Optimization of the enzymatic hydrolysis of rice protein by different enzymes using the response surface methodology
- 56 Downloads
The optimization of the enzymatic hydrolysis of rice protein was determined using an experimental design tool. The semi-purified protease of Bacillus licheniformis LBA 46 and commercial protease Alcalase 2.4 L were used to produce rice hydrolysates using pH values ranging from 6 to 10 and enzyme concentrations varying from 50 to 150 U/mL. The optimized conditions were validated, and using the chosen conditions (pH 10 and 100 U/mL of protease), it was possible to confirm that the model was predictive for oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) responses. The experimental values for the ORAC and FRAP responses were 940 and 18.78 TE µmol/g for the rice protein hydrolysates prepared with LBA protease and 1001.94 and 19.31 TE µmol/g for the rice protein hydrolysates prepared with Alcalase 2.4 L. After optimization of the enzymatic hydrolysis conditions, the antioxidant activity values increased when compared to the values for the intact rice protein: 324.97 TE µmol/g (ORAC) and 6.14 TE µmol/g (FRAP). It was also observed that the LBA protease had an action similar to the commercial protease, showing its potential for application in protein hydrolysis.
KeywordsOptimization Contour curve Rice Antioxidant Protease
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Cao W, Zhang C, Ji H, Hao J (2012) Optimization of peptic hydrolysis parameters for the production of angiotensin I-convertingenzyme inhibitory hydrolysate from Aceteschinensis through Plackett–Burman and response surface methodological approaches. J Sci Food Agric 92:42–48CrossRefPubMedGoogle Scholar
- Castro RJS, Sato HH (2014a) Advantages of an acid protease from Aspergillus oryzae over commercial preparations for production of whey protein hydrolysates with antioxidant activities. J Food Process 3:58–65Google Scholar
- Castro RJS, Sato HH (2015) A response surface approach on optimization of hydrolysis parameters for the production of egg white protein hydrolysates with antioxidant activities. Biocatal Agric Biotechnol 4:1 55–62Google Scholar
- Castro RJS, Cason VG, Sato HH (2017) Binary mixture of proteases increases the antioxidant properties of white bean (Phaseolus vulgaris L.) protein-derived peptides obtained by enzymatic hydrolysis. Biocatal Agric Biotechnol 10:291–297Google Scholar
- Charney J, Tomarelli RM (1947) A colorimetric method for the determination the proteolytic activity of duodenal juice. J Biol Chem 170:23 501–505Google Scholar
- Contesini FJ (2014) Production, characterization and application of proteases from Bacillus sp. Ph.D. thesis, University of CampinasGoogle Scholar
- Korhonen H, Pihlanto A (2006) Bioactive peptides: production and functionality. Int Dairy J 16:9 945–960Google Scholar
- Solouk A, Solati-Hashjin M, Najarian S, Mirzadeh H, Seifalian AM (2011) Optimization of acrylic acid grafting onto POSS-PCU nanocomposite using response surface methodology. Iran Polym J 20:2 91–107Google Scholar