Advertisement

3 Biotech

, 9:126 | Cite as

Phosphate-solubilizing Bacillus sp. enhances growth, phosphorus uptake and oil yield of Mentha arvensis L

  • Jai Prakash
  • Naveen Kumar AroraEmail author
Original Article
  • 75 Downloads

Abstract

In the present study, phosphate solubilizing rhizobacterial isolate STJP from the rhizosphere of Stevia rebaudiana was identified as a Bacillus sp. on the basis of phenotypic, biochemical, and 16S rRNA gene sequencing. In addition to phosphate solubilization ability, isolate Bacillus sp. STJP produced a significant quantity of siderophore (16.06 µg/ml) and indole 3-acetic acid (30.59 µg/ml). In the greenhouse experiment, treatment with STJP along with tricalcium phosphate (TCP200) showed significant increase in the plant growth parameters, oil yield and P uptake in M. arvensis as compared to the control plants. Amongst all the treatments, highest oil yield and menthol content were observed when treated with Bacillus sp. STJP + TCP200. Hence, an integrated approach of using Bacillus sp. STJP along with TCP can be used to increase the production of menthol and oil yield of M. arvensis. This approach of using fertilizer along with phosphate solubilizing Bacillus sp. worked very well and was more effective in comparison with individual treatment of fertilizer or plant growth promoting rhizobacteria. A combined use of efficient phosphate solubilising bacteria loaded with plant growth promoting characters along with TCP can thus be far effective way for enhancing the yield of crops in a sustainable manner.

Keywords

Bacillus Mentha arvensis Menthol Plant growth promoting rhizobacteria Sustainable agriculture 

Notes

Acknowledgements

The authors are thankful to Vice Chancellor, Babasaheb Bhimrao Ambedkar University, Lucknow, India for his support.

Compliance with ethical standards

Conflict of interest

All authors declare that there is no conflict of interest in this original article.

