3 Biotech

, 9:65 | Cite as

Association of a ‘Candidatus Phytoplasma aurantifolia’-related strain with apricot showing European stone fruit yellows symptoms in Iran

  • Rasoul Rasoulpour
  • Mohammad SalehiEmail author
  • Assunta Bertaccini
Original Article


During 2012–2015 surveys in some orchards in Faraghe (Iran), a number of apricot trees showed symptoms resembling those associated with the phytoplasma disease known as European stone fruit yellows that are severe leaf roll, yellowing and die back. The presence of an infectious agent was confirmed by graft transmission experiments in which all the previously symptomless GF-677 (peach × almond) trees showed phytoplasma-type symptoms. The phytoplasma presence was confirmed by nested PCR assays using DNA extracted from samples from both field collected and graft-inoculated trees. The sequences of four nested PCR products from symptomatic apricot and experimentally graft-inoculated GF-677 trees were 100% identical to each other. RFLP and phylogenetic analyses carried out on these sequences allowed to cluster the strain infecting apricot trees in Iran with the16SrII-C subgroup phytoplasmas. This is the first report of a 16SrII-C phytoplasma associated with leaf roll and yellowing of the leaves in apricot trees.


Stone fruits Apricot tree Leaf roll Yellowing 16SrII phytoplasma 



This research was supported by a Grant from Fars Agricultural and Natural Resources Research and Education Center, AREEO, Zarghan, Iran.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest.


