Matrix attachment regions as a tool to influence plant transgene expression
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The inclusion of special regulatory sequences known as matrix attachment regions (MARs) in transgene constructs has been suggested as a possible approach to stabilise the expression of foreign heterological genes. The present review provides a brief summary regarding the MARs that have been used in investigations studying their influence on plant transgene expression in different plants with different promoters and reporter genes, and the comparison of these investigations.
KeywordsTransgenic plant Matrix attachment regions MAR Transgene expression
This study was supported by the Russian Science Foundation Grant #17-75-10093.
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Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
- Able JA, Rathus CO, Carroll BJ, Godwin ID (2004) Enhancing transgene expression levels in sorghum: current status and future goals. In: Seetharama N, Godwin ID (eds) Sorghum tissue culture and transformation. Oxford Publishers, New Delhi, pp 85–96Google Scholar
- De Bolle MFC, Butaye KMJ, Coucke WJW, Goderis IJWM, Wouters PFJ, van Boxel N, Broekaert WF, Cammue BPA (2003) Analysis of the influence of promoter elements and a matrix attachment region on the inter-individual variation of transgene expression in populations of Arabidopsis thaliana. Plant Sci 165:169–179CrossRefGoogle Scholar
- Ji L, Xu R, Lu L, Zhang J, Yang G, Huang J, Wu C, Zheng C (2013) TM6, a novel nuclear matrix attachment region, enhances its flanking gene expression through influencing their chromatin structure. Mol Cells 36(2):127–137. https://doi.org/10.1007/s10059-013-0092-z CrossRefPubMedPubMedCentralGoogle Scholar
- Levin JS, Thompson WF, Csinos AS, Stephenson MG, Weissinger AK (2005) Matrix attachment regions increase the efficiency and stability of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco. Transgenic Res 14:193–206. https://doi.org/10.1007/s11248-004-5413-8 CrossRefPubMedGoogle Scholar
- Odell JT and Krebbers E (1998). Enhanced transgene expression in a population of monocot cells employing scaffold attachment regions. World Patent OfficeGoogle Scholar
- Tetko IV, Haberer G, Rudd S, Meyers B, Mewes H-W, Mayer KFX (2006) Spatiotemporal expression control correlates with intragenic scaffold matrix attachment regions (S/MARs) in Arabidopsis thaliana. PLoS Comput Biol 2(3):e21. https://doi.org/10.1371/journal.pcbi.0020021 CrossRefPubMedPubMedCentralGoogle Scholar
- Torney F, Partier A, Says-Lesage V, Nadaud I, Barret P, Beckert M (2004) Heritable transgene expression pattern imposed onto maize ubiquitin promoter by maize adh-1 matrix attachment regions: tissue and developmental specificity in maize transgenic plants. Plant Cell Rep 22(12):931–938CrossRefGoogle Scholar
- van der Geest AHY, Hall GE Jr, Spiker S, Hall TC (1994) The β-phaseolin gene is flanked by matrix attachment regions. Plant Mol Biol 6:413–423Google Scholar