3 Biotech

, 9:67 | Cite as

Low-cost shoot multiplication and improved growth in different cultivars of Canna indica

  • Dharmendra Kumar Purshottam
  • Ramesh Kumar Srivastava
  • Pratibha MisraEmail author
Original Article


Canna (Canna indica L.) is an ornamental landscape plant used specially for the garden borders and beds. It grows in tropical and subtropical countries including India. Canna is a less explored crop, mainly because it is a slow growing monocot with extremely hard seed coat and difficult to establish in vitro, as bacterial contamination is carried through the soil-grown rhizome. Many cultivars (ca. 150) of canna are being maintained in the garden germplasm of National Botanical Research Institute. To obtain 100% in vitro seed germination, chipping off of seeds with a sterilized nail clipper and soaking for 24–48 h or until radical emergence was a prerequisite. To obtain a foolproof tissue culture protocol of canna, in the present study, shoot multiplication was obtained through rhizome axillary buds. Among semisolid, liquid submerged and liquid media with glass beads, the highest multiplication of shoots (10) was obtained in liquid media with glass beads in ‘Canna Flaccida’ cv. within 6 weeks of culture incubation. During a comparative analysis of shoot regeneration among ten most attractive selected cultivars of canna, two did not respond, whereas a significant difference was obtained among eight cultivars. The regenerated shoots were rooted, acclimatized, and transferred to the pots, where they grew normally.


Cultivars (cvs.) Glass beads Rhizome axillary buds In vitro-seed germination Shoot regeneration Canna indica L. 



The authors are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow, for the facilities provided. This study was funded by Council of Scientific and Industrial Research-NBRI, through the Project no. OLP-0088.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest with the authors.


  1. Ahirwar MK, Mondal S, Singh MK, Sen C, Singh RP (2012) A high frequency plantlets regeneration protocol for banana (Musa paradisiaca L.) micropropagation. Asian J Hortic 7:397–401Google Scholar
  2. Avirutnant W, Pongpan A (1983) The antimicrobial activity of some Thai flowers and plants. Mahidol Univ J Pharm Sci 10:81–86Google Scholar
  3. Duke JA, Ayensu ES (1985) Medicinal plants of China, vol 1–2. Reference Publications, AlgonacGoogle Scholar
  4. Goel MK, Kukreja AK, Khanuja SPS (2007) Cost-effective approaches for in vitro mass propagation of Rauvolfia serpentina Benth. Ex Kurz. Asian J Plant Sci 6:957–961CrossRefGoogle Scholar
  5. Hosoki T, Sasaki H (1991) In vitro propagation of Canna edulis Ker. by longitudinal shoot split method. Plant Tiss Cult Lett 8:175–178CrossRefGoogle Scholar
  6. Imani AF, Sardoei AS, Shahdadneghad M (2014) Effect of H2SO4 on seed germination and viability of Canna indica L. ornamental plant. Intl J Adv Biol Biomed Res 2:223–229Google Scholar
  7. Joshi SC, Pant SC (2010) Effect of H2SO4 on seed germination and viability of Canna indica L. medicinal plant. J Am Sci 6:24–25Google Scholar
  8. Kromer K (1979) Biological activity of endogenous and influence of exogenous growth regulators on Canna indica regeneration in vitro. Acta Hortic 91:295–300CrossRefGoogle Scholar
  9. Kromer K, Kukulczanka K (1984) In vitro cultures of meristem tips of Canna indica. In: Rudnicki RM (ed) II symposium on growth regulators in floriculture, vol 167, pp 279–286Google Scholar
  10. Kromer K, Kukulczanka K (1985) In vitro cultures of meristem tips of Canna indica. Acta Hortic 167:279–286CrossRefGoogle Scholar
  11. Kumari A, Kumar S, Raj SK (2014) First report of Canna yellow mottle virus. on Canna from India. New Dis Rep 29:9CrossRefGoogle Scholar
  12. Mishra T, Goyal AK, Sen A (2015) An overview on the in vitro regeneration of Canna. Int J Fund Appl Sci 4:39–49CrossRefGoogle Scholar
  13. Murashige T, Skoog FA (1962) Revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473–497CrossRefGoogle Scholar
  14. Pandey VP, Ghosh S, Cherian E, Patani A (2013) Glass beads in liquid media: an alternative matrix for in vitro root induction of Cephaelis ipecacuanha A. Richard. Indian J Plant Physiol 18:388–391. CrossRefGoogle Scholar
  15. Pornsiriprasert D, Picha P, Preechanukool K, Ketsa-ard K, Temcharoen P, Chalermsanyakorn P, Chulsuri MU (1986) Studies on the antitumor activity of a Thai folkloric remedy: traditional medicinal plants. Asian J Pharm 6:124Google Scholar
  16. Roy SO, Bantawa P, Ghosh SK, Teixeira de Silva JA, Ghosh PD, Mondal TK (2010) Micropropagation and field performance of malbhog (Musa paradisiaca, AAB group): a popular banana cultivar with high keeping quality of North East India. Tree For Sci Biotechnol 4:52–58Google Scholar
  17. Sakai T, Imai K (2007) The influences of growth regulators and culture medium composition on shoot-tip cultures of edible Canna. Environ Control Biol 45:155–163CrossRefGoogle Scholar
  18. Sharma SC, Goel AK (1994) The National Botanical Research Institute (NBRI) Botanic Garden—a National Facility for India. BGCI, 2, May 1994Google Scholar
  19. Tanaka N (2004) The utilization of edible Canna plants in southeastern Asia and southern China. Econ Bot 58:112–114CrossRefGoogle Scholar
  20. Thepouyporn A, Yoosook C, Chuakul W, Thirapanmethee K, Napaswad C, Wiwat C (2012) Purification and characterization of anti-HIV-1 protein from Canna indica L. leaves. Southeast Asian J Trop Med Public Health 43:1153–1159PubMedGoogle Scholar
  21. Wafa SN, Taha RM, Mohajer S, Mahmad N, Abdul BAA (2016) Organogenesis and ultrastructural features of in vitro grown Canna indica L. BioMed Res Int 1–9.
  22. Woradulayapinij W, Soonthornchareonnon N, Wiwat C (2005) In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes. J Ethnopharm 101:84–89CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Plant BiotechnologyCSIR-National Botanical Research Institute (NBRI)LucknowIndia
  2. 2.CSIR-Central Institute of Medicinal and Aromatic PlantsLucknowIndia

Personalised recommendations