Assessing oil accumulation in the oleaginous yeast Cystobasidium oligophagum JRC1 using dairy waste cheese whey as a substrate
This study assesses the potential for the lipid production by the oleaginous yeast Cystobasidium oligophagum JRC1 using dairy industry waste cheese whey as a substrate. Cheese whey was used either untreated (UCW) or deproteinized (DCW) at different concentrations (25–100%) to serve as the carbon and energy source. Both UCW and DCW supported high biomass and lipid productivities. The biomass productivity of 0.076 ± 0.0004 and 0.124 ± 0.0021 g/L h, lipid productivity of 0.0335 ± 0.0004 and 0.0272 ± 0.0008 g/L h, and the lipid content of 44.12 ± 0.84 and 21.79 ± 1.00% were achieved for 100% DCW and UCW, respectively. The soluble chemical oxygen demand (sCOD) removal rate was 8.049 ± 0.198 and 10.61 ± 0.0165 g/L day (84.91 ± 0.155 and 86.82 ± 0.067% removal) for 100% DCW and UCW, respectively. Fatty acid methyl ester (FAME) composition obtained using GC-FID studies revealed the presence of C16 and C18 fatty acid in the lipid extract and the biodiesel properties were found to be in accordance with ASTM and EN standards. The study presents a method for the valorization of cheese whey waste into a feasible feedstock for biodiesel.
KeywordsCheese whey Deproteinized cheese whey Biodiesel Oleaginous yeast Rhodotorula Cystobasidium oligophagum
Author MC thanks IIT Jodhpur for providing various research facilities. Author SV thanks the Ministry of Human Resource Development (MHRD) India, for his senior research fellowship (SRF). Authors thank Dr. Mahesh Balwant Khot (SERB, National Postdoctoral Fellow, Biofuels division, CSIR-Indian Institute of Petroleum, Dehradun, India) for GC-FID analysis.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest in the publication.
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