Advertisement

3 Biotech

, 9:313 | Cite as

MSAP analysis of epigenetic changes reveals the mechanism of bicolor petal formation in Paeonia suffruticosa ‘Shima Nishiki’

  • Yu Wang
  • Mingyuan Zhao
  • Zongda Xu
  • Shuai Qi
  • Xiaoyan YuEmail author
  • Xu HanEmail author
Original Article
  • 20 Downloads

Abstract

Paeonia suffruticosa ‘Shima Nishiki’ is a very valuable bicolor cultivar because of its distinctive and colorful flowers. However, our understanding of the mechanisms underlying bicolor petal formation is limited. In this study, we used the methylation-sensitive amplified polymorphism (MSAP) method to assess the levels and pattern of cytosine methylation in different-colored petals during floral development. Our data showed differences in the methylation levels of red and pink petals. The methylation rate of the red petals was consistently higher than that of the pink petals, with maximum values of 58.45% (red petals) and 44.36% (pink petals) during the S2 developmental stage. However, obvious differences were not observed in the patterns of cytosine methylation in different-colored petals; methylation and demethylation occurred simultaneously and the proportions were similar. In addition, we isolated and sequenced the differentially methylated fragments and found that one fragment was homologous to the bHLH1 gene of P. suffruticosa ‘Luoyang Hong’; its expression pattern suggested that the bHLH1 gene may be involved in the regulation of the formation of bicolor flowers in P. suffruticosa ‘Shima Nishiki’. These results will provide a valuable resource for further investigation of the genetic mechanisms underlying bicolor petal formation in P. suffruticosa ‘Shima Nishiki’.

Keywords

P. suffruticosa ‘Shima Nishiki’ Bicolor DNA methylation Methylation-sensitive amplification polymorphism 

Notes

Acknowledgments

This project was funded by the National Science Foundation of China (NSFC) (31700622).

Author contributions

XY and XH conceived the idea and supervised the project. ZX and YW participated in the design of the study and in interpreting the data, and manuscript preparation. YW performed the experiments and the data analysis and drafted the manuscript. SQ contributed analysis tools and participated in the data analysis. All authors carefully read and approved the final version of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

13205_2019_1844_MOESM1_ESM.doc (71 kb)
Supplementary material 1 (DOC 71 kb)