  1. Allahverdi T, Rahimian H, Babaeizad V (2014) Association of ‘Candidatus Phytoplasma prunorum’ with plum yellow leaf stunt in Iran. Iran J Plant Pathol 50:99Google Scholar
  2. Bertaccini A, Lee I-M (2018) Phytoplasmas: un update. In: Rao GP, Bertaccini A, Fiore N, Liefting L (eds) Phytoplasmas: plant pathogenic bacteria—I. Springer Nature Singapore Pte Ltd., Singapore, pp 1–30Google Scholar
  3. Bertaccini A, Duduk B, Paltrinieri S, Contaldo N (2014) Phytoplasmas and phytoplasma diseases: a severe threat to agriculture. Am J Plant Sci 5:1763–1788CrossRefGoogle Scholar
  4. Cieslinska M (2011) European stone fruit yellows disease and its causal agent ‘Candidatus phytoplasma prunorum’. J Plant Prot Res 5:441–447Google Scholar
  5. Cvrković A, Jović J, Mitrović M, Krstić O, Krnjalić S, Toševski I (2011) Potential new hemipteran vectors of “stolbur” phytoplasma in Serbian vineyards. Bull Insect 64(Suppl):S129–S130Google Scholar
  6. Deng S, Hiruki C (1991) Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. J Microbiol Methods 14:53–61CrossRefGoogle Scholar
  7. Esmailzadeh Hosseini SA, Khodakaramian G, Salehi M, Fani SR, BolokYazdi HR, Raoufi D, Jadidi O, Bertaccini A (2015) Status of alfalfa witches’ broom phytoplasma disease in Iran. Phytopathog Mollicutes 5(1-Suppl):S65–S66CrossRefGoogle Scholar
  8. Faghihi MM, Siampour M, Zaeifi M, Bagheri AN, Salehi M, Samavi S (2010) First report of a phytoplasma associated with Periploca aphylla witches’ broom in Iran. Plant Pathol 59:400CrossRefGoogle Scholar
  9. Foissac X, Wilson MR (2010) Current and possible future distributions of phytoplasma diseases and their vectors. In: Weintraub PG, Jones P (eds) Phytoplasmas: genomes plant hosts and vectors. CAB International, OxfordGoogle Scholar
  10. Ghayeb Zamharir M, Paltrinieri S, Hajivand S, Taheri M, Bertaccini A (2017) Molecular identification of diverse ‘Candidatus Phytoplasma’ species associated with grapevine decline in Iran. J Phytopathol 165:407–413CrossRefGoogle Scholar
  11. Gundersen DE, Lee I-M (1996) Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Medit 35:144–151Google Scholar
  12. Jarausch W, Jarausch-Wertheim B, Danet J-L, Broquaire JM, Dosba F, Saillard C, Garnier M (2001) Detection and identification of European stone fruit yellows and other phytoplasmas in wild plants in the surroundings of apricot chlorotic leaf roll-affected orchards in southern France. Eur J Plant Pathol 107:209–217CrossRefGoogle Scholar
  13. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  14. Lee I-M, Gundersen-Rindal DE, Davis RE, Bartoszyk IM (1998) Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 48:1153–1169CrossRefGoogle Scholar
  15. Lee I-M, Davis RE, Gundersen-Rindal DE (2000) Phytoplasma: phytopathogenic mollicutes. Annu Rev Microbiol 54:221–255PubMedCrossRefGoogle Scholar
  16. Necas T, Krska B (2005) Detection of phytoplasma ESFY in apricot trees using phloem and petioles. Plant Prot Sci 41:132–140CrossRefGoogle Scholar
  17. Orságová H, Březíková M, Schlesingerová G (2011) Presence of phytoplasmas in hemipterans in Czech vineyards. Bull Insect 64(Suppl):S119–S120Google Scholar
  18. Rasoulpour R, Salehi M, Salehi E (2017) Detection and partial characterization of a 16SrIX-C phytoplasma associated with hemp (Cannabis sativa L.) witches’ broom in Iran. J Plant Pathol 99:219–223Google Scholar
  19. Salehi M, Esmailzadeh Hosseini SA (2017) First report of a 16SrXI group phytoplasma (‘Candidatus phytoplasma oryzae’) associated with Cyperus spp. white leaf disease in Iran. J Plant Pathol 99:809Google Scholar
  20. Salehi M, Heydarnejad J, Izadpanah K (2005) Molecular characterization and grouping of 35 phytoplasmas from central and southern provinces of Iran. Iran J Plant Pathol 41:62–64Google Scholar
  21. Salehi M, Izadpanah K, Heydarnejad J (2006) Characterization of a new almond witches’ broom phytoplasma in Iran. J Phytopathol 154:386–391CrossRefGoogle Scholar
  22. Salehi M, Siampour M, Esmailzadeh Hosseini SA, Bertaccini A (2015) Characterization and vector identification of phytoplasmas associated with cucumber and squash phyllody in Iran. Bull Insectol 68:311–319Google Scholar
  23. Salehi M, Esmailzadeh Hosseini SA, Salehi E, Bertaccini A (2016) Molecular and biological characterization of a 16SrII phytoplasma associated with carrot witches’ broom in Iran. J Plant Pathol 98:83–90Google Scholar
  24. Schneider B, Seemüller E, Smart CD, Kirkpatrick BC (1995) Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In: Razin S, Tully JC (eds) Molecular and diagnostic procedures in mycoplasmology, vol 1. Academic, San Diego, pp 369–380CrossRefGoogle Scholar
  25. Zhang YP, Uyemoto JK, Kirkpatrick BC (1998) A small-scale procedure for extracting nucleic acids from woody plants infected with various phytoplasmas for PCR assay. J Virol Methods 71:45–50PubMedCrossRefGoogle Scholar
  26. Zhao Y, Davis RE (2016) Criteria for phytoplasma 16Sr group/subgroup delineation and the need of a platform for proper registration of new groups and subgroups. Int J Syst Evol Microbiol 66:2121–2123PubMedCrossRefGoogle Scholar
  27. Zhao Y, Wei W, Lee I-M, Shao J, Suo X, Davis RE (2009) Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int J Syst Evol Microbiol 59:2582–2593PubMedPubMedCentralCrossRefGoogle Scholar
  28. Zirak L, Bahar M, Ahoonmanesh A (2009a) Characterization of phytoplasmas associated with almond diseases in Iran. J Phytopathol 157:736–741CrossRefGoogle Scholar
  29. Zirak L, Bahar M, Ahoonmanesh A (2009b) Molecular characterization of phytoplasmas related to peanut witches’ broom and “stolbur” groups infecting plum in Iran. J Plant Pathol 91:713–716Google Scholar
  30. Zirak L, Bahar M, Ahoonmanesh A (2010) Characterization of phytoplasmas related to ‘Candidatus Phytoplasma asteris’ and peanut WB group associated with sweet cherry diseases in Iran. J Phytopathol 158:63–65CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Rasoul Rasoulpour
    • 1
  • Mohammad Salehi
    • 1
    Email author
  • Assunta Bertaccini
    • 2
  1. 1.Plant Protection Research DepartmentFars Agricultural and Natural Resources Research and Education Center, AREEOZarghanIran
  2. 2.Department of Agricultural and Food SciencesAlma Mater Studiorum-University of BolognaBolognaItaly

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