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3 Biotech

, 9:14 | Cite as

Antibacterial activity and action mechanism of questin from marine Aspergillus flavipes HN4-13 against aquatic pathogen Vibrio harveyi

  • Lei GuoEmail author
  • Fei Zhang
  • Xintong Wang
  • Hui Chen
  • Qianqian Wang
  • Jiacai Guo
  • Xi Cao
  • Le Wang
Original Article
  • 35 Downloads

Abstract

This study investigated the antibacterial activity and mechanism of questin from marine Aspergillus flavipes HN4-13 against aquatic pathogenic Vibrio harveyi. The minimal inhibitory concentration and minimal bactericidal concentration of questin against V. harveyi strain SZ-1 and 1.8690 were determined by Oxford cup and tube dilution methods. The mechanism of action of questin against V. harveyi 1.8690 was investigated by bacterial growth curve analysis, ultraviolet absorption, Mo-Sb-Vc colorimetry, alkaline phosphatase and scanning electron microscopy. Results showed that questin exhibited favourable antibacterial and bactericidal activity against V. harveyi by disrupting the cell wall and membrane, which caused the destruction of permeability and integrity of cell wall and membrane, resulting in the leakage of intracellular biological components and change of cell morphology. This paper is the first to report the mechanism of action of questin against the aquatic pathogen V. harveyi.

