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3 Biotech

, 9:145 | Cite as

In vitro growth of Physalis peruviana L. affected by silver nanoparticles

  • Caroline de Oliveira TimoteoEmail author
  • Renato Paiva
  • Michele Valquíria dos Reis
  • Pedro Ivo Cunha Claro
  • Luthiane Machado Ferraz
  • Jose Manoel Marconcini
  • Juliano Elvis de Oliveira
Original Article
  • 33 Downloads

Abstract

The effect of silver nanoparticles (AgNPs) on plant cells, since their phytotoxicity potential is not yet fully understood. In this context, the aim of the present study was to elucidate the effects of AgNPs in the in vitro culture of Physalis peruviana. For this purpose, P. peruviana seeds were grown in MS medium supplemented with different concentrations of AgNPs. Growth and development of seedlings were evaluated through germination, seedling size and biomass and biochemical and anatomical analyses. At the end of 60 days of cultivation, it was observed that the in vitro germination of this species is not affected by the presence of AgNPs and that at low concentrations (0.385 mg L−1) it can promote an increase in seedlings biomass. However, higher concentration (15.4 mg L−1) leads to a reduction in seedling size and root system, but no changes were observed in the seedlings antioxidant metabolism and anatomy. These results demonstrate that the phytotoxicity of AgNPs in P. peruviana is related to the concentration of nanoparticles to which the specie is exposed.

Keywords

AgNPs Nanotechnology Phytotoxicity Tissue culture Solanaceae 

Notes

Acknowledgements

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. Sincere thanks to the Foundation for Research Support of the State of Minas Gerais (FAPEMIG) and the National Council for Scientific and Technological Development (CNPq), for financial support. We are also grateful to the National Laboratory of Nanotechnology for Agribusiness—Embrapa Instrumentation—São Carlos-SP, where part of the experiment was performed.

Compliance with ethical standards

Conflict of interest

The authors state that the present study was conducted in the absence of commercial or financial relationships that could result in a potential conflict of interest.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Caroline de Oliveira Timoteo
    • 1
    Email author
  • Renato Paiva
    • 1
  • Michele Valquíria dos Reis
    • 1
  • Pedro Ivo Cunha Claro
    • 2
  • Luthiane Machado Ferraz
    • 3
  • Jose Manoel Marconcini
    • 4
  • Juliano Elvis de Oliveira
    • 3
  1. 1.Department of Biology, Sector of Plant physiologyFederal University of LavrasLavrasBrazil
  2. 2.Graduate Program in Materials Science and Engineering (PPG-CEM)Federal University of São CarlosSão CarlosBrazil
  3. 3.Department of EngineeringFederal University of LavrasLavrasBrazil
  4. 4.National Nanotechnology Laboratory for Agribusiness (LNNA), Embrapa InstrumentationSão CarlosBrazil

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