In vitro fermentation of arabinoxylan from oat (Avena sativa L.) by Pekin duck intestinal microbiota
Arabinoxylan (AX) is abundant in cereal grains used as feed for ducks. However, the duck intestinal microbes responsible for the degradation of AX are not fully understood. In this study, oat AX was degraded and utilized by different duck intestinal microbiota in vitro. Changes in short-chain fatty acids (SCFAs), branch-chain fatty acids, and the pH resulted from a 72-h AX fermentation in intestinal samples were measured. The addition of AX increased the concentration of isobutyric acid and decreased the concentrations of SCFAs. The pH values decreased significantly in the intestinal samples. Gut microbiota were assessed using high-throughput sequencing of the 16S ribosomal RNA gene, and the results indicated that AX stimulated the growth of Megamonas and Bifidobacterium species, with Megamonas exhibiting the greatest stimulation. Overall, the results suggest that oat AX is utilized by specific bacteria in duck intestines, providing the theoretical basis for the impacts of AX on animal health.
KeywordsArabinoxylan Microbiome Hydrolysis 16S rRNA Fermentation
This work was supported by the Beijing Municipal Science and Technology Project (Grant number D161100006116002), and the National Natural Science Foundation of China (Grant number 31572440).
Compliance with ethical standards
Conflict of interest
All the authors declare that there are no conflicts of interest.
- Amato KR, Yeoman CJ, Kent A, Righini N, Carbonero F, Gaskins A, Gaskins HR, Stumpf RM, Yildirim S, Torralba M, Gillis M, Wilson BA, Nelson KE, White BA, Leigh SR (2013) Habitat degradation impacts black howler monkey (Alouatta pigra) Gastrointestinal Microbiomes. ISME J 7:1344–1353CrossRefGoogle Scholar
- Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc B 57:289–300Google Scholar
- Choi KY, Lee TK, Sul WJ (2015) Metagenomic analysis of chicken gut microbiota for improving metabolism and health of chickens—a review, Asian-Australas. J Anim Sci 28:1217–1225Google Scholar
- Cleemput G, Roels SP, Vanoort MP, Grobet J, Delcour JA (1993) Heterogeneity in the structure of water-soluble arabinoxylans in European wheat flours of variable bread-making quality. Cereal Chem 70:324–329Google Scholar
- Despres J, Forano E, Lepercq P, Comtet-Marre S, Jubelin G, Chambon C, Yeoman CJ, Berg Miller ME, Fields CJ, Martens E, Terrapon N, Henrissat B, White BA, Mosoni P (2016) Xylan degradation by the human gut Bacteroides xylanisolvens XB1A(T) involves two distinct gene clusters that are linked at the transcriptional level. BMC Genom 17:326CrossRefGoogle Scholar
- Grootaert C, Van den Abbeele P, Marzorati M, Broekaert WF, Courtin CM, Delcour JA, Verstraete W, Van de Wiele T (2009) Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem. FEMS Microbiol Ecol 69:231–242CrossRefGoogle Scholar
- Kim DY, Shin DH, Jung S, Kim H, Lee JS, Cho HY, Bae KS, Sung CK, Rhee YH, Son KH, Park HY (2014) Novel alkali-tolerant GH10 endo-β-1,4-xylanase with broad substrate specificity from Microbacterium trichothecenolyticum HY-17, a gut bacterium of the mole cricket Gryllotalpa orientalis. J Microbiol Biotechnol 24(7):943–953CrossRefGoogle Scholar
- Koecher KJ, Noack JA, Timm DA, Klosterbuer AS, Thomas W, Slavin JL (2014) Estimation and interpretation of fermentation in the gut: coupling results from a 24 h batch in vitro system with fecal measurements from a human intervention feeding study using fructooligosaccharides, inulin, gum acacia and pea fiber. J Agric Food Chem 62:1332–1337CrossRefGoogle Scholar
- McCormack UM, Curião T, Buzoianu SG, Prieto ML, Ryan T, Varley P, Crispie F, Magowan E, Metzler-Zebeli BU, Berry D, O’Sullivan O, Cotter PD, Gardiner GE, Lawlor PG (2017) Exploring a possible link between the intestinal microbiota and feed efficiency in pigs. Appl Environ Microbiol 83:e00380–e00317CrossRefGoogle Scholar
- Mirande C, Kadlecikova E, Matulova M, Capek P, Bernalier-Donadille A, Forano E, Béra-Maillet C (2010) Dietary fibre degradation and fermentation by two xylanolytic bacteria Bacteroides xylanisolvens XB1A and Roseburia intestinalis XB6B4 from the human intestine. J Appl Microbiol 109(2):451–460PubMedGoogle Scholar
- Park SH, Kim SA, Lee SI, Rubinelli PM, Roto SM, Pavlidis HO, McIntyre DR, Ricke SC (2017) Original XPCTM effect on Salmonella typhimurium and caecal microbiota from three different ages of broiler chickens when incubated in an anaerobic in vitro culture system. Front Microbiol 8:1070CrossRefGoogle Scholar
- Tarayre C, Brognaux A, Brasseur C, Bauwens J, Millet C, Mattéotti C, Destain J, Vandenbol M, Portetelle D, De Pauw E, Haubruge E, Francis F, Thonart P (2013) Isolation and cultivation of a xylanolytic Bacillus subtilis extracted from the gut of the termite Reticulitermes santonensis. Appl Biochem Biotechnol 171(1):225–245CrossRefGoogle Scholar