3 Biotech

, 9:154 | Cite as

Elimination of Bean yellow mosaic virus from infected cormels of three cultivars of gladiolus using thermo-, electro- and chemotherapy

  • Charanjeet Kaur
  • Rashmi Raj
  • Susheel Kumar
  • D. K. Purshottam
  • Lalit Agrawal
  • Puneet S. Chauhan
  • S. K. RajEmail author
Original Article


Bean yellow mosaic virus (BYMV) is a prevalent virus and major threat to gladiolus cultivation the world over. In the gladiolus repository at CSIR-NBRI, Lucknow, several plants (82–88%) of three economically important cultivars were found infected by BYMV showing severe mosaic and stripe symptoms. Affected plants exhibit diminished quality and quantity of florets and corms, thus reducing their value. Attempts were made to eliminate BYMV from the infected gladiolus cormel explants in vitro through thermotherapy (37 °C for 30 days), chemotherapy (30 mg/L ribavirin for 30 days), and electrotherapy (30 mA for 20 min), either alone and in different combinations. The in vitro regenerated plants were free from BYMV infection when checked by RT-PCR using BYMV-specific primers. The combination of electro- and chemotherapies has given the best response as compared to other treatments. Among the individual therapies, electrotherapy (30 mA/20 min) was found to be the best for and production of BYMV-free gladiolus plants (44–46%) with moderate regeneration efficiency (54–58%) followed by chemotherapy and thermotherapy. However, the cormels obtained from a combination of electro- and chemotherapy treatment (30 mA/20 min + 30 mg/L) has given highest virus free (46–52%) and highest therapy efficiency indices (56%) as compared to other treatments. Further, these cormels showed better developed root systems and produced more cormels which were larger in size as compared to the other treatments and control when grown in tissue culture media.


Gladiolus BYMV Chemotherapy Electrotherapy Cormels Virus free 



Thanks are due to the director CSIR-NBRI, Lucknow for facilities and Dr. R. K. Roy, Head Floriculture, CSIR-NBRI, Lucknow for providing gladiolus cultivars. The authors C. Kaur and Rashmi Raj are thankful to University Grants Commission, India for fellowship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13205_2019_1684_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 KB)


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Plant Molecular Virology Laboratory, CPMBCSIR-National Botanical Research InstituteLucknowIndia
  2. 2.Plant Tissue Culture Laboratory, CPMBCSIR-National Botanical Research InstituteLucknowIndia
  3. 3.Division of Plant Microbe InteractionCSIR-National Botanical Research InstituteLucknowIndia
  4. 4.AcSIR (Academy of Scientific and Innovative Research)New DelhiIndia

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