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3 Biotech

, 9:61 | Cite as

Isolation and characterization of an estrogen-degrading Pseudomonas putida strain SJTE-1

  • Pingping Wang
  • Daning Zheng
  • Rubing LiangEmail author
Original Article

Abstract

In this report, Pseudomonas putida SJTE1 isolated from an enrichment culture of sludge was confirmed to degrade natural estrogens (17β-estradiol, estrone, estriol), estrogenic chemicals (naphthalene and phenanthrene) and testosterone. The strain completely degraded 1 mg/L 17β-estradiol in 24 h and transformed it into estrone; 90% and 75% of 50 mg/L and 100 mg/L 17β-estradiol were utilized in 7 days, respectively. The transformation efficiency of this strain against natural estrogens was much higher than that against other estrogenic chemicals. Organic carbon sources, lipopolysaccharide and surfactants could enhance the degradation efficiency of strain SJTE-1 against 17β-estradiol. The adsorption of 17β-estradiol onto the biomass was the premise for transmembrane and cellular utilization of this chemical. This work has the potential to bioremediate the environmental estrogens.

Keywords

Environmental estrogens Biodegradation Pseudomonas putida SJTE-1 17β-Estradiol Estrone 

Notes

Author contributions

RL designed the experiments and wrote the manuscript. DZ and PW performed the experiments. XW assisted the experiments. All the authors discussed the results and commented on the manuscript.

Funding

This work was supported by the National Science Foundation of China (Grant no. 31370152, 31570099).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethics approval and consent to participate

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

13205_2018_1537_MOESM1_ESM.pdf (161 kb)
Supplementary material 1 (PDF 161 KB)
13205_2018_1537_MOESM2_ESM.pdf (155 kb)
Supplementary material 2 (PDF 154 KB)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Microbial Metabolism, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina

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