References

  1. Abd_Allah EF, Alqarawi AA, Hashem A, Radhakrishnan R, Al-Huqail AA, Al-Otibi F, Malik JA, Alharbi RI, Egamberdieva D (2018) Endophytic bacterium Bacillus subtilis (BERA 71) improves salt tolerance in chickpea plants by regulating the plant defense mechanisms. J Plant Interact 13(1):37–44CrossRefGoogle Scholar
  2. Ahmad Z, Wu J, Chen L, Dong W (2017) Isolated Bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing PCR. Sci rep 7(1):1777CrossRefGoogle Scholar
  3. Ahmad M, Ahmad I, Hilger TH, Nadeem SM, Akhtar MF, Jamil M, Hussain A, Zahir ZA (2018) Preliminary study on phosphate solubilizing Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 for improving cotton growth under alkaline conditions. Peer J 6:e5122.  https://doi.org/10.7717/peerj.5122 CrossRefPubMedGoogle Scholar
  4. Alankar S (2009) A review on peppermint oil. Asian J Pharm Clin Res 2:4–6Google Scholar
  5. Alori ET, Glick BR, Babalola OO (2017) Microbial phosphorus solubilization and its potential for use in sustainable agriculture. Front Microbiol 8:971CrossRefGoogle Scholar
  6. Arora NK, Verma M (2017) Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria. 3 Biotech 7(6):381CrossRefGoogle Scholar
  7. Arora NK, Kang SC, Maheshwari DK (2001) Isolation of siderophore producing strain of Rhizobium meliloti and their biocontrol potential against Macrophomina phaseolina that causes charcoal rot of groundnut. Curr Sci 25:674–677Google Scholar
  8. Arora NK, Verma M, Prakash J, Mishra J (2016) Regulation of biopesticides: global concerns and policies. In: Bioformulations for sustainable agriculture. Springer, New Delhi, pp 283–299Google Scholar
  9. Bakry AM, Fang Z, Ni Y, Cheng H, Chen YQ, Liang L (2016) Stability of tuna oil and tuna oil/peppermint oil blend microencapsulated using whey protein isolate in combination with carboxymethyl cellulose or pollutant. Food Hydrocoll 60:559–571CrossRefGoogle Scholar
  10. Behera BC, Yadav H, Singh SK, Mishra RR, Sethi BK, Dutta SK, Thatoi HN (2017) Phosphate solubilization and acid phosphatase activity of Serratia sp. isolated from mangrove soil of Mahanadi river delta, Odisha, India. J Genet Eng Biotechnol 15:169–178CrossRefGoogle Scholar
  11. Brick JM, Bostock RM, Silverstone SE (1991) Rapid in situ assay for indoleacetic acid production by bacteria immobilized on nitrocellulose membrane. Appl Environ Microbiol 57:535–538Google Scholar
  12. Chakraborty U, Chakraborty BN, Chakraborty AP, Dey PL (2013) Water stress amelioration and plant growth promotion in wheat plants by osmotic stress tolerant bacteria. World J Microbiol Biotechnol 29(5):789–803CrossRefGoogle Scholar
  13. Chen M, Li Z, Huang P, Li X, Qu J, Wenyi Y, Qiwu Z (2017) Mechanochemical transformation of apatite to phosphoric slow-release fertilizer and soluble phosphate. Proc Saf Environ Prot 114:91–96CrossRefGoogle Scholar
  14. Garrity G (2005) The proteobacteria, Part B the gammaproteobacteria. In: Bergey’s manual of systematic bacteriology, vol 2. Springer, New York, pp 323–379CrossRefGoogle Scholar
  15. Golding CG, Lamboo LL, Beniac DR, Booth TF (2016) The scanning electron microscope in microbiology and diagnosis of infectious disease. Sci Rep 6:26–516CrossRefGoogle Scholar
  16. Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research. Wiley, New YorkGoogle Scholar
  17. Goswami D, Thakker JN, Dhandhukia PC (2016) Portraying mechanics of plant growth promoting rhizobacteria (PGPR): a review. Cogent Food Agric 2:1127500Google Scholar
  18. Gouda S, Kerry RG, Das G, Paramithiotis S, Shin HS, Patra JK (2018) Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture. Microbiol Res 206:131–140CrossRefGoogle Scholar
  19. Gupta M, Kiran S, Gulati A, Singh B, Tewari R (2012) Isolation and identification of phosphate solubilizing bacteria able to enhance the growth and aloin-A biosynthesis of Aloe barbadensis Miller. Microbiol Res 167:358–363CrossRefGoogle Scholar
  20. Hazzoumi Z, Moustakime Y, Joutei KA (2014) Effect of gibberellic acid (GA), indole acetic acid (IAA) and benzylaminopurine (BAP) on the synthesis of essential oils and the isomerization of methyl chavicol and trans-anethole in Ocimum gratissimum L. SpringerPlus 3(1):321CrossRefGoogle Scholar
