Engineering of phosphoenolpyruvate: carbohydrate phosphotransferase system increased acetate assimilation in Escherichia coli
Acetate is a potential low-cost carbon source and can be used for microbial production of valuable chemicals in Escherichia coli. In this study, separate and simultaneous inactivation of the ptsG, ptsI, and ptsP genes involved in the phosphoenolpyruvate:carbohydrate phosphotransferase system was performed in E. coli and the effects on cell growth and acetate assimilation were evaluated. The mutant strain with double deletion of ptsG and ptsP exhibited faster acetate use than the other mutants. Inactivation of ptsI seriously reduced acetate consumption. This work provides a novel engineering target for improving acetate use rates.
KeywordsAcetate Phosphoenolpyruvate:carbohydrate phosphotransferase system Metabolic engineering Escherichia coli
This work was financially supported by the National Natural Science Foundation of China (31600066, 31100088, 31870105), the Shandong Provincial Natural Science Foundation (ZR2016CB20, ZR2016CL02), and the State Key Laboratory of Microbial Technology Open Projects Fund (M2016-10).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
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