Amelioration of antioxidant potential, toxicity, and antihyperglycemic activity of Hippophae salicifolia D. Don leaf extracts in alloxan-induced diabetic rats
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Efficacy of several plant extracts in the clinical research for modulating oxidative stress correlated with diabetes mellitus (DM) is well documented. In the present study, we investigated the in vitro antioxidant activity, toxicity, and anti-diabetic activity of methanolic extract of Hippophae salicifolia leaves in normal and alloxan-induced diabetic wistar rats. H. salicifolia leaves were found to be rich in antioxidants. The acute toxicity test of methanolic extract of H. salicifolia leaves revealed that the median lethal dose (LD50) was found to be 3.92 g/kg body weight in mice. Administration of H. salicifolia leaves at 200 mg/kg and 400 mg/kg in alloxan-induced diabetic rats illustrated significant reduction (22% and 39%, respectively) in fasting blood glucose compared to diabetic control. Both the doses were found to be effective when compared to diabetic rats. The Hippophae-treated diabetic rats showed significant increase in the endogenous antioxidant enzymes, superoxide dismutase (50% and 74%, respectively), glutathione peroxidase (57% and 41%, respectively) and decrease in malondialdehyde (33% and 15%, respectively) levels. These results suggested that the methanolic leaf extract of H. salicifolia enhanced the antioxidant defence against reactive oxygen species produced under hyperglycaemic conditions.
KeywordsHippophae salicifolia Antioxidants Diabetes Toxicology Flavonoids Polyphenols Sea buckthorn
Authors are grateful to Biotechnology-Skill Enhancement program, under Govt. of Karnataka at Maharani Lakshmi Ammanni College for Women, for funding animal house facility to carry out this study.
TU and AKG performed the experiments, analyzed the data and prepared the manuscript. BCB procured and prepared the sample and wrote a part of the manuscript. SKM conceived the idea, designed and coordinated the experiments, improved the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in the studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.
- Ahmed D, Kumar V, Verma A, Gupta PS, Kumar H, Dhingra V, Mishra V, Sharma M (2014) Anti-diabetic, renal/hepatic/pancreas/cardiac protective and antioxidant potential of methanol/dichloromethane extract of Albizzia Lebbeck Benth. stem bark (ALEx) on streptozotocin induced diabetic rats. BMC Complement Altern Med 14:243CrossRefGoogle Scholar
- Cao QH, Qu WJ, Deng YX, Zhang ZC, Niu W, Pan YF (2003) Effects of flavonoids from the seed and fruit residue of Hippophae rhamnoides L. on glycometabolism in mice. J Chin Med Mater 26:735–737Google Scholar
- Chang CLT, Lin Y, Bartolome AP, Chen YC, Chiu SC, Yang WC (2013) Herbal therapies for type 2 diabetes mellitus: chemistry, biology, and potential application of selected plants and compounds. Evid-Based Complement Altern Med 2013:378657Google Scholar
- Loomis TA, Hayes AW (1996) Loomis’s essentials of toxicology. Academic press, California, pp 208–245Google Scholar
- McLellan KCP, Wyne K, Villagomez ET, Hsueh WA (2014) Therapeutic interventions to reduce the risk of progression from prediabetes to type 2 diabetes mellitus. Ther. Clin. Risk Manag. 10:173–188Google Scholar
- Middha SK, Usha T, Pande V (2016) Insights into the causes and anti-hyperglycemic effects of Punica granatum rind in alloxan-induced diabetic rats. Chiang Mai J Sci 43:112–122Google Scholar
- Perumal SR, Ponnampalam G (2007) current status of herbal and their future perspectives. Nature Preced 10101(1176):1Google Scholar
- Rawat S, Kumar N, Kothiyal P (2013) Evaluate the anti-diabetic activity of Myrica esculenta in streptozotocin induced rats. Int J Univ Pharm Bio Sci 2(6):510–525Google Scholar
- Saikia M, Handique PJ (2013) Antioxidant and antibacterial activity of leaf, bark, pulp and seed extracts of seabuckthorn (Hippophae salicifolia D. Don) of Sikkim Himalayas. J Med Plants Res 7(19):1330–1338Google Scholar
- Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16:144–158Google Scholar
- Wang B, Lin L, Ni Q, Su CL (2011) Hippophae rhamnoides Linn. for treatment of diabetes mellitus: a review. J Med Plant Res 5(13):2599–2607Google Scholar