  21. Khan AF, Mujeeb FARINA, Aha FAROOQI, Farooqui ALVINA (2015) Effect of plant growth regulators on growth and essential oil content in palmarosa (Cymbopogon martinii). Asian J Pharm Clin Res 8(2):373–376Google Scholar
  22. Koenig RA, Johnson CR (1942) Colorimetric determination of phosphorus in biological materials. Ind Eng Chem Anal 14:155–156CrossRefGoogle Scholar
  23. Koo SY, Cho KS (2009) Isolation and characterization of a plant growth-promoting rhizobacterium, Serratia sp. SY5. J Microbiol Biotechnol 19:1431–1438PubMedGoogle Scholar
  24. Kumar S, Suresh R, Singh V, Singh AK (2011) Economic analysis of menthol mint cultivation in Uttar Pradesh: a case study of Barabanki district. Agric Econ Res Review 24:345–350Google Scholar
  25. Langenau EE (1948) The examination and analysis of essential oils, synthesis and isolated. In: Guenther E (ed) The essential oils, vol 2. Van Nostrand, Princeton, pp 229–367Google Scholar
  26. Lateef A, Adelere IA, Gueguim-Kana EB (2015) The biology and potential biotechnological applications of Bacillus safensis. Biologia 4:411–419Google Scholar
  27. Line MA, Loutit MW (1971) Non-symbiotic nitrogen-fixing organisms from some New Zealand tussock-grassland soils. Microbiology 66(3):309–318Google Scholar
  28. Maheshwari DK, Dubey RC, Aeron A, Kumar B, Kumar S, Tewari S, Arora NK (2012) Integrated approach for disease management and growth enhancement of Sesamum indicum L. utilizing Azotobacter chroococcum TRA2 and chemical fertilizer. World J Microbiol Biotechnol 28(10):3015–3024CrossRefGoogle Scholar
  29. Maidak BL, Cole JR, Lilburn TG, Parker CT, Saxman PR, Stredwick JM, Garrity GM, Li B, Olsen GJ, Pramanik S, Schmidt TM, Tiedje JM (2000) The RDP (ribosomal database project) continues. Nucleic Acids Res 28:173–174CrossRefGoogle Scholar
  30. Malusà EM, Russo A, Mozzetti C, Belligno A (2006) Modification of secondary metabolism and flavonoid biosynthesis under phosphate deficiency in bean roots. J Plant Nutr 29:245–258CrossRefGoogle Scholar
  31. Mamta P, Rahi V, Pathania A, Gulati B, Singh RK, Bhanwra et al (2010) Stimulatory effect of phosphate-solubilizing bacteria on plant growth, stevioside and rebaudioside-A contents of Stevia rebaudiana Bertoni. Appl Soil Ecol 46:222–229CrossRefGoogle Scholar
  32. Millar RL, Higgins VJ (1970) Association of cyanide with infection of birdsfoot trefoil by Stemphylium loti. Phytopathol 60(1):104–110CrossRefGoogle Scholar
  33. Mishra J, Prakash J, Arora NK (2016) Role of beneficial soil microbes in sustainable agriculture and environmental management. Clim Change Environ Sustain 4:137–149CrossRefGoogle Scholar
  34. Mukhtar S, Shahid I, Mehnaz S, Malik KA (2017) Assessment of two carrier materials for phosphate solubilizing biofertilizers and their effect on growth of wheat (Triticum aestivum L.). Microbiol Res 205:107–117CrossRefGoogle Scholar
  35. Nautiyal CS (1999) An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett 170:265–270CrossRefGoogle Scholar
  36. Nell M, Vötsch M, Vierheilig H, Steinhellner S, Zitterl-Eglseer K, Franz C, Novak J (2009) Effect of phosphorus uptake on growth and secondary metabolites of garden sage (Salvia officinalis L). J Sci Food Agric 80:1090–1096CrossRefGoogle Scholar
  37. Parhamfar M, Badoei-Dalfard A, Parhamfar M, Fahimi Rad S (2016) Purification and characterization of an extracellular phosphatase enzyme from Bacillus spp. J Cell Mol Res 8(2):90–97Google Scholar
  38. Pereira SIA, Barbosa L, Castro PML (2015) Rhizobacteria isolated from a metal-polluted area enhance plant growth in zinc and cadmium-contaminated soil. Int J Environ Sci Technol 12:2127–2142CrossRefGoogle Scholar
  39. Perkin JE (1984) High-performance liquid chromatographic assay of menthol using indirect photometric detection. J Chromatogr 303:436–439Google Scholar
  40. Pikovskaya RI (1948) Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiologiya 17:362–370Google Scholar
