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3 Biotech

, 9:398 | Cite as

Characterization and probiotic properties of Lactobacilli from human breast milk

  • Chanettee Jamyuang
  • Phanphen Phoonlapdacha
  • Nalinee Chongviriyaphan
  • Wasaporn Chanput
  • Sunee Nitisinprasert
  • Massalin NakphaichitEmail author
Original Article

Abstract

Several studies have reported a complex microbial community in human breast milk. This community impacts the shape of the infant gut microbiota and consequently impacts host health. Lactobacillus is an important probiotic and has many applications in the functional food industry. This study isolated and evaluated the potential probiotic bacteria from human milk. Two Lactobacillus species, L. plantarum and L. pentosus, were isolated from the breast milk of Thai women. L. pentosus HM04-22, L. pentosus HM04-3, L. plantarum HM04-80, L. plantarum HM04-88 and L. plantarum HM01-1 showed good adhesion activity (> 55%) and resistance in gastric (pH 2) and bile (pH 8) conditions. Characterization of the probiotic properties indicated that all selected Lactobacillus isolates had anti-adhesion properties against Escherichia coli and Salmonella Typhimurium. Lactobacillus isolates protected Caco-2 cells from pathogen adhesion at 25–40%. In addition, the five selected strains presented anti-inflammatory properties by reducing interleukin (IL)-8 expression at 0.14 ± 0.16 to 0.52 ± 0.117-fold. However, the strains had no effect on the expression of tight junction genes, including zona occludens (ZO)-1, occludin and claudin-1. In conclusion, five selected Lactobacillus isolates from human milk were candidates for use as probiotics to promote health. However, more tests in animal models and clinical trials need to be performed.

Keywords

Probiotic Human breast milk Adhesion Immune 

Notes

Acknowledgements

The authors would like to thank Assoc. Prof. Dr. Kwannan Nantavisai and Assist. Prof. Dr. Pakamon Chitprasert for the Caco-2 cell line. This study was funded by Kasetsart University Research and Development Institute fiscal year 2017.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Biotechnology, Faculty of Agro-IndustryKasetsart UniversityBangkokThailand
  2. 2.Department of Food Science, Faculty of Agro-IndustryKasetsart UniversityBangkokThailand
  3. 3.Division of Nutrition, Department of Pediatrics, Faculty of Medicine Ramathibodi HospitalMahidol UniversityBangkokThailand

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