3 Biotech

, 9:378 | Cite as

Functional annotation and identification of MADS-box transcription factors related to tuber dormancy in Helianthus tuberosus L.

  • Shipeng Yang
  • Jieming Gao
  • Lihui Wang
  • Xuemei Sun
  • Panpan Xu
  • Liwen ZhangEmail author
  • Qiwen ZhongEmail author
Original Article


Dormancy-associated MADS-box (DAM) genes play an important role in plant dormancy and release phases. Little is known about the dormancy characteristics of Jerusalem artichoke tubers. Using bioinformatics, we identified and annotated 23 MADS-box gene sequences from the genome of the Jerusalem artichoke and we analyzed the differential expression of these genes at different developmental stages of tuber dormancy. The results show that all 23 genes encode basic proteins and most of the genes of the same subgroup have similar pI values. MADS-box genes from the Jerusalem artichoke and from other closely related species were divided into ten categories using phylogenetic analysis software. Based on the amino acid sequence of the MADS-domain proteins, ten highly conserved motifs were identified. Gene ontology annotation, InterProScan protein function prediction, and RT-PCR analysis showed that ten MADS-box genes play important roles in the dormancy process of Jerusalem artichoke tubers. Our work lays a foundation for further study of the role of MADS-box genes in the dormancy of the Jerusalem artichoke and other tuber crops.


Dormancy MADS-box transcription factors Gene expression Helianthus tuberosus L. 



Funding was provided by Qinghai Academy of Agriculture and Forestry Sciences Innovation Fund (Grant number: 2017-NKY-04), Natural Science Foundation of China (Grant number: 31660588; 31660569; 31760600), The Fundamental Research Program of Qinghai (Grant number: 2017-ZJ-Y18), The Project of Qinghai Science & Technology Department (Grant number: 2016-ZJ-Y01), The Open Project of State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University (Grant number: 2016-ZZ-06).

Compliance with ethical standards

Conflict of interest

On behalf of all the authors, the corresponding author states that there is no conflict of interest.


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Qinghai Key Laboratory of Vegetable Genetics and PhysiologyAcademy of Agriculture and Forestry Sciences of Qinghai University (Qinghai Academy of Agriculture and Forestry Sciences)XiningChina
  2. 2.Qinghai UniversityXiningChina
  3. 3.Shanghai Biochip Co., Ltd. & National Engineering Center for Biochip at ShanghaiShanghaiChina

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