3 Biotech

, 9:144 | Cite as

Diversity, community distribution and growth promotion activities of endophytes associated with halophyte Lycium ruthenicum Murr

  • Yong-Hong Liu
  • Yong-Yang Wei
  • Osama Abdalla Abdelshafy Mohamad
  • Nimaichand Salam
  • Yong-guang Zhang
  • Jian-Wei Guo
  • Li Li
  • Dilfuza Egamberdieva
  • Wen-Jun LiEmail author
Original Article


The purpose of this study was to investigate the composition, diversity, distribution, and growth promotion activity of endophytic bacteria isolated from L. ruthenicum Murr. Consequently, a total of 109 endophytic bacteria affiliated to 3 phyla, 12 orders and 36 genera were isolated using nine different selective media, from which, Actinobacteria was the dominant taxon containing seven orders at the phylum level; Micrococcales showed the highest diversity containing 12 genera at the family level. Based on PAST and SPSS analysis, species diversity and abundance were mostly isolated from nutritious soil condition (22 genera) and root tissue (27 genera). Furthermore, growth phase showed significant effect on the endophytic bacteria community (28 genera at dormancy and 17 genera at fluorescence stage). With regard to ex situ plant growth-promoting activities, Streptomyces dominated and exhibited broad ability in terms of their potential to grow on nitrogen-free media, synthesize cellulase and lipase enzymes. Characterization of potential plant-beneficial traits indicate that endophytic bacteria exhibited a number of positive activities, including potential diazotrophy (n = 66), phosphate-solubilizing (n = 6), production of lipase (n = 21) and cellulose (n = 35). Two strains, representing Bacillus sp. EGI 63071 and EGI 63106, were found to be effective in promoting the growth of Triticum aestivum (wheat: Xindong No.18) seedling under salt stress conditions.


Environmental microbiology Endophytes Diversity Halophyte Lycium ruthenicum Growth promotion 



This research was supported by Xinjiang Uygur Autonomous Region regional coordinated innovation project (Shanghai cooperation organization science and technology partnership program) (No. 2017E01031) and China Biodiversity Observation Networks (Sino BON). This research was supported by Chinese Academy of Sciences President’s International Fellowship Initiative (Grant No. 2018VBA002S) for Dilfuza Egamberdieva.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

13205_2019_1678_MOESM1_ESM.docx (31 kb)
Supplementary material 1 (DOCX 30 KB)


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Yong-Hong Liu
    • 1
    • 4
  • Yong-Yang Wei
    • 1
  • Osama Abdalla Abdelshafy Mohamad
    • 1
    • 5
  • Nimaichand Salam
    • 2
  • Yong-guang Zhang
    • 1
  • Jian-Wei Guo
    • 1
    • 3
  • Li Li
    • 1
  • Dilfuza Egamberdieva
    • 1
    • 6
  • Wen-Jun Li
    • 1
    • 2
    Email author
  1. 1.Key Laboratory of Biogeography and Bioresource in Arid LandXinjiang Institute of Ecology and Geography, Chinese Academy of SciencesUrumqiPeople’s Republic of China
  2. 2.State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life SciencesSun Yat-Sen UniversityGuangzhouPeople’s Republic of China
  3. 3.Key Laboratory of Crops with High Quality and Efficient Cultivation and Security Control, Yunnan Higher Education InstitutionsHonghe UniversityMengziPeople’s Republic of China
  4. 4.University of Chinese Academy of SciencesBeijingPeople’s Republic of China
  5. 5.Department of Biological, Marine Sciences, and Environmental Agriculture, Institute for Post Graduate Environmental StudiesArish UniversityArishEgypt
  6. 6.Department of Biotechnology and Microbiology, Faculty of Biology and Soil ScienceNational University of UzbekistanTashkentUzbekistan

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