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3 Biotech

, 9:332 | Cite as

Data-independent acquisition of the proteomics of spleens from chickens infected by avian leukosis virus

  • Fei Ye
  • Qijian He
  • Yan Wang
  • Can Cui
  • Fuling Yang
  • Bin Luo
  • Huadong Yin
  • Xiaoling Zhao
  • Diyan Li
  • Hengyong Xu
  • Hua LiEmail author
  • Qing ZhuEmail author
Original Article
  • 14 Downloads

Abstract

Immunosuppression caused by avian leukemia virus J subgroup (ALV-J) infection includes atrophy or regeneration disorders of the lymphoid organs, decreased immune response, and termination of B lymphocyte maturation process and inhibition of T-lymphocyte development. The regulatory mechanism of the related resistance genes and protein expression is not clear. While searching for a molecular marker for the immune response to ALV-J infection, we detected differentially expressed proteins (DEPs) of spleens from chicken infected by ALV-J at 15th day and 30th day by the data-independent acquisition technique. Approximately 220 DEPs from the spleens of chickens infected by ALV-J were detected. To find a relatively stable biomarker molecule, we summarized the DEPs at two timepoints and selected activating signal cointegrator 1 complex subunit 3 (ASCC3), TBC1 domain family member 2 (TBC1D2), MHC class II beta chain 1 (BLB2), ensconsin (MAP7), complement component 1 Q subcomponent B chain (C1QB), and Follistatin-like 1 (FSTL1) from both comparisons for protein interaction network analysis. ASCC3, BLB2, C1QB, and FSTL1 were potential biomarkers for the complex infection mechanism of ALV-J and the dynamic immune mechanism of the body.

Keywords

ALV-J Proteomic ASCC3 BLB2 C1QB FSTL1 

Notes

Acknowledgements

The Sequencing work was financially supported by the China Agriculture Research System (CARS-41), the Engineering Centre of Chicken Breeding in Guangdong Province (2017-1649) and Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding (2019B030301010). We would like to thank AMERICAN JOURNAL EXPERTS (https://secure.aje.com) for providing linguistic assistance during the preparation of this manuscript.

Author contributions

YW, HL, and QZ conceived and designed the experiments. FY and QJH performed the experiments. FY, CC, BL, FLY and HDY analyzed the data. HYX, DYL, and XLZ contributed the reagents/materials/analysis and tools. FY wrote the paper. YW, HL, and QZ revised the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Ethical statements

All animal care and experimental procedures were reviewed and approved by the Animal Care and Use Committee (#YYS130125) of the Animal Care Advisory at Sichuan Agricultural University. This study was carried out in strict accordance with the Regulations for the Administration of Affairs Concerning Experimental Animals of the State Council of the People’s Republic of China.

