3 Biotech

, 9:160 | Cite as

Molecular characterization of laccase genes from the basidiomycete Trametes hirsuta Bm-2 and analysis of the 5′ untranslated region (5′UTR)

  • Alejandrina Pereira-Patrón
  • Sara Solis-Pereira
  • Gabriel Lizama-Uc
  • Jorge H. Ramírez-Prado
  • Daisy Pérez-Brito
  • Raul Tapia-TussellEmail author
Original Article


The aim of this study was to identify and characterize laccase genes produced by Trametes hirsuta Bm-2 in a liquid medium, both with and without induction. The amplification of 5′and 3′regions of laccase sequences was obtained by the RACE-PCR method, and these were assembled to obtain a cDNA of total length. Two new laccase genes were isolated from basal medium (lac-B) and lignocellulosic grapefruit substrate (lac-T), both encoding open reading frames of 2566 bp. Both laccase-predicted proteins consisted of 521 amino acids, four copper-binding regions, a signal peptide, and five potential glycosilation sites (Asn-Xaa-Ser/Tre). Moreover, the deduced amino acid sequences share about 76–85% identity with other laccases of WRF. Sequence comparison showed 47 synonymous point mutations between lac-B and lac-T. In addition, 5′ untranslated regions (UTR) of laccase genes lac-B and lac-T showed differences in length and number of regulatory elements that may affect transcriptional or translational expression of these genes.


Trametes hirsuta Laccase genes RACE-PCR Untranslated regions 



The authors wish to express their gratitude to National Science and Technology Council, Mexico (CONACYT) for providing the financial support for this research (Project No. 248295).

Author contributions

All the authors contributed to this work. Tapia-Tussell and Solis-Pereira conceived, designed and wrote the paper; Pereira-Patron performed the experiments and analyzed the data; Lizama-Uc, Perez-Brito and Ramirez-Prado participated in the data analysis of untranslated region and writing of the paper. All authors reviewed and approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest regarding publication of this paper.


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Depto. de Ingeniería Química y Bioquímica, Tecnológico Nacional de México, Instituto Tecnológico de MéridaMéridaMexico
  2. 2.Unidad de Biotecnología, Centro de Investigación Científica de YucatánMéridaMexico
  3. 3.Laboratorio GeMBioCentro de Investigación Científica de YucatánMéridaMexico
  4. 4.Unidad de Energía Renovable, Centro de Investigación Científica de YucatánMéridaMexico

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