(3):1115–1121CrossRefGoogle Scholar
  26. Sajid ZI, Anwar F, Shabir G et al (2012) Antioxidant, antimicrobial properties and phenolics of different solvent extracts from bark, leaves and seeds of Pongamia pinnata (L.) Pierre. Molecules 17(4):3917–3932CrossRefGoogle Scholar
  27. Sayin VI, Ibrahim MX, Larsson E et al (2014) Antioxidants accelerate lung cancer progression in mice. Sci Transl Med 6(221):221ra15–221ra15CrossRefGoogle Scholar
  28. Shamim G, Ranjan SK, Pandey DM et al (2016) Lac dye as a potential anti-neoplastic agent. J Cancer Res Ther 12(2):1033. CrossRefPubMedGoogle Scholar
  29. Sharma S, Sundararajan M, Kumar R (2016) Medicinal plant in Jharkhand State: an overview of current scenario. Global J Eng Sci Res 1–6. ISSN 2348-8034.
  30. Shen T, Zhang L, Wang YY et al (2012) Steroids from Commiphora mukul display antiproliferative effect against human prostate cancer PC3 cells via induction of apoptosis. Bioorg Med Chem Lett 22(14):4801–4806CrossRefGoogle Scholar
  31. Surveswaran S, Cai YZ, Xing J et al (2010) Antioxidant properties and principal phenolic phytochemicals of Indian medicinal plants from Asclepiadoideae and Periplocoideae. Nat Prod Res 24(3):206–221CrossRefGoogle Scholar
  32. Sushila R, Deepti A, Permender R et al (2010) Cytotoxic and antibacterial activity of Basella alba whole plant: a relatively unexplored plant. Pharmacologyonline 3:651–658Google Scholar
  33. Tiwari R, Latheef SK, Ahmed I et al (2018) Herbal immunomodulators- a remedial panacea for designing and developing effective drugs and medicines: current scenario and future prospects. Curr Drug Metab 19(3):264–301CrossRefGoogle Scholar
  34. Zhao ZQ, Yu ZY, Li J et al (2016) Gefitinib induces lung cancer cell autophagy and apoptosis via blockade of the PI3 K/AKT/mTOR pathway. Oncol Lett 12(1):63–68CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Bio-EngineeringBirla Institute of TechnologyMesraIndia
  2. 2.Department of Bio-TechnologyIndian Institute of TechnologyRoorkeeIndia

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