Overexpressing wheat low-molecular-weight glutenin subunits in rice (Oryza sativa L. japonica cv. Koami) seeds
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Genes encoding wheat low-molecular-weight glutenin subunits (LMW-GSs) that confer dough strength and extensibility were previously identified from Korean wheat cultivars. To improve low viscoelasticity of rice (Oryza sativa L.) dough caused by the lack of seed storage proteins comparable to wheat gluten, two genes, LMW03 and LMW28, encoding LMW-GSs are cloned from Korean wheat cultivar Jokyoung. The LMW genes are inserted into binary vectors under the control of the rice endosperm-specific Glu-B1 promoter. Transgenic rice plants expressing LMW03 or LMW28 in their seeds are generated using Agrobacterium-mediated transformation. The expression of recombinant wheat LMW-GS in the transgenic rice seeds was confirmed by SDS-PAGE and immunoblot analysis. Their accumulation in the endosperm and aleurone layers of rice seeds was observed through in situ immuno-hybridization.
KeywordsLow-molecular-weight glutenin subunit Transgenic rice Wheat Seed storage protein
This work was supported by grants from the National Institute of Agricultural Science (RDA PJ012458) and the Next-Generation BioGreen 21 Program (RDA PJ013149 and RDA PJ013159), South Korea. This study was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1A6A3A11931182) to KC.
YMJ designed and conducted the experiments; KC conducted the experiments and wrote the manuscript; SHL, JYK, and OH contributed critical reading and revision of the manuscript; JYL contributed scientific advice and corrected the manuscript. All authors have read and approved the manuscript.
Compliance with ethical standards
Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
- Altpeter F, Popelka JC, Wieser H (2004) Stable expression of 1Dx5 and 1Dy10 high-molecular-weight glutenin subunit genes in transgenic rye drastically increases the polymeric glutelin fraction in rye flour. Plant Mol Biol 54(6):783–792. https://doi.org/10.1007/s11103-004-0122-5 PubMedCrossRefGoogle Scholar
- Cameron-Mills V, Brandt A (1988) A gamma-hordein gene. Plant Mol Biol (4):449–461. https://doi.org/10.1007/BF00039026
- Gadaleta A, Blechl AE, Nguyen S, Cardone MF, Ventura M, Quick JS, Blanco A (2008) Stably expressed d-genome-derived HMW glutenin subunit genes transformed into different durum wheat genotypes change dough mixing properties. Mol Breed 22(2):267–279. https://doi.org/10.1007/s11032-008-9172-8 CrossRefGoogle Scholar
- Gao S, Gu YQ, Wu J, Coleman-Derr D, Huo N, Crossman C, Jia J, Zuo Q, Ren Z, Anderson OD, Kong X (2007) Rapid evolution and complex structural organization in genomic regions harboring multiple prolamin genes in the polyploid wheat genome. Plant Mol Biol 65(1–2):189–203. https://doi.org/10.1007/s11103-007-9208-1 PubMedCrossRefGoogle Scholar
- Lee J-Y, Kim Y-T, Kim B-M, Lee J-H, Lim S-H, Ha S-H, Ahn S-N, Nam M-H, Kim Y-M (2010) Cloning of low-molecular-weight glutenin subunit genes and identification of their protein products in common wheat (Triticum aestivum L.). Korean J Breed Sci 42(5):547–554Google Scholar
- Lee JY, Beom HR, Altenbach SB, Lim SH, Kim YT, Kang CS, Yoon UH, Gupta R, Kim ST, Ahn SN, Kim YM (2016) Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety. Funct Integr Genom 16(3):269–279. https://doi.org/10.1007/s10142-016-0482-3 CrossRefGoogle Scholar
- Shin M (2009) Rice-processed food. Food Sci Ind 42:2–18Google Scholar
- Shyur L-F, Wen T-N, Chen C-S (1994) Purification and characterization of rice prolamins. Bot Bull Acad Sin 35:65–71Google Scholar
- Yamagata H, Tanaka K (1986) The site of synthesis and accumulation of rice storage proteins. Plant Cell Physiol 27(1):135–145Google Scholar
- Zhang XF, Liu DC, Zhang JH, Jiang W, Luo GB, Yang WL, Sun JZ, Tong YP, Cui DQ, Zhang AM (2013) Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat. J Exp Bot 64(7):2027–2040. https://doi.org/10.1093/jxb/ert070 PubMedPubMedCentralCrossRefGoogle Scholar