References

  1. Blum C, Graham A, Yousefzadeh M, Shrout J, Benjamin K, Krishna M, Hoda R, Hoda R, Cole DJ, Garrett-Mayer E (2008) The expression ratio of Map7/B2M is prognostic for survival in patients with stage II colon cancer. Int J Oncol 33(3):579–584PubMedPubMedCentralGoogle Scholar
  2. Brickner JR, Soll JM, Lombardi PM, Vågbø CB, Mudge MC, Oyeniran C, Rabe R, Jackson J, Sullender ME, Blazosky E (2017) A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair. Nature 551(7680):389PubMedPubMedCentralCrossRefGoogle Scholar
  3. Cesta MF (2006) Normal structure, function, and histology of the spleen. Toxicol Pathol 34(5):455–465PubMedCrossRefGoogle Scholar
  4. Clutter SD, Wilson DC, Marinov AD, Hirsch R (2009) Follistatin-like protein 1 promotes arthritis by up-regulating IFN-γ. J Immunol 182(1):234–239PubMedPubMedCentralCrossRefGoogle Scholar
  5. Cui Z, Sun S, Zhang Z, Meng S (2009) Simultaneous endemic infections with subgroup J avian leukosis virus and reticuloendotheliosis virus in commercial and local breeds of chickens. Avian Pathol 38(6):443–448PubMedCrossRefGoogle Scholar
  6. Desai MA, Jackson V, Zhai W, Suman SP, Nair MN, Beach CM, Schilling MW (2016) Proteome basis of pale, soft, and exudative-like (PSE-like) broiler breast (Pectoralis major) meat. Poult Sci 95(11):2696–2706PubMedCrossRefGoogle Scholar
  7. Fadly AM, Smith EJ (1999) Isolation and some characteristics of a subgroup J-like avian leukosis virus associated with myeloid leukosis in meat-type chickens in the United States. Avian Dis 43(3):391–400PubMedCrossRefGoogle Scholar
  8. Fan Z, Hu X, Zhang Y, Yu C, Qian K, Qin A (2012) Proteomics of DF-1 cells infected with avian leukosis virus subgroup. J Virus Res 167(2):314–321CrossRefGoogle Scholar
  9. Fu L, Fu H, Zhou L, Xu K, Pang Y, Hu K, Wang J, Tian L, Liu Y, Wang J (2016) High expression of MAP7 predicts adverse prognosis in young patients with cytogenetically normal acute myeloid leukemia. Sci Rep 6:34546PubMedPubMedCentralCrossRefGoogle Scholar
  10. Guo X-L, Zheng H-q, Li X-L, Li Y, Gu Z-L, Zheng C-S, Wei Z-H, Wang J-S, Zhou R-Y, Li L-H (2012) Genetic variation of major histocompatibility complex BLB2 gene exon 2 in Hebei domestic chicken. Res Vet Sci 92(1):76–79PubMedCrossRefGoogle Scholar
  11. Hayward WS, Neel BG, Astrin SM (1981) Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis. Nature 290(5806):475PubMedCrossRefGoogle Scholar
  12. Healy J, Tipton K (2007) Ceruloplasmin and what it might do. J Neural Trans 114(6):777CrossRefGoogle Scholar
  13. Jing YY, Li YS, Xin JK, Chai JQ (2014) Co-infection of ALV-J and Salmonella pullorum in laying hens. Pak Vet J 34(3):372–376Google Scholar
  14. Lau MC-C, Ng KY, Wong TL, Tong M, Lee TK, Ming X-Y, Law S, Lee NP, Cheung AL, Qin Y-R (2017) FSTL1 promotes metastasis and chemoresistance in esophageal squamous cell carcinoma through NFκB–BMP signaling cross-talk. Cancer Res 77(21):5886–5899PubMedCrossRefGoogle Scholar
  15. Le Luduec J, Condamine T, Louvet C, Thebault P, Heslan JM, Heslan M, Chiffoleau E, Cuturi MC (2008) An immunomodulatory role for follistatin-like 1 in heart allograft transplantation. Am J Trans 8(11):2297–2306CrossRefGoogle Scholar
  16. Li D, Wang Y, Xu N, Wei Q, Wu M, Li X, Zheng P, Sun S, Jin Y, Zhang G (2011) Follistatin-like protein 1 is elevated in systemic autoimmune diseases and correlated with disease activity in patients with rheumatoid arthritis. Arthritis Res Ther 13(1):R17PubMedPubMedCentralCrossRefGoogle Scholar
  17. Li J, Ding SC, Cho H, Chung BC, Gale M, Chanda SK, Diamond MS (2013) A short hairpin RNA screen of interferon-stimulated genes identifies a novel negative regulator of the cellular antiviral response. MBio 4(3):e00313–e00385Google Scholar
  18. Li X, Wang Q, Gao Y, Qi X, Wang Y, Gao H, Gao Y, Wang X (2015) Quantitative iTRAQ LC–MS/MS proteomics reveals the proteome profiles of DF-1 cells after infection with subgroup J Avian leukosis virus. Biomed Res Int.  https://doi.org/10.1155/2015/395307 PubMedPubMedCentralCrossRefGoogle Scholar
  19. Li H, Wang P, Lin L, Shi M, Gu Z, Huang T, Ml Mo, Wei T, Zhang H, Wei P (2019) The emergence of the infection of subgroup J avian leucosis virus escalated the tumour incidence in commercial Yellow chickens in Southern China in recent years. Transbound Emerg Dis 66(1):312–316PubMedCrossRefGoogle Scholar
  20. Lian L, Qu L, Zheng J, Liu C, Zhang Y, Chen Y, Xu G, Yang N (2010) Expression profiles of genes within a subregion of chicken major histocompatibility complex B in spleen after Marek’s disease virus infection. Poult Sci 89(10):2123–2129PubMedCrossRefGoogle Scholar
