3 Biotech

, 9:14 | Cite as

Antibacterial activity and action mechanism of questin from marine Aspergillus flavipes HN4-13 against aquatic pathogen Vibrio harveyi

  • Lei GuoEmail author
  • Fei Zhang
  • Xintong Wang
  • Hui Chen
  • Qianqian Wang
  • Jiacai Guo
  • Xi Cao
  • Le Wang
Original Article


This study investigated the antibacterial activity and mechanism of questin from marine Aspergillus flavipes HN4-13 against aquatic pathogenic Vibrio harveyi. The minimal inhibitory concentration and minimal bactericidal concentration of questin against V. harveyi strain SZ-1 and 1.8690 were determined by Oxford cup and tube dilution methods. The mechanism of action of questin against V. harveyi 1.8690 was investigated by bacterial growth curve analysis, ultraviolet absorption, Mo-Sb-Vc colorimetry, alkaline phosphatase and scanning electron microscopy. Results showed that questin exhibited favourable antibacterial and bactericidal activity against V. harveyi by disrupting the cell wall and membrane, which caused the destruction of permeability and integrity of cell wall and membrane, resulting in the leakage of intracellular biological components and change of cell morphology. This paper is the first to report the mechanism of action of questin against the aquatic pathogen V. harveyi.


Questin Vibrio harveyi Antibacterial activity Action mechanism 



This work financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (CXKT20180216), Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX18_2585), Natural Science Foundation of Jiangsu Province (BK20151283), Technical Plan Project of Lianyungang (CG1612) and 521 Talented Project of Lianyungang (KKC17001).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Lei Guo
    • 1
    • 2
    • 3
    Email author
  • Fei Zhang
    • 1
    • 2
  • Xintong Wang
    • 1
    • 2
  • Hui Chen
    • 2
  • Qianqian Wang
    • 2
  • Jiacai Guo
    • 2
  • Xi Cao
    • 2
  • Le Wang
    • 2
  1. 1.Jiangsu Key Laboratory of Marine Bioresources and EnvironmentHuaihai Institute of TechnologyLianyungangChina
  2. 2.Jiangsu Key Laboratory of Marine Biotechnology, Co-Innovation Center of Jiangsu Marine Bio-industry TechnologyHuaihai Institute of TechnologyLianyungangChina
  3. 3.Jiangsu Institute of Marine Resources DevelopmentLianyungangChina

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