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3 Biotech

, 8:443 | Cite as

Evaluation of probiotic Lactobacillus plantarum against foodborne pathogens and its fermentation potential in improving Lolium multiflorum silage quality

  • Srigopalram Srisesharam
  • Hyung Soo Park
  • Ilavenil Soundharrajan
  • Palaniselvam Kuppusamy
  • Da Hye Kim
  • Indira A. Jayraaj
  • Kyung Dong Lee
  • Ki Choon Choi
Original Article
  • 60 Downloads

Abstract

The objective of this study was to isolate the lactic acid bacteria from fermented silage sample and analyze their antibacterial activities, probiotic properties, and fermentation potential in silage. Eleven lactic acid bacteria (LAB) were selected based on distinct morphologies and preliminary studies. Cell-free supernatant (CFS) was then prepared from the selected strains for antibacterial analysis. L-30 strain and its CFS showed highest inhibition (> 10 mm) against tested foodborne pathogens as compared to other strains. Hereafter, the strain L-30 was named as KCC-30 and used for further studies. KCC-30 can survive in the harsh conditions of GIT such as low pH ( 2) and bile salt environment (oxgal) than standard L. plantarum KACC-91016 (pH 2: 27.2% vs 20.5%; oxgal: 72.3% vs 57.7%, both p < 0.05). In addition, KCC-30 exhibited strong auto-aggregation (68.3% vs 51.5%) and co-aggregation (33% vs 23.9%) properties. For silage experiment, KCC-30 treatment did not alter the nutrient profiles of silage. At the same time, KCC-30 treatment increased the lactic acid content of silage as compared to untreated silage (5.55 DM% vs 3.11 DM%). An increase of lactic acid content in the silage is due to higher lactic acid bacteria population in KCC-30 treated silage (15.33 × 107 CFU/g vs 7.66 × 107 CFU/g) than untreated silage (p < 0.05). Overall data suggested that KCC-30 exhibited strong probiotic potential and improved the quality of Lolium multiflorum silage by increasing the lactic acid level. Therefore, KCC-30 could be considered as potential strain to improve the fermentation quality of L. multiflorum silage.

Keywords

Lactic acid bacteria Low pH Bile salt Co-aggregation Auto-aggregation Silage fermentation 

Notes

Acknowledgements

This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project title: Development of Quality Improvement and Standardization Technique for Low Moisture Storage Forage, Project no. “PJ010916012017”) funded by Rural Development Administration, Republic of Korea. This study was also supported by 2014 Postdoctoral Fellowship Program of National Institute of Animal Science funded by Rural Development Administration, Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors have no conflicts of interest related to this study to disclose.

Supplementary material

13205_2018_1449_MOESM1_ESM.pdf (1 mb)
Supplementary material 1 (PDF 1046 KB)
13205_2018_1449_MOESM2_ESM.pdf (76 kb)
Supplementary material 2 (PDF 75 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Srigopalram Srisesharam
    • 1
  • Hyung Soo Park
    • 1
  • Ilavenil Soundharrajan
    • 1
  • Palaniselvam Kuppusamy
    • 1
  • Da Hye Kim
    • 2
  • Indira A. Jayraaj
    • 3
  • Kyung Dong Lee
    • 4
  • Ki Choon Choi
    • 1
  1. 1.Grassland and Forage Division, National Institute of Animal ScienceRural Development AdministrationCheonanRepublic of Korea
  2. 2.Center for Research on Environmental Disease, College of MedicineUniversity of KentuckyLexingtonUSA
  3. 3.Department of BiochemistryKongunadu Arts and Science College (Autonomous)CoimbatoreIndia
  4. 4.Department of Oriental Medicine MaterialsDongshin UniversityNajuRepublic of Korea

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