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3 Biotech

, 9:123 | Cite as

Characterization, solubility and antibacterial activity of inclusion complex of questin with hydroxypropyl-β-cyclodextrin

  • Lei GuoEmail author
  • Xi Cao
  • Shulin Yang
  • Xintong Wang
  • Ying Wen
  • Fei Zhang
  • Hui Chen
  • Le Wang
Short Reports
  • 32 Downloads

Abstract

To increase the water solubility of questin and broaden its application in preventing and treating Vibrio diseases in aquaculture, an inclusion complex of questin with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared by stirring and coevaporation strategy. The results of thin-layer chromatography and nuclear magnetic resonance spectrum confirmed the inclusion of questin into HP-β-CD. The aqueous solubility of questin in the inclusion complex reached (62.63 ± 1.21) µg/mL, which was 110 times of questin’s original solubility. The preliminary agar diffusion method indicated that questin–HP-β-CD inclusion complex showed enhanced antibacterial activity against Vibrio harveyi compared with free questin. This finding provided a reliable basis for the further application of questin as aquatic antibacterial agent.

Keywords

Questin 2-Hydroxypropyl-β-cyclodextrin Inclusion complex Solubility Antibacterial activity 

Notes

Acknowledgements

This work financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (CXKT20180216), Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX18_0942), Natural Science Foundation of Jiangsu Province (BK20151283), Technical Plan Project of Lianyungang (CG1612) and 521 Talented Project of Lianyungang (KKC17001).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Lei Guo
    • 1
    • 2
    • 3
    Email author
  • Xi Cao
    • 1
    • 2
  • Shulin Yang
    • 1
    • 2
  • Xintong Wang
    • 1
    • 2
  • Ying Wen
    • 2
  • Fei Zhang
    • 1
    • 2
  • Hui Chen
    • 1
    • 2
  • Le Wang
    • 1
    • 2
  1. 1.Jiangsu Key Laboratory of Marine Bioresources and EnvironmentHuaihai Institute of TechnologyLianyungangChina
  2. 2.Jiangsu Key Laboratory of Marine Biotechnology, Co-innovation Center of Jiangsu Marine Bio-industry TechnologyHuaihai Institute of TechnologyLianyungangChina
  3. 3.Jiangsu Institute of Marine Resources DevelopmentLianyungangChina

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