References

  1. Banks JA, Masson P, Fedoroff N (1998) Molecular mechanisms in the developmental regulation of the maize suppressor-mutator transposable element. Genes Dev 2:1364–1380CrossRefGoogle Scholar
  2. Chandler VL, Walbot V (1986) DNA modification of a maize transposable element correlates with loss of activity. Proc Natl Acad Sci USA 83:1767–1771CrossRefGoogle Scholar
  3. Cheng C, Daigen M, Hirochika H (2006) Epigenetic regulation of the rice retrotransposon. Tos17 Mol Gen Genom 276:378–390CrossRefGoogle Scholar
  4. Cocciolone S, Cone K (1993) Pl-Bh, an anthocyanin regulatory gene of maize that leads to variegated pigmentation. Genetics 135:575–588PubMedPubMedCentralGoogle Scholar
  5. Du H, Wu J, Ji KX, Zeng QY, Wang LS (2015) Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in paeonia. J Exp Bot 66:6563–6577CrossRefGoogle Scholar
  6. Fu H, Zhang YX, Liu CY, Gai WL, Zhan XM, Gai SP (2017) Cloning and expression analysis in tree Peony of DNA methyl binding domain protein gene PsMBD5. North China Agric J 32:66–70 (in chinese) Google Scholar
  7. Garcia RN, D’vila MF, Robe LJ (2007) First evidence of methylation in the genome of Drosophila willistoni. Genetica 131:91–105CrossRefGoogle Scholar
  8. Guo WW, Dong L, Wang LY, Chen RX, Liu AQ (2004) The postharvest characteristics and water balance of some cultivars of tree-peony cut flowers. Sci Silvae Sin 40:89–93 (in chinese) Google Scholar
  9. Han ZY (2015) Study on McCOP1 promoter methylated by RdDM pathway to regulate anthocyanin biosynthesis in Crabapple (Maius cv. spp). Beijing Agricultural College, Beijing (in chinese) Google Scholar
  10. Hichri I, Barrieu F, Bogs J, Kappel C, Delrot S, Lauvergeat V (2011) Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway. J Exp Bot 62:2465–2483CrossRefGoogle Scholar
  11. Iwasaki M, Paszkowski J (2014) Epigenetic memory in plants. EMBO J 33:1987–1998CrossRefGoogle Scholar
  12. Ji LJ, Wang Q, da Silva JAT, Yu XN (2012) The genetic diversity of Paeonia L. Sci Hortic 143:62–74CrossRefGoogle Scholar
  13. Layne DR, Bassi D (2008) The peach: Botany, production and uses. CAB International, WallingfordCrossRefGoogle Scholar
  14. Li MT, Yu LJ, Wang LM, Liu JM, Lei CH (2005) Chrysanthemum flower color inheritance and flower color chimera discovery. Genetics 27:948–952 (in chinese) Google Scholar
  15. Li WG, Chang TJ, Gong HM (2008) MSAP technology and its application in plant genetics research. Biotechnology 18:83–87 (in chinese) Google Scholar
  16. Lim SH, Kim JK, Kim DH, Sohn SH, Lee JY, Kim YM, Ha SH (2011) Flower color modification by manipulating flavonoid biosynthetic pathway. Kor J Hort Sci Technol 29:511–522Google Scholar
  17. Liu XJ, Chuang YN, Chiou CY, Chin DC, Shen FQ, Yeh KW (2012) Methylation effect on chalcone synthase gene expression determines anthocyanin pigmentation in floral tissues of two oncidium orchid cultivars. Planta 236:401–409CrossRefGoogle Scholar
  18. Lu GY, Wu XM, Chen BY, Gao GZ, Xu K, Li XZ (2006) Detection of DNA methylation changes during seed germination in rapeseed (Brassica napus). Chin Sci Bull 51:182–190CrossRefGoogle Scholar
  19. Murray MG, Thompson WF (1980) Rapid isolation of high-weight plant DNA. Nucleic Acids Res 8:4231–4235CrossRefGoogle Scholar
  20. Noman A, Aqeel M, Deng JM, Khalid N, Sanaullah T, Shuilin H (2017) Biotechnological advancements for improving floral attributes in ornamental plants. Front Plant Sci. 8:530CrossRefGoogle Scholar
  21. Orłowska R, Machczyńska J, Oleszczuk S, Zimny J, Bednarek PT (2016) DNAmethylation changes and TE activity induced in tissue cultures of barley (Hordeum vulgare L.). J Biol Res (Thessaloniki) 23:19CrossRefGoogle Scholar
  22. Peredo EL, Revilla M, Arroyo-Garcí R (2006) Assessment of genetic and epigenetic variation in hop plants regenerated f rom sequential subcultures of organogenic calli. Plant Physiol 163:1071–1079CrossRefGoogle Scholar
  23. Portis E, Acquadro A, Comino C, Lanteri Sl (2004) Analysis of DNA methylation during germination of peper (Capsicum annuum L.) seeds using methylation-sensitive amplification polymorphism (MSAP). Plant Sci 166:169–178CrossRefGoogle Scholar
  24. Saze H, Tsugane K, Kanno T, Nishimura T (2012) DNA methylation in plants: relationship to small RNAs and histone modifications, and functions in transposon inactivation. Plant Cell Physiol 53:766–784CrossRefGoogle Scholar
  25. Sekhon R, Chopra S (2009) Progressive loss of DNA methylation releases epigenetic gene silencing from a tandemly repeated maize MYB Gene. Genetics 181:81–91CrossRefGoogle Scholar
  26. Tanaka Y, Tsuda S, Kusumi T (1998) Metabolic engineering to modify flower color. Plant Cell Physiol 39:1119–1126CrossRefGoogle Scholar
  27. Telias A, Lin-wang K, Stevenson DE, Cooney JM, Bradeen JM (2011) Apple skin patterning is associated with differential expression of MYB10. BMC Plant Biol 11:1–15CrossRefGoogle Scholar
  28. Wang ZG, Meng D, Wang AD, Li T, Jiang S, Cong P, Li T (2013) The methylation of the PcMYB10 promoter is associated with green-skinned sport in Max Red Bartlett pear. Plant Physiol 162:885–896CrossRefGoogle Scholar
  29. Yan JF (2014) Study on pigment metabolism and mutation mechanism of chrysanthemum flower color chimera. Suzhou University, Suzhou (in chinese) Google Scholar
  30. Zhang YX, Zhan L, Gai SP, Liu CY, Lu S (2015) Cloning and expression analysis of the R2R3- PsMYB1 gene associated with bud dormancy during chilling treatment in the tree peony (Paeonia suffruticosa). Plant Growth Regul 75:667–676CrossRefGoogle Scholar
  31. Zhang XP, Zhao MYGJ, Zhao LY, Xu ZD (2018) Anatomical and biochemical analyses reveal the mechanism of double-color formation in Paeonia suffruticosa ‘Shima Nishiki’. Biotech 8:420–429Google Scholar
  32. Zhao DQ, Tao J (2015) Recent advances on the development and regulation of flower color in ornamental plants. Front Plant Sci 6:261PubMedPubMedCentralGoogle Scholar
  33. Zhao XX, Chai Y, Liu B (2007) Epigenetic inheritance and variation of DNA methylation level and pat tern in maize intra-specif ic hybrids. Plant Sci 172:930–938CrossRefGoogle Scholar
  34. Zhao Y, Yu S, Xing C, Fan S, Song M (2008) Analysis of DNA methylation in cotton hybrids and their parents. Mol Biol 42:169–178CrossRefGoogle Scholar
  35. Zilberman D, Gehring M, Tran RK, Ballinger T, Henikoff S (2006) Genome-wide analysis of Arabidopsis thaliana DNA methylation uncovers an interdependence between methylation and transcription. Nat Genet 39:61–69CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.College of ForestryShandong Agricultural UniversityTaianChina

Personalised recommendations