  21. Liu L, Yi J, Ray WK, Vu LT, Helm RF, Siegel PB, Cline MA, Gilbert ER (2019) Fasting differentially alters the hypothalamic proteome of chickens from lines with the propensity to be anorexic or obese. Nutr Diabetes 9(1):13PubMedPubMedCentralCrossRefGoogle Scholar
  22. Luo C, Chen M, Madden A, Xu H (2012) Expression of complement components and regulators by different subtypes of bone marrow-derived macrophages. Inflammation 35(4):1448–1461PubMedCrossRefGoogle Scholar
  23. Medzhitov R, Janeway CA (1997) Innate immunity: the virtues of a nonclonal system of recognition. Cell 91(3):295–298PubMedCrossRefGoogle Scholar
  24. Ouyang H, Wang Z, Chen X, Yu J, Li Z, Nie Q (2017) Proteomic analysis of chicken skeletal muscle during embryonic development. Front Physiol 8:281PubMedPubMedCentralCrossRefGoogle Scholar
  25. Payne L (1998) Retrovirus-induced disease in poultry. Poult Sci 77(8):1204–1212PubMedCrossRefGoogle Scholar
  26. Payne L, Nair V (2012) The long view: 40 years of avian leukosis research. Avian Pathol 41(1):11–19PubMedCrossRefGoogle Scholar
  27. Reddy SP, Britto R, Vinnakota K, Aparna H, Sreepathi HK, Thota B, Kumari A, Shilpa B, Vrinda M, Umesh S (2008) Novel glioblastoma markers with diagnostic and prognostic value identified through transcriptome analysis. Clin Cancer Res 14(10):2978–2987PubMedCrossRefGoogle Scholar
  28. Rowland AA, Chitwood PJ, Phillips MJ, Voeltz GK (2014) ER contact sites define the position and timing of endosome fission. Cell 159(5):1027–1041PubMedPubMedCentralCrossRefGoogle Scholar
  29. Rubin H, Vogt P (1962) An avian leukosis virus associated with stocks of Rous sarcoma virus. Virology 17(1):184–194PubMedCrossRefGoogle Scholar
  30. Serva A, Knapp B, Tsai Y-T, Claas C, Lisauskas T, Matula P, Harder N, Kaderali L, Rohr K, Erfle H (2012) miR-17-5p regulates endocytic trafficking through targeting TBC1D2/Armus. PLoS One 7(12):e52555PubMedPubMedCentralCrossRefGoogle Scholar
  31. Soll JM, Brickner JR, Mudge MC, Mosammaparast N (2018) RNA ligase-like domain in activating signal cointegrator 1 complex subunit 1 (ASCC1) regulates ASCC complex function during alkylation damage. J Biol Chem 293(35):13524–13533PubMedCrossRefGoogle Scholar
  32. Tanaka M, Ozaki S, Kawabata D, Kishimura M, Osakada F, Okubo M, Murakami M, Nakao K, Mimori T (2003) Potential preventive effects of follistatin-related protein/TSC-36 on joint destruction and antagonistic modulation of its autoantibodies in rheumatoid arthritis. Int Immunol 15(1):71–77PubMedCrossRefGoogle Scholar
  33. Trojan L, Schaaf A, Steidler A, Haak M, Thalmann G, Knoll T, Gretz N, Alken P, Michel MS (2005) Identification of metastasis-associated genes in prostate cancer by genetic profiling of human prostate cancer cell lines. Anticancer Res 25(1A):183–191PubMedGoogle Scholar
  34. Weissler KA, Caton AJ (2014) The role of T-cell receptor recognition of peptide: MHC complexes in the formation and activity of Foxp3+ regulatory T cells. Immunol Rev 259(1):11–22PubMedPubMedCentralCrossRefGoogle Scholar
  35. Yan X, Liang H, Deng T, Zhu K, Zhang S, Wang N, Jiang X, Wang X, Liu R, Zen K (2013) The identification of novel targets of miR-16 and characterization of their biological functions in cancer cells. Mol Cancer 12(1):92PubMedPubMedCentralCrossRefGoogle Scholar
  36. Yang Y, Zheng N, Zhao X, Zhang Y, Han R, Ma L, Zhao S, Li S, Guo T, Wang J (2015) Proteomic characterization and comparison of mammalian milk fat globule proteomes by iTRAQ analysis. J Proteom 116:34–43CrossRefGoogle Scholar
  37. Yang M, Song D, Cao X, Wu R, Liu B, Ye W, Wu J, Yue X (2017) Comparative proteomic analysis of milk-derived exosomes in human and bovine colostrum and mature milk samples by iTRAQ-coupled LC–MS/MS. Food Res Int 92:17–25PubMedCrossRefGoogle Scholar
  38. Zhang L, Jiang H, Xu G, Chu N, Xu N, Wen H, Gu B, Liu J, Mao S, Na R (2016) iTRAQ-based quantitative proteomic analysis reveals potential early diagnostic markers of clear-cell renal cell carcinoma. Biosci Trends 10(3):210–219PubMedCrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Fei Ye
    • 1
    • 2
  • Qijian He
    • 1
  • Yan Wang
    • 1
  • Can Cui
    • 1
  • Fuling Yang
    • 1
  • Bin Luo
    • 1
  • Huadong Yin
    • 1
  • Xiaoling Zhao
    • 1
  • Diyan Li
    • 1
  • Hengyong Xu
    • 1
  • Hua Li
    • 1
    • 2
    Email author
  • Qing Zhu
    • 1
    Email author
  1. 1.Institute of Animal Genetics and BreedingSichuan Agricultural UniversityChengduChina
  2. 2.Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise BreedingFoshan UniversityFoshanChina

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