Keywords

Questin Vibrio harveyi Antibacterial activity Action mechanism 

Notes

Acknowledgements

This work financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (CXKT20180216), Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX18_2585), Natural Science Foundation of Jiangsu Province (BK20151283), Technical Plan Project of Lianyungang (CG1612) and 521 Talented Project of Lianyungang (KKC17001).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Cao H, He S, Wei R, Diong M, Lu L (2011) Bacillus amyloliquefaciens G1: a potential antagonistic bacterium against eel-pathogenic Aeromonas hydrophila. Evid Based Complement Alternat Med 2011:824104PubMedPubMedCentralGoogle Scholar
  2. Gomes NG, Lefranc F, Kijjoa A, Kiss R (2015) Can some marine-derived fungal metabolites become actual anticancer agents? Mar Drugs 13:3950–3991CrossRefGoogle Scholar
  3. Guo L, Wang C (2017) Optimized production and isolation of antibacterial agent from marine Aspergillus flavipes against Vibrio harveyi. 3 Biotech 7:383CrossRefGoogle Scholar
  4. Guo L, Wang C, Zhu W, Xu F (2016) Bioassay-guided fractionation and identification of active substances from the fungus Aspergillus tubingensis against Vibrio anguillarum. Biotechnol Biotec Eq 30:602–606CrossRefGoogle Scholar
  5. Guo L, Guo J, Xu F (2017) Optimized extraction process and identification of antibacterial substances from Rhubarb against aquatic pathogenic Vibrio harveyi. 3 Biotech 7:377CrossRefGoogle Scholar
  6. Harikrishnan R, Balasundaram C, Heo MS (2010) Effect of probiotics enriched diet on Paralichthys olivaceus infected with lymphocystis disease virus (LCDV). Fish Shellfish Immunol 29:868–874CrossRefGoogle Scholar
  7. Jin L, Quan C, Hou X, Fan S (2016) Potential pharmacological resources: natural bioactive compounds from marine-derived fungi. Mar Drugs 14:76CrossRefGoogle Scholar
  8. Kesarcodi-Watson A, Kaspar H, Lategan MJ, Gibson L (2008) Probiotics in aquaculture: the need, principles and mechanisms of action and screening processes. Aquaculture 274:1–14CrossRefGoogle Scholar
  9. Li DL, Li XM, Wang BG (2009) Natural anthraquinone derivatives from a marine mangrove plant-derived endophytic fungus Eurotium rubrum: structural elucidation and DPPH radical scavenging activity. J Microbiol Biotechnol 19:675–680PubMedGoogle Scholar
  10. Li X, He C, Song L, Li T, Cui S, Zhang L, Jia Y (2017) Antimicrobial activity and mechanism of Larch bark procyanidins against Staphylococcus aureus. Acta Biochim Biophys Sin 49:1058–1066CrossRefGoogle Scholar
  11. Lin T, Lu C, Shen Y (2009) Secondary metabolites of Aspergillus sp. F1, a commensal fungal strain of Trewia nudiflora. Nat Prod Res 23:77–85CrossRefGoogle Scholar
  12. Liu R, Zhu W, Zhang Y, Zhu T, Liu H, Fang Y, Gu Q (2006) A new diphenyl ether from marine-derived fungus Aspergillus sp. B-F-2. J Antibiot (Tokyo) 59:362–365CrossRefGoogle Scholar
  13. Lv Z, Zhang Q, Chen R, Yu D (2011) Alkaloids and anthraquinones from branches and leaves of Uvaria kurzii. Chin J Chin Mat Med 36:1190–1192Google Scholar
  14. Minahk CJ, Farías ME, Sesma F, Morero RD (2000) Effect of enterocin CRL35 on Listeria monocytogenes cell membrane. FEMS Microbiol Lett 192:79–83CrossRefGoogle Scholar
  15. Morya VK, Choi W, Kim EK (2014) Isolation and characterization of Pseudoalteromonas sp. from fermented Korean food, as an antagonist to Vibrio harveyi. Appl Microbiol Biotechnol 98:1389–1395CrossRefGoogle Scholar
  16. Park YB, Kim SB (2011) Isolation and identification of antitumor promoters from the seeds of Cassia tora. J Microbiol Biotechnol 21:1043–1048CrossRefGoogle Scholar
  17. Patra JK, Baek KH (2016) Antibacterial activity and action mechanism of the essential oil from Enteromorpha linza L. against foodborne pathogenic bacteria. Molecules 21:388CrossRefGoogle Scholar
  18. Qu L, She P, Wang Y, Liu F, Zhang D, Chen L, Luo Z, Xu H, Qi Y, Wu Y (2016) Effects of norspermidine on Pseudomonas aeruginosa biofilm formation and eradication. Microbiologyopen 5:402–412CrossRefGoogle Scholar
  19. Randrianarivelo R, Danthu P, Benoit C, Ruez P, Raherimandimby M, Sarter S (2010) Novel alternative to antibiotics in shrimp hatchery: effects of the essential oil of Cinnamosma fragrans on survival and bacterial concentration of Penaeus monodon larvae. J Appl Microbiol 109:642–650PubMedGoogle Scholar
  20. Reddy KV, Yedery RD, Aranha C (2004) Antimicrobial peptides: premises and promises. Int J Antimicrob Agents 24:536–547CrossRefGoogle Scholar
  21. Song F, Ren B, Chen C, Yu K, Liu X, Zhang Y, Yang N, He H, Liu X, Dai H, Zhang L (2014) Three new sterigmatocystin analogues from marine-derived fungus Aspergillus versicolor MF359. Appl Microbiol Biotechnol 98:3753–3758CrossRefGoogle Scholar
  22. Su Y, Zhang C, Wang Y, Li P (2012) Antibacterial property and mechanism of a novel Pu-erh tea nanofibrous membrane. Appl Microbiol Biotechnol 93:1663–1671CrossRefGoogle Scholar
  23. Sun K, Li Y, Guo L, Wang Y, Liu P, Zhu W (2014) Indole diterpenoids and isocoumarin from the fungus, Aspergillus flavus, isolated from the prawn. Penaeusvannamei Mar Drugs 12:3970–3981CrossRefGoogle Scholar
  24. Thompson J, Gregory S, Plummer S, Shields RJ, Rowley AF (2010) An in vitro and in vivo assessment of the potential of Vibrio spp. as probiotics for the Pacific white shrimp, Litopenaeus vannamei. J Appl Microbiol 109:1177–1187CrossRefGoogle Scholar
  25. Turker H, Yıldırım AB (2015) Screening for antibacterial activity of some Turkish plants against fish pathogens: a possible alternative in the treatment of bacterial infections. Biotechnol Biotec Eq 29:281–288CrossRefGoogle Scholar
  26. Wang C, Guo L, Hao J, Wang L, Zhu W (2016) α–Glucosidase inhibitors from the marine-derived fungus Aspergillus flavipes HN4-13. J Nat Prod 79:2977–2981CrossRefGoogle Scholar
  27. Xu HM, Rong YJ, Zhao MX, Song B, Chi ZM (2014) Antibacterial activity of the lipopetides produced by Bacillus amyloliquefaciens M1 against multidrug-resistant Vibrio spp. isolated from diseased marine animals. Appl Microbiol Biotechnol 98:127–136CrossRefGoogle Scholar
  28. Yu M, Wang J, Tang K, Shi X, Wang S, Zhu WM, Zhang XH (2012) Purification and characterization of antibacterial compounds of Pseudoalteromonas flavipulchra JG1. Microbiology 158:835–842CrossRefGoogle Scholar
  29. Yuan YY, Liu QM, Zhong YS, Liao FP, Lin JR (2012) Mechanism of CP7 antibacterial protein against Aeromonas hydrophila. Microbiology China 39:949–957Google Scholar
  30. Zhang L, Tian X, Kuang S, Liu G, Zhang C, Sun C (2017) Antagonistic activity and mode of action of phenazine-1-carboxylic acid, produced by marine bacterium Pseudomonas aeruginosa PA31x, against Vibrio anguillarum in vitro and in a zebrafish in vivo model. Front Microbiol 8:289PubMedPubMedCentralGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Lei Guo
    • 1
    • 2
    • 3
    Email author
  • Fei Zhang
    • 1
    • 2
  • Xintong Wang
    • 1
    • 2
  • Hui Chen
    • 2
  • Qianqian Wang
    • 2
  • Jiacai Guo
    • 2
  • Xi Cao
    • 2
  • Le Wang
    • 2
  1. 1.Jiangsu Key Laboratory of Marine Bioresources and EnvironmentHuaihai Institute of TechnologyLianyungangChina
  2. 2.Jiangsu Key Laboratory of Marine Biotechnology, Co-Innovation Center of Jiangsu Marine Bio-industry TechnologyHuaihai Institute of TechnologyLianyungangChina
  3. 3.Jiangsu Institute of Marine Resources DevelopmentLianyungangChina

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