3 Biotech

, 9:397 | Cite as

Physiological response of tomato plant to chitosan-immobilized aggregated Methylobacterium oryzae CBMB20 inoculation under salinity stress

  • Mak Chanratana
  • Manoharan Melvin Joe
  • Aritra Roy Choudhury
  • Rangasamy Anandham
  • Ramasamy Krishnamoorthy
  • Kiyoon Kim
  • Sunyoung Jeon
  • Joonho Choi
  • Jeongyun Choi
  • Tongmin SaEmail author
Original Article


The use of plant growth promoting bacteria as bioinoculant to alleviate salt stress is a sustainable and eco-friendly approach in agriculture. However, the maintenance of the bacterial population in the soil for longer period is a major concern. In the present study, chitosan-immobilized aggregated Methylobacterium oryzae CBMB20 was used as a bioinoculant to improve tomato plant (Solanum lycopersicum Mill.) growth under salt stress. The chitosan-immobilized aggregated M. oryzae CBMB20 was able to enhance plant dry weight, nutrient uptake (N, P, K and Mg2+), photosynthetic efficiency and decrease electrolyte leakage under salt stress conditions. The oxidative stress exerted by elevated levels of salt stress was also alleviated by the formulated bioinoculant, as it up-regulated the antioxidant enzyme activities and enhanced the accumulation of proline which acts as an osmolyte. The chitosan-immobilized aggregated M. oryzae CBMB20 was able to decrease the excess Na+ influx into the plant cells and subsequently decreasing the Na+/K+ ratio to improve tomato plant growth under salt stress conditions. Therefore, it is proposed that the chitosan-immobilized aggregated M. oryzae CBMB20 could be used as a bioinoculant to promote the plant growth under salt stress conditions.


Carrier material Enzyme activity Immobilization Methylobacterium oryzae CBMB20 Salt stress Tomato 



This work was supported by the Basic Science Research Program, National Research Foundation of Korea (NRF), Ministry of Education, Science and Technology [2015R1A2A1A05001885], Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that we have no competing interests.


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Mak Chanratana
    • 1
    • 6
  • Manoharan Melvin Joe
    • 1
    • 2
  • Aritra Roy Choudhury
    • 1
  • Rangasamy Anandham
    • 3
  • Ramasamy Krishnamoorthy
    • 4
  • Kiyoon Kim
    • 1
    • 7
  • Sunyoung Jeon
    • 1
  • Joonho Choi
    • 5
  • Jeongyun Choi
    • 1
  • Tongmin Sa
    • 1
    Email author
  1. 1.Department of Environmental and Biological ChemistryChungbuk National UniversityCheongjuRepublic of Korea
  2. 2.Department of Industrial MicrobiologySam Higginbottom University of Agriculture, Technology and SciencesAllahabadIndia
  3. 3.Department of Agricultural MicrobiologyTamil Nadu Agricultural UniversityCoimbatoreIndia
  4. 4.Department of Crop ManagementVanavarayar Institute of AgriculturePollachiIndia
  5. 5.Department of Food Science and BiotechnologyWonkwang UniversityIksanRepublic of Korea
  6. 6.Department of Plant Protection Sanitary and PhytosanitaryGeneral Directorate of Agriculture, Ministry of Agriculture, Forestry and FisheriesPhnom PenhCambodia
  7. 7.Forest Medicinal Resources Research CenterNational Institute of Forest ScienceYongjuRepublic of Korea

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