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3 Biotech

, 9:31 | Cite as

Phytochemical profile, anti-oxidant, anti-inflammatory, and anti-proliferative activities of Pogostemon deccanensis essential oils

  • Vinay KumarEmail author
  • Varsha Shriram
  • Rani Bhagat
  • Tushar Khare
  • Shivanjali Kapse
  • Narendra Kadoo
Original Article
  • 89 Downloads

Abstract

Essential oils (EOs) obtained from aerial parts of Pogostemon deccanensis were analyzed for GC–MS profiling, and evaluated for antioxidant, anti-inflammatory, and anti-proliferative activities. GC–MS analysis revealed a total of 47 constituents, establishing the EOs rich in sesquiterpene with > 20 sesquiterpenes constituting around 77% of the total EO yield. Major constituents included Curzerene (Benzofuran, 6-ethenyl-4,5,6,7-tetrahydro-3,6-dimethyl-5-isopropenyl-, trans-) (26.39%) and epi-Cadinol (22.68%), Ethanone, 1-(2,4,6-trihydroxyphenyl) (6.83%, Acetophenones), and Boldenone (3.47%, anabolic steroid). EOs found to be rich in phytochemicals attributed for antioxidant potentials of aromatic/medicinal plants, viz., flavonoids (2.71 µg quercetin equivalents g−1 EO), total phenols (3.94 µg gallic acid equivalents (GAE) g−1 EO), carotenoids (14.3 µg β-carotene equivalents g−1 EO), and ascorbic acid (2.21 µg ascorbic acid equivalents g−1 EO). P. deccanensis EOs exhibited striking antioxidant activities assessed by wide range of assays including ferric reducing antioxidant potential (FRAP, 255.3 GAE at 2 µg mL−1 EO), total antioxidant activity (TAA, 264.3 GAE at 2 µg ml−1) of EO, DPPH (65% inhibition at 2 µg mL−1), and OH (58% inhibition at 2 µg mL−1) scavenging. Interestingly, EOs showed considerably higher anti-lipid peroxidation activity than the standard antioxidant molecule ascorbic acid, with 50% protection by 1.29 µg mL−1 EO against 20.0 µg mL−1 standard. EOs showed strong anti-inflammatory activity with 50% inhibition at 1.95 µg mL−1 EO. The anti-proliferative activity of EOs was tested against mouse cancer cell line and the EOs proved a potent anti-proliferative agent with only 2.1% cell survival at 2 µg mL−1 EO, whereas the EOs were largely non-toxic-to-normal (non-cancerous) cells with approximately 80% cell survival at the 2 µg mL−1 EOs. This being the first attempt of phytochemical profiling and wide array of biological activities of P. deccanensis EOs holds significance as the striking activities were observed at very low concentrations, in some cases at lower than the commercial standards, and has, therefore, great potential for pharmaceutical or commercial exploration.

Keywords

Antioxidants Anti-inflammatory activity Anti-proliferative activity Cytotoxicity Essential oils 

Notes

Acknowledgements

The authors would like to acknowledge the use of facilities created under FIST program of Department of Science and Technology (DST), Government of India and Star Status from the Department of Biotechnology (DBT), Government of India to Modern College, Ganeshkhind, Pune.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest in the publication.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of BiotechnologyModern College of Arts, Science and Commerce (Savitribai Phule Pune University)PuneIndia
  2. 2.Department of Environmental ScienceSavitribai Phule Pune UniversityPuneIndia
  3. 3.Department of BotanyProf. Ramkrishna More Arts, Commerce and Science College (Savitribai Phule Pune University)PuneIndia
  4. 4.Department of BotanyBaburaoji Gholap College (Savitribai Phule Pune University)PuneIndia
  5. 5.Biochemical Sciences DivisionCSIR National Chemical LaboratoryPuneIndia

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