3 Biotech

, 9:121 | Cite as

Banana bunchy top virus (BBTV) nuclear shuttle protein interacts and re-distributes BBTV coat protein in Nicotiana benthamiana

  • Xiao-long Ji
  • Nai-tong YuEmail author
  • Ling Qu
  • Bin-bin Li
  • Zhi-xin LiuEmail author
Original Article


Banana bunchy top virus (BBTV) is a circular single-stranded DNA virus with multi-components. The knowledge about interaction between viral proteins and pathogenesis mechanism of BBTV remains unclear. In this study, the coat protein gene (CP, ORF 516 bp) and nuclear shuttle protein gene (NSP, ORF 465 bp) from BBTV B2 isolate of the Southeast-Asia group were cloned. The intracellular localization analysis showed the CP locates in the cell nucleus of tobacco cells, while the NSP distributes in the cell nucleus and cytoplasm. Co-localization analysis indicated the NSP itself does not change distribution, but CP re-distributes to the cell nucleus and cytoplasm, suggesting that NSP interacts with CP and re-locates the CP in the cell. The interaction between CP and NSP was further verified by co-immunoprecipitation (Co-IP) in tobacco protoplasts. The study will help us to understand the interaction between viral proteins and pathogenesis mechanism of BBTV in host plants.


Banana bunchy top virus CP NSP Intracellular localization Interaction Co-IP 



This work was supported by the National Natural Science Foundation of China (31401709), the Hainan Provincial Natural Science Foundation (20153130) and the Young Elite Scientists Sponsorship Program by CSTC (CSTC-QN201704).

Compliance with ethical standards

Conflict of interest

The authors have declared no conflict of interest.


  1. Bressan A, Watanabe S (2011) Immunofluorescence localisation of Banana bunchy top virus (family nanoviridae) within the aphid vector, pentalonia nigronervosa, suggests a virus tropism distinct from aphid-transmitted luteoviruses. Virus Res 155(2):520–525CrossRefGoogle Scholar
  2. Burns TM, Harding RM, Dale JL (1995) The genome organization of Banana bunchy top virus: analysis of six ssDNA components. J Gen Virol 76(6):1471–1482CrossRefGoogle Scholar
  3. Fontes EPB (2004) The geminivirus nuclear shuttle protein is a virulence factor that suppresses transmembrane receptor kinase activity. Genes Dev 18(20):2545–2556CrossRefGoogle Scholar
  4. Frischmuth S, Wege C, Hülser D et al (2007) The movement protein bc1 promotes redirection of the nuclear shuttle protein bv1 of abutilon mosaic geminivirus to the plasma membrane in fission yeast. Protoplasma 230(1–2):117–123CrossRefGoogle Scholar
  5. Ghag SB, Ganapathi TR (2018) Banana and Plantains: Improvement, Nutrition, and Health. In: Mérillon JM, Ramawat K (eds) Bioactive Molecules in Food. Reference Series in Phytochemistry. Springer, Cham, pp 1–20Google Scholar
  6. Gronenborn B (2004) Nanoviruses: genome organisation and protein function. Vet Microbiol 98(2):103–109CrossRefGoogle Scholar
  7. Krapp S, Greiner E, Amin B et al (2017) The stress granule component G3BP is a novel interaction partner for the nuclear shuttle proteins of the nanovirus Pea necrotic yellow dwarf virus and geminivirus abutilon mosaic virus. Virus Res 227:6–14CrossRefGoogle Scholar
  8. Kumar PL, Selvarajan R, Iskra-Caruana ML et al (2015) Chapter seven—biology, etiology, and control of virus diseases of banana and plantain. Adv Virus Res 91(1):229CrossRefGoogle Scholar
  9. Kumar P, Arun V, Lokeswari TS (2017) Cloning of BBTV (Banana bunchy top virus) components and screening of BBTV using functionalized gold nanoparticles. 3 Biotech 7(3):225CrossRefGoogle Scholar
  10. Li B, Yang Y, Luo Z et al (2018) Quantitative Screening of secretory protein genes in Candidatus Liberibacter Asiaticus. Am J Plant Sci 9(12):2408–2419CrossRefGoogle Scholar
  11. McGarry RC, Barron YD, Carvalho MF et al (2003) A novel arabidopsis acetyltransferase interacts with the geminivirus movement protein NSP. The Plant cell 15(7):1605CrossRefGoogle Scholar
  12. Niu S, Wang B, Guo X et al (2009) Identification of two RNA silencing suppressors from Banana bunchy top virus. Arch Virol 154(11):1775CrossRefGoogle Scholar
  13. Qazi J (2016) Banana bunchy top virus and the bunchy top disease. J Gen Plant Pathol 82(1):2–11CrossRefGoogle Scholar
  14. Sparkes IA, Runions J, Kearns A et al (2006) Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants. Nat Protocols 1(4) 2019CrossRefGoogle Scholar
  15. Stainton D, Martin DP, Collings DA et al (2016) Comparative analysis of common regions found in babuviruses and alphasatellite molecules. Arch Virol 162(3):1–7Google Scholar
  16. Wang X (2015) Protein-protein interactions between NSP and proteins of Banana bunchy top virus. Dissertation, Hainan UniversityGoogle Scholar
  17. Wanitchakorn R, Harding RM, Dale JL (1997) Banana bunchy top virus DNA-3 encodes the viral coat protein. Arch Virol 142(8):1673–1680CrossRefGoogle Scholar
  18. Wanitchakorn R, Hafner GJ, Harding RM et al (2000) Functional analysis of proteins encoded by Banana bunchy top virus DNA-4 to -6. J Gen Virol 81(Pt 1):299–306CrossRefGoogle Scholar
  19. Watanabe S, Bressan A (2013) Tropism, compartmentalization and retention of Banana bunchy top virus (Nanoviridae) in the aphid vector Pentalonia Nigronervosa. J Gen Virol 94(Pt1):691–697Google Scholar
  20. Wu F, Hanzawa Y (2018) A simple method for isolation of soybean protoplasts and application to transient gene expression analyses. J Visual Exp, 131,
  21. Yang JW, Fu JX, Li J et al (2014) A novel co-immunoprecipitation protocol based on protoplast transient gene expression for studying protein–protein interactions in rice. Plant Mol Biol Rep 32(1):153–161CrossRefGoogle Scholar
  22. Yu NT, Liu ZX (2011) New research advance of Banana bunchy top virus. Microbiol China 38(3):396–404Google Scholar
  23. Yu NT, Feng TC, Zhang YL et al (2011) Bioinformatic analysis of BBTV satellite DNA in Hainan. Virol Sinica 26(4):279–284CrossRefGoogle Scholar
  24. Yu NT, Zhang YL, Feng TC et al (2012) Cloning and sequence analysis of two Banana bunchy top virus genomes in Hainan. Virus Genes 44(3):488–494CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Microbiology, Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikouChina

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