  41. Radhakrishnan R, Hashem A, Abd Allah EF (2017) Bacillus: a biological tool for crop improvement through bio-molecular changes in adverse environments. Front Physiol 8:667CrossRefGoogle Scholar
  42. Reeves MW, Pine L, Neilands JB, Balows A (1983) Absence of siderophore activity in Legionella species grown in iron-deficient media. J Bacteriol 154:324–329PubMedPubMedCentralGoogle Scholar
  43. Ribeiro VP, Marriel IE, de Sousa SM, de Paula Lana UG, Mattos BB, de Oliveira CA, Gomes EA (2018) Endophytic Bacillus strains enhance pearl millet growth and nutrient uptake under low-P. Braz J Microbiol.  https://doi.org/10.1016/j.bjm.2018.06.005 CrossRefPubMedPubMedCentralGoogle Scholar
  44. Sharma SB, Sayyed RZ, Trivedi MH, Gobi TA (2013) Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus 2:587CrossRefGoogle Scholar
  45. Shen J, Yuan L, Zhang J, Li H, Bai Z, Chen X, Zhang W, Zhang F (2011) Phosphorus dynamics: from soil to plant. Plant Physiol 156:997–1005CrossRefGoogle Scholar
  46. Singh R, Arora NK (2016) Growth enhancement of medicinal plant Withania somnifera using phosphate solubilizing endophytic bacteria Pseudomonas sp. as bioinoculant. Inter J Sci Technol Soci 2:13–18Google Scholar
  47. Singh R, Soni SK, Patel RP, Kalra A (2013) Technology for improving essential oil yield of Ocimum basilicum L. (sweet basil) by application of bioinoculant colonized seeds under organic field conditions. Ind Crops Prod 45:335–342CrossRefGoogle Scholar
  48. Srivastava RK, Singh AK, Kalra A, Tomar VKS, Bansal RP, Patra DD, Chand S, Naqvi AA, Sharma S, Kumar S (2002) Characteristics of menthol mint Mentha arvensis cultivated on industrial scale in the Indo-Gangetic plains. Ind Crops Prod 15(3):189–198CrossRefGoogle Scholar
  49. Stutte GW (2016) Controlled environment production of medicinal and aromatic plants. Medicinal and aromatic crops: production, phytochemistry, and utilization. In: ACS symposium series, chapter 4, vol 1218, pp 49–63.  https://doi.org/10.1021/bk-2016-1218.ch004 (ISBN13: 9780841231276eISBN: 9780841231269$4) Google Scholar
  50. Swain MR, Laxminarayana K, Ray RC (2012) Phosphorus solubilization by thermotolerant Bacillus subtilis isolated from cow dung microflora. Agric Res 1(3):273–279CrossRefGoogle Scholar
  51. Tabatabai MA, Bremner JM (1969) Use of p-nitrophenol phosphate in assay of soil phosphatase activity. Soil Biol Biochem 1:301–307CrossRefGoogle Scholar
  52. Tewari S, Arora NK (2016) Fluorescent Pseudomonas sp. PF17 as an efficient plant growth regulator and biocontrol agent for sunflower crop under saline conditions. Symbiosis 68:99–108CrossRefGoogle Scholar
  53. Tewari S, Arora NK (2018) Role of salicylic acid from Pseudomonas aeruginosa PF23EPS + in growth promotion of sunfower in saline soils infested with phytopathogen Macrophomina phaseolina. Environ Sustain 1:49–59CrossRefGoogle Scholar
  54. Walpola BC, Yoon MH (2013) Isolation and characterization of phosphate solubilizing bacteria and their co-inoculation efficiency on tomato plant growth and phosphorous uptake. Afr J Microbiol Res 7:266–275Google Scholar
  55. Wei Y, Zhao Y, Shi M, Cao Z, Lu Q, Yang T, Fan Y, Wei Z (2018) Effect of organic acids production and bacterial community on the possible mechanism of phosphorus solubilization during composting with enriched phosphate-solubilizing bacteria inoculation. Bioresour Technol 247:190–199CrossRefGoogle Scholar
  56. World Health Organization (2013) WHO traditional medicine strategy 2014–2023. World Health Organization, GenevaGoogle Scholar
  57. Xu HX, Weng XY, Yang Y (2007) Effect of phosphorus deficiency on the photosynthetic characteristics of rice plants. Russ J Plant Physiol 54:741–748CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Environmental Microbiology (DEM), School for Environmental Sciences (SES)Babasaheb Bhimrao Ambedkar University (A Central University)LucknowIndia
  2. 2.Department of Environmental Science (DES), School for Environmental Sciences (SES)Babasaheb Bhimrao Ambedkar University (A Central University)LucknowIndia

Personalised recommendations