Development and utilization of gyrA and gyrB gene-based diagnostics for the phytoplasma classified under 16Sr I group in plants and insects
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In the present study, a new set of primers of gyrA and gyrB genes of the phytoplasma genome were designed and validated for the successful detection and taxonomic classification of the previously identified phytoplasma strains of ‘Candidatus P. asteris’ (16SrI-B subgroup) associated with Catharanthus leaf yellows, sesame phyllody and the leafhopper (Hishimonus phycitis). Our results suggested the ability and sensitivity of gyrA and gyrB genes as an alternative molecular marker to identify the Ca. P. asteris strain up to subgroup level associated both with plants and insects.
KeywordsMultilocus genes Validation Phytoplasma detection Leafhopper
The authors wish to express sincere thanks to the Head, Division of Plant Pathology and the Director, Indian Agricultural Research Institute for providing laboratory facilities. Funding was provided by Department of Biotechnology, Ministry of Science and Technology (Grant no: 24/468).
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Conflict of interest
The authors declare that they have no conflict of interest.
- Hodgetts J, Dickinson M (2010) Phytoplasma phylogeny and detection based on genes other than 16S rRNA. In: Weintraub PG, Jones P (eds) Phytoplasmas genomes, plant hosts and vectors. CAB International, Wallingford, pp 93–113Google Scholar
- Langer M, Maixner M (2004) Molecular characterization of grapevine yellows associated phytoplasmas of the stolbur group based on RFLP-analysis of non-ribosomal DNA. Vitis 43:191–200Google Scholar
- Lee IM, Zhao Y, Davis RE, Wei W, Martini M (2007) Prospects of DNA-based systems for differentiation and classification of phytoplasmas. Bull Insectol 60(2):239–244Google Scholar
- Madhupriya (2016) Molecular characterization of phytoplasmas associated with important ornamental plant species in Northern India. Ph.D. Thesis, Amity University, Haryana, p 293Google Scholar
- Yu S, Li Y, Ren Z, Song C, Lin C, Piao C, Tian G (2017) Multilocus sequence analysis for revealing finer genetic variation and phylogenetic interrelatedness of phytoplasma strains in 16SrI group in China. Sci Silv Sin 53(3):105–118Google Scholar