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3 Biotech

, 9:399 | Cite as

Anticancer activity of plant leaves extract collected from a tribal region of India

  • Gourav Kumar
  • Rashmi Gupta
  • Shruti Sharan
  • Partha Roy
  • Dev Mani PandeyEmail author
Original Article
  • 36 Downloads

Abstract

The goal of this research was to explore the preliminary anticancer properties of five plants namely Calotropis procera, Moringa oleifera, Millettia pinnata, Basela alba and Euphorbia neriifolia available in Jharkhand which is used for the medicinal purpose by local tribes. In the present study, plant leaves from five species were collected, dried and extracted with solvents of increasing polarity, followed by assessment of their cytotoxicity in A549 non-small-cell lung cancer cells. In the antimicrobial assay, the methanol extract of the M. pinnata leaves exhibited comparatively higher zone of inhibition of 0.7 ± 0.20 cm against a Salmonella typhi culture than the other extracts. M. pinnata leaves extract also displayed the maximum percentage inhibition in the DPPH, 83.97 ± 0.01 FRAP, 193.14 ± 3.01 mM assays. Furthermore, the cytotoxicity of the chloroform (37.45 ± 1.04) and ethyl acetate extracts (34.20 ± 0.81) of M. pinnata against A549 cells was found relatively higher with respect to another extract. In contrast, a study with the L132 normal epithelial lung cell line revealed less toxicity from the chloroform extract (0.33 ± 0.19) compared to the ethyl acetate extract (6.65 ± 0.59). Based on these findings, phytochemical investigation on chloroform and ethyl acetate extract of M. pinnata was performed using UPLC-ESI–MS/MS analysis revealing the presence of β-sitosterol, lanceolatin B, karanjin, and stigmasterol. Congruently, a complete phytochemical and cytotoxic investigation of the M. pinnata extract constituents might infer the potency of this extract/s as anticancer, antioxidant and antimicrobial agents.

Keywords

Anticancer Antioxidant assay Apoptosis Mass spectroscopy 

Abbreviations

DPPH

1,1-Diphenyl-2-picrilhydrazyl

AO/EtBr

Acridine orange/ethidium bromide

DAPI

4′,6-Diamidino-2-phenylindole, dihydro chloride

FRAP

Ferric reducing ability of plasma

h

Hour/hours

m/z

Mass-to-charge ratio

μL

Microlitre

mg

Milligram

mL

Millilitre

μg

Microgram

mM

Millimolar

ABTS+

2,2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid)

TPTZ

Tripyridyltriazine

DMEM

Dulbecco’s modified eagle’s medium

FBS

Foetal bovine serum

ELISA

Enzyme-linked immunosorbent assay

OD

Optical density

ZOI

Zone of inhibition

cm

Centimetre

STDEVA

Standard deviation

IC50

50% Inhibitory concentration

UV–Vis

Ultraviolet–visible

NADPH

Nicotinamide adenine dinucleotide phosphate

Notes

Acknowledgement

Centre of Excellence (COE) TEQIP-II Grant no- NPIU/TEQIP II/FLN/31/158 is gratefully acknowledged for providing financial assistance to GK in the form of scholarship. Financial assistance provided by BIT Mesra, Ranchi, India to DMP through Seed Money Scheme-2015 (Ref.GO/SMS/DSR-007/2015-2016 dated 13-11-2015) is gratefully acknowledged. Central Instrumentation Facility BIT, Mesra, Ranchi, Jharkhand, India is acknowledged for providing analysis facility of the present study. Prof Shishir Sinha, Department of Chemical Engineering, IIT Roorkee is gratefully acknowledged for his support and suggestions. Authors wish to express a special gratitude to Dr. Stefano Dall’Acqua, Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Italy for proofreading of manuscript and valuable suggestions in removing the technical and grammatical errors.

Author contributions

All authors have seen and approved the manuscript and its contents, and that they are aware of the responsibilities connected to authorship. All the work was majorly performed by Mr. Gourav Kumar, under the supervision of Dr. D. M. Pandey, Associate Professor Department of Bio-Engineering, BIT Mesra, and with joint efforts of Ms. Rashmi Gupta, Department of Bio-Engineering, BIT Mesra. All cytotoxicity assays were performed at Indian Institute of Technology Roorkee, India under the guidance of Dr. Shruti Sharan and Dr. Partha Roy, Professor Department of Biotechnology.

Compliance with ethical standards

Conflict of interest

We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.

Ethical statement

We confirm that this research study does not include either vertebrates or higher invertebrates.

Supplementary material

13205_2019_1927_MOESM1_ESM.doc (7.3 mb)
Supplementary material 1 (DOC 7509 kb)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Bio-EngineeringBirla Institute of TechnologyMesraIndia
  2. 2.Department of Bio-TechnologyIndian Institute of TechnologyRoorkeeIndia

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