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3 Biotech

, 8:457 | Cite as

Recombinant occlusion bodies of baculovirus as carriers of a non-structural protein of foot-and-mouth disease virus

  • Michay Diez
  • Myrian Trotta
  • Victoria Alfonso
  • Oscar Taboga
  • María Gabriela LópezEmail author
Short Reports
  • 94 Downloads

Abstract

Here, we developed a diagnostic ELISA for foot-and-mouth disease using recombinant occlusion bodies (rOBs) of baculovirus. We fused Δ3AB1–3, a polypeptide derived from non-structural proteins of foot-and-mouth disease virus, to polyhedrin (POLH), the major constituent of OBs, under polh promoter. To further assess the most convenient strategy to improve yields, we designed two recombinant baculoviruses, vPOLH and vPOLHE44G. These carried the sequence of the fusion protein POLH-Δ3AB1–3 with an additional copy in cis of polh or polhE44G, respectively, under p10 promoter. Our results show that both viruses expressed POLH-Δ3AB1–3, which was detected by western blot in purified rOBs with anti-POLH and anti-3AB1 antibodies. We also found that vPOLHE44G produced larger polyhedra and a significant increase of antigen yield (p < 0.01). Furthermore, the chimeric protein POLH-Δ3AB1–3 was recognized by sera from experimentally infected animals, showing that translational fusion to POLH does not alter the antigenicity of Δ3AB1–3. Finally, the rOBs were successfully used in an ELISA test to differentiate infected from vaccinated animals. Taken together, these results demonstrate the great potential of rOBs to develop diagnostic schemes adaptable to animal infectious diseases.

Keywords

Occlusion bodies Polyhedrin Baculovirus FMDV DIVA diagnostic 

Notes

Author contributions

MD performed experiments, analyzed the data and wrote the manuscript. MT analyzed the data. VA conceived and designed the experiments and analyzed the data. OT conceived and designed the experiments. MGL conceived and designed the experiments, and wrote the manuscript. All authors commented on the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest in the publication.

Supplementary material

13205_2018_1482_MOESM1_ESM.pdf (175 kb)
Supplementary material 1 (PDF 174 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Instituto de BiotecnologíaInstituto Nacional de Tecnología Agropecuaria (INTA)HurlinghamArgentina
  2. 2.Instituto de VirologíaINTAHurlinghamArgentina
  3. 3.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos AiresArgentina

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