Screening of high-virulent entomopathogenic fungal strains to infect Xylotrechus rusticus larvae
The gray tiger longicorn beetle, Xylotrechus rusticus Linnaeus (Coleoptera: Cerambycidae) is a stem-boring pest that can inhibit not only the transportation of nutrients in the trunk but also the tree growth, increasing the risk of tree breakage and causing economic losses. It is distributed in China, Iran, Turkey, Russia, Korea, Japan, and Southern Europe. This study aimed to investigate selected Beauveria strains that could be used as entomopathogenic fungi for the biological control of this pest. The high-virulence strains were screened among the selected strains by cumulative mortality, correct mortality, and lethal time to 50% mortality (LT50). These screened high-virulence strains were Bb01, CFCC83486, and CFCC81428. Bb01 exhibited 96.96% cumulative mortality, with an estimated LT50 of 3.28 days. CFCC83486 and CFCC81428 caused 89.29% and 75.74% cumulative mortality, with an estimated LT50 of 3.45 and 4.28 days, respectively. Pathogenicity at different concentrations and lethal concentration of 50% (LC50) of these high-virulence strains were investigated. The pathogenicity was found to be positively correlated with suspension concentration, and LC50 was negatively correlated with infection time. These suspensions of high-virulence strains at different concentrations were also investigated in the forest by brushing the suspensions on the poplar tree trunk infested with X. rusticus L. larvae. The most effective strain was found to be Bb01, whose cumulative mortality reached 76.33% at 1.32 × 108 conidia mL−1, followed by the strain CFCC83486, whose cumulative mortality reached 65.17% at 1.32 × 108 conidia mL−1. This study provides an important basis for using B. bassiana in the biological control of X. rusticus L.
KeywordsBeauveria bassiana Beauveria brongniartii Pathogenicity Fungal Gray tiger longicorn beetle Biocontrol
This article was supported by the National Natural Science Foundation of China (NSFC) [Grant number: 31370649].
Compliance with ethical standards
Conflict of interest
No potential conflict of interest was reported by the authors.
- Cao QJ, Chi DF (2017) Screening of high virulent entomopathogenic fungal strains to infect larvae of Cryptorrhynchus lapathi (Coleoptera: Curculionidae). Sci Silvae Sin 53(1):119–127Google Scholar
- Castrillo LA, Griggs MH, Vandenberg JD (2016) Competition between biological control fungi and fungal symbionts of ambrosia beetles Xylosandrus crassiusculus and X. germanus (Coleoptera: Curculionidae): mycelial interactions and impact on beetle brood production. Biol Control 103:138–146CrossRefGoogle Scholar
- Cherry AJ, Abalo K, Hell K, Korie S (2007) Farm-scale trials to compare the entomopathogenic fungus Beauveria bassiana with pirimiphos methyl + deltamethrin and essential oil of lemon grass for protection of stored cowpea against Callosobruchus maculatus (Coleoptera: Bruchidae). Ann Appl Biol 151(1):1–10CrossRefGoogle Scholar
- Ding JN, Yu J, Chi DF (2016) Study on the biocontrol of Xylotrechus rusticus L. by parasitic enemies. J Nanjing For Univ (Nat Sci Edn) 40(4):107–112Google Scholar
- Gu LQ, Li CX, Zhang SH (2009) Indoor toxicity test of Beauveria bassiana and Beauveria brongniartii to Spodoptera exigua Hiibner. Jiangsu Agric Sci 41(3):118–120Google Scholar
- He YR, Lu LH, Kuang ZB, Feng X, Chen HY, Wu YJ (2005) Effect of temperature and humidity on the virulence of beetle-derived Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes: Moniliales) against the daikon leaf beetle, Phaedon brassicae Baly (Coleoptera: Chrysomelidae). Acta Entomol Sin 48(5):679–686Google Scholar
- Hu CX, Huang YH, Li CJ, Sun JH (2004) Larval spatial distribution pattern of Xylotechus rusticus. Entomol Knowl 41(3):241–244Google Scholar
- Hu JF, Miao K, Dong ZH, Han B, Fan MZ, Li ZZ (2006) Field trial of application of Beauveria bassiana against Monochamus alternatus. J Anhui Agric Univ 33(3):332–336Google Scholar
- Li HP, Huang DZ, Wang ZG, Yan HX, Zheng JW (2007) Screening test of highly virulent strains of entomopathogenic fungi Beauveria bassiana against Apriona germari. Larvae Sci Silvae Sin 43(11):66–71Google Scholar
- Qian JJ, Chi DF, Ding JN (2015) Biological characteristics of Beauveria bassiana Bb01 strain. J Anhui Agric Sci 43(27):374–379Google Scholar
- Su P, Liao XL, Zhang Y, Xue ZJ (2009) Advance in controlling Holotrichia diomphalia by Beauveria bassiana in peanut field. Guangxi Agric Sci 40(4):373–377Google Scholar
- Wang ZM, Liu GR (2005) Distribution damage and assessment of forestry quarantine pests in Jilin province. For Pest Dis 24(5):19–21Google Scholar
- Wang YL, Yang XS, Tan LJ, Wang LJ (2002) Chemical control methods of Xylotechus rusticus L. For Sci Technol 27(3):26Google Scholar
- Wang GC, Zhang WD, Song TT, Chen RY (2003) Huangpu port intercepted Cyrtoclytus capra (Germar) and Xylotrechus rusticus L. Plant Quar 17(2):90Google Scholar
- Wen P, Wang H (2010) Harm and control technology of Xylotechus rusticus L.. Min Ying Ke Ji 1:92Google Scholar
- Zhao GH (2011) A study on the real value of poplar. Pract For Technol 8:20–22Google Scholar