3 Biotech

, 9:187 | Cite as

Prospecting prebiotics, innovative evaluation methods, and their health applications: a review

  • Ishu Khangwal
  • Pratyoosh ShuklaEmail author
Review Article


Prebiotics are necessary natural and synthetic food ingredients that help in the growth and development of gut microflora. There is a complex relationship between gut dysbiosis and microbes, so alteration in both probiotics and prebiotics can reduce illness of gut, which further plays a decisive role in human health. The prebiotic efficiency can be validated using various in vitro and in vivo experiments, and this gives an important insight to this field. This review focuses on these aspects including the standardized assessment of prebiotics and its metabolic products for customary applications. This review has also summarized the mechanism of their beneficial actions such as immunomodulation, nutrient absorption, pathogen inhibition, etc., and its significance in human nutrition. In addition to this, some fascinating applications of prebiotics in health-related disorders have also discussed, with current challenges in this facet.


Prebiotics Gut microbiota Gastrointestinal tract Oligosaccharides Human health 



The authors acknowledge Maharshi Dayanand University, Rohtak, India, for providing infrastructure facility. IK acknowledges the support as University Research Scholarship by M.D. University, Rohtak, India (Grant No. R&S/R-15/19/1868). PS acknowledges the infrastructural support from Department of Science and Technology, New Delhi, Govt. of India, FIST grant (Grant No. 1196 SR/FST/LS-I/ 2017/4) and Department of Biotechnology, Government of India (Grant No. BT/PR27437/BCE/8/1433/2018). PS also acknowledges Department of Microbiology, Barkatullah University, Bhopal, India, for their infrastructural support for D.Sc. work.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Enzyme Technology and Protein Bioinformatics Laboratory, Department of MicrobiologyMaharshi Dayanand UniversityRohtakIndia

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