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3 Biotech

, 9:296 | Cite as

Molecular characterization and an 80-bp indel polymorphism within the prolactin receptor (PRLR) gene and its associations with chicken growth and carcass traits

  • Ke Liang
  • Xiangnan Wang
  • Xiaoxiao Tian
  • Rui Geng
  • Wenya Li
  • Zhenzhu Jing
  • Ruili Han
  • Yadong Tian
  • Xiaojun Liu
  • Xiangtao Kang
  • Zhuanjian LiEmail author
Original Article

Abstract

The prolactin receptor (PRLR), a type I cytokine receptor, must bind prolactin (PRL) to act on target cells to mediate various physiological functions, including reproduction and lactation. This study identified an 80-bp insertion/deletion (indel) polymorphism in the 3′-untranslated region (3′-UTR) of the chicken PRLR gene in 3736 individuals from 15 breeds and analyzed its associations with growth and carcass traits in an F2 resource population. The results of the association analysis indicated that the 80-bp indel polymorphism was significantly (P < 0.05) or very significantly (P < 0.01) associated with multiple growth and carcass traits, such as body weight, leg weight, and shank length. In addition, we found that during the breeding process of commercial laying hens and commercial broilers, the 80-bp indel locus was artificially selected for the II genotype. Together, our findings reveal that this 80-bp indel polymorphism has potential as a new molecular marker for marker-assisted selection of chicken growth and carcass traits.

Keywords

Prolactin receptor Association analysis Growth traits Insertion/deletion Genetic differentiation 

Notes

Acknowledgments

This study was funded by the NSFC-Henan Joint Grant (U1804107), the Key Science and Technology Research Project of Henan Province (151100110800), the National Natural Science Foundation of China (31572356), and the Program for Innovation Research Team of the Ministry of Education (IRT16R23).

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there are no conflicts of interest.

Supplementary material

13205_2019_1827_MOESM1_ESM.docx (791 kb)
Supplementary material 1 (DOCX 791 kb) Table S1 mRNA and amino acid information of the PRLR gene from 16 species. Table S2 Websites and purposes for bioinformatic analyses. Fig. S1 Hydrophilicity and hydrophobicity analyses. Fig. S2 Signal peptide prediction. Fig. S3 Transmembrane domain prediction. Fig. S4 Phosphorylation site prediction. Fig. S5 Secondary structure prediction. Note: Blue represents α helix, green represents β turn, red represents extended strand, and orange represents random coil.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.College of Animal Science and Veterinary MedicineHenan Agricultural UniversityZhengzhouChina

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