Characterization, solubility and antibacterial activity of inclusion complex of questin with hydroxypropyl-β-cyclodextrin
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To increase the water solubility of questin and broaden its application in preventing and treating Vibrio diseases in aquaculture, an inclusion complex of questin with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared by stirring and coevaporation strategy. The results of thin-layer chromatography and nuclear magnetic resonance spectrum confirmed the inclusion of questin into HP-β-CD. The aqueous solubility of questin in the inclusion complex reached (62.63 ± 1.21) µg/mL, which was 110 times of questin’s original solubility. The preliminary agar diffusion method indicated that questin–HP-β-CD inclusion complex showed enhanced antibacterial activity against Vibrio harveyi compared with free questin. This finding provided a reliable basis for the further application of questin as aquatic antibacterial agent.
KeywordsQuestin 2-Hydroxypropyl-β-cyclodextrin Inclusion complex Solubility Antibacterial activity
This work financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (CXKT20180216), Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX18_0942), Natural Science Foundation of Jiangsu Province (BK20151283), Technical Plan Project of Lianyungang (CG1612) and 521 Talented Project of Lianyungang (KKC17001).
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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