Phylogenetic diversity of sulfate-reducing bacteria of sediments of Chilika Lake, India, determined through analysis of the dissimilatory sulfite reductase (dsr AB) gene
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In this study, the sulfate-reducing bacteria, (SRB) were identified and reported for the first time through analysis of functional gene dsrAB, from the DNA of sediment samples collected from 10 sites of the Chilika lake. The finding illustrates Forty six Operational Taxonomic Units (OTUs), identified from the DGGE which were obtained from the 10 sediment samples. Of these, 34 OTUs exhibited around 78–96% sequence similarity and 12 OTUs showed 97 to 100% sequence similarity to the dsrAB gene of reported type strains of SRB. The sequence information obtained revealed the presence and distribution of diverse types of SRB which include phylotypes related to Desulfovibrio, Desulfonatronovibrio, Desulfomicrobium, Desulfobotulous and Desulfobacca. Upon comparison of dsrAB gene sequences of SRB obtained through this study with those collected from the GenBank, and through the dendrogram constructed, it was observed that except 13 OTUs that clustered closely with the reported type strains, all other 36 OTUs clustered distantly and had no representative member of SRB. This indicated the presence of phylogenetically diverse groups of SRB inhabiting the lake Chilika.
KeywordsSulfate-reducing bacteria Sediments Desulfovibrio Dissimilatory sulfite reductase DsrAB gene Chilika Lagoon Operational taxonomic units (OTU’s)
TS Sasi Jyothsna thanks CSIR for the award of research fellowship. Financial assistance received from DBT and DST (FIST) is acknowledged. We thank Ms. Azmatunnisa for the technical support. Also, I would like to extend my gratitude to Dr. B. Chakradhar, Director, RESPL, for critically reviewing the manuscript and serving as scientific advisor.
Compliance with ethical standards
Conflict of interest
The authors state no conflict of interest for this work.
- Barton LL (1995) Sulfate reducing bacteria. In: Atkinson T, Sherwood RF Biotechnology handbooks-8. Series Kluwer Academic/ Plenum Publishers, New YorkGoogle Scholar
- Bidle KA, Kastner M, Bartlett DH (1999) A phylogenetic analysis of microbial communities associated with the methane hydrate containing marine fluids and sediments in the Cascadia Margin (ODP site 892B). FEMS Microbiol Lett 177:101–108. https://doi.org/10.1111/j.1574-6968.1999.tb13719.x CrossRefPubMedGoogle Scholar
- Eaton AD, Clesceri LS, Greenberg AE, Franson MAH (2005) Standard methods for the examination of water and wastewater. Am Public Health Assoc 21:1600Google Scholar
- Edgcomb VP, McDonald JH, Devereux R, Smith DW (1999) Estimation of bacterial cell numbers in humic-rich salt marsh sediments using probes to 16S rDNA. Appl Environ Microbiol 164:271–279Google Scholar
- Fowler CMR (1990) The solid earth, an introduction to global geophysics. Cambridge University Press, CambridgeGoogle Scholar
- Jørgensen BB (1983) Processes at the sediment water interface. In: Bolin B, Cook RB (eds) The major biochemical cycles and their interactions. Wiley, Chichester, pp 447–515Google Scholar
- Karr EA, Sattley WM, Rice MR, Jung DO, Madigan MT, Achenbach LA (2005) Diversity and distribution of sulfate-reducing bacteria in permanently frozen LakeFryxell McMurdo Dry Valleys Antarctica. Appl Environ Microbiol 71:6353–6359. https://doi.org/10.1128/AEM.71.10.6353-6359.2005 CrossRefPubMedPubMedCentralGoogle Scholar
- Köpke B, Wilms R, Engelen B, Cypionka H, Sass H (2005) Microbial diversity in coastal subsurface sediments: a cultivation approach using various electron acceptors and substrate gradients. Appl Environ Microbiol 71:7819–7830. https://doi.org/10.1128/AEM.71.12.7819-7830.2005 CrossRefPubMedPubMedCentralGoogle Scholar
- Mohanty PK, Panda B (2009) Circulation & mixing processes in Chilika Lagoon. Indian J Mar Sci 38:205–214. http://nopr.niscair.res.in/handle/123456789/4671
- Throckmorton HM, Heikoop JM, Newman BD, Altmann GL, Conrad MS, Muss JD, Wilson CJ (2015) Pathways and transformations of dissolved methane and dissolved inorganic carbon in Arctic tundra watersheds: Evidence from analysis of stable isotopes. Glob Biogeochem Cycles 29:1893–1910. https://doi.org/10.1002/2014GB005044 CrossRefGoogle Scholar
- Wanger M, Roger AJ, Flax JL, Brusseau GA, Stahl DA (1998) Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration. J Bacteriol 180:2975–2982Google Scholar