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3 Biotech

, 8:374 | Cite as

Extraction of lignin, structural characterization and bioconversion of sugarcane bagasse after ionic liquid assisted pretreatment

  • Koel Saha
  • Poulami Dwibedi
  • Ankita Ghosh
  • Jaya Sikder
  • Sudip Chakraborty
  • Stefano Curcio
Original Article
  • 117 Downloads

Abstract

The primary focus of this work was to recover lignin and investigate the structural changes in sugarcane bagasse after pretreatment with ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM]oAc). 90% lignin recovery was achieved while bagasse was treated with [EMIM]oAc at 140 °C, 120 min reaction time and 1:20 bagasse to the ionic liquid ratio (w/w). The impact of ionic liquid pretreatment on bagasse was confirmed by qualitative analysis of untreated and pretreated bagasse. Scanning electron microscopy analysis exhibited the porous and irregular structure of bagasse after pretreatment. X-ray powder diffraction analysis verified a decrease in crystallinity as a result of the pretreatment process by showing a 14.7% reduction of Crystallinity index after ionic liquid treatment. The efficacy of [EMIM]oAc on bagasse treatment was also examined by enzymatic hydrolysis which manifested an increase in reducing sugar yield as a result of pretreatment. Maximum yield of 54.3% reducing sugar was obtained after 72 h enzymatic hydrolysis of pretreated bagasse. Recovered lignin was analyzed qualitatively. 1D NMR spectroscopy of lignin revealed the presence of essential functional groups whereas 2D NMR spectroscopy showed the dominance of etherified syringyl unit. Further ionic liquid recovery and reuse were substantiated by Gel permeation chromatography analysis of lignin. Weight average molecular weight (Mw) of lignin extracted by fresh [EMIM]oAc was obtained as 1769 g/mol (in the previous study) while lignin recovered by recycled [EMIM]oAc showed almost equal Mw 1765 g/mol in this study. Thus, the current investigation corroborated satisfactory performance of [EMIM]oAc in lignocellulose processing which further enhanced enzymatic hydrolysis in the subsequent step.

Keywords

Sugarcane bagasse Pretreatment Ionic liquid recycle Lignin recovery Enzymatic hydrolysis 

Abbreviations

SCB

Sugarcane bagasse

[EMIM]oAc

1-Ethyl-3-methylimidazolium acetate

IL

Ionic liquid

RTIL

Room temperature ionic liquid

FTIR

Fourier transform infrared spectrophotometer

NMR

Nuclear magnetic resonance

GPC

Gel Permeation chromatography

TGA

Thermogravimetric analysis

NREL

National Renewable Energy Laboratory

Notes

Acknowledgements

The authors acknowledge Department of Biotechnology, Government of India for the Grant provided to carry out the research vide no. DBT/In-Bz/2013-16/06.

References

  1. Adsul MG, Singhvi MS, Gaikaiwari SA, Gokhale DV (2011) Development of biocatalysts for production of commodity chemicals from lignocellulosic biomass. Bioresour Technol 102:304–4312CrossRefGoogle Scholar
  2. Antunes FAF, Chandel AK, Milessi TSS, Santos JC, Rosa CA, da Silva SS (2014) Bioethanol production from sugarcane bagasse by a novel Brazilian pentose fermenting yeast Scheffersomyces shehatae UFMG-HM 52.2: evaluation of fermentation medium. Int J Chem Eng 180681:1–8CrossRefGoogle Scholar
  3. Aradhey A (2018) Approved by Landry T. India sugar annual 2018. GAIN report no: IN8047.USDA Foreign Agricultural ServiceGoogle Scholar
  4. Bahrani S, Raeissi S, Sarshar M (2015) Experimental investigation of ionic liquid pretreatment of sugarcane bagasse with 1,3-dimethylimadazolium dimethyl phosphate. Bioresour Technol 185:411–415CrossRefPubMedGoogle Scholar
  5. Balat M (2011) Production of bioethanol from lignocellulosic materials via the biochemical pathway: a review. Energy Convers Manag 52:858–875CrossRefGoogle Scholar
  6. Casas A, Oliet M, Alonso MV, Rodriguez F (2012) Dissolution of Pinusradiata and Eucalyptus globulus woods in ionic liquids under microwave radiation: Lignin regeneration and characterization. Sep Purif Technol 97:115–122CrossRefGoogle Scholar
  7. Chirayil CJ, Joy J, Mathew L, Mozetic M, Koetz J, Thomas S (2014) Isolation and characterization of cellulose nanofibrils from Helicteres isora plant. Ind Crop Prod 59:27–34CrossRefGoogle Scholar
  8. Cox BJ, Ekerdt JG (2013) Pretreatment of yellow pine in an acidic ionic liquid: extraction of hemicellulose and lignin to facilitate enzymatic digestion. Bioresour Technol 134:59–65CrossRefPubMedGoogle Scholar
  9. Dadi AP, Varanasi S, Schall CA (2006) Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step. Biotechnol Bioeng 95:904–910CrossRefPubMedGoogle Scholar
  10. Ede R, Brunow G (1992) Application of 2-dimensional homonuclear and heteronuclear correlation NMR-spectroscopy to wood lignin structure determination. J Org Chem 57:1477–1480CrossRefGoogle Scholar
  11. Fu D, Mazza G (2011) Aqueous ionic liquid pretreatment of straw. Bioresour Technol 102:7008–7011CrossRefPubMedGoogle Scholar
  12. Garcia A, Toledano A, Serrano L, Egus I, Gonzalez M, Marin F, Labidi J (2009) Characterization of lignins obtained by selective precipitation. Sep Purif Technol 68:193–198CrossRefGoogle Scholar
  13. Ghaffar SH, Fan M (2013) Structural analysis for lignin characteristics in biomass straw. Biomass Bioenergy 57:264–279CrossRefGoogle Scholar
  14. Hage RE, Brosse N, Chrusciel L, Sanchez C, Sannigrahi P, Ragauskas A (2009) Characterization of milled wood lignin and ethanol organosolv lignin from miscanthus. Polym Degrad Stab 94:1632–1638CrossRefGoogle Scholar
  15. Hoareau W, Trindade WG, Siegmund B, Castellan A, Frollini E (2004) Sugar cane bagasse and curaua lignins oxidized by chlorine dioxide and reacted with furfuryl alcohol: characterization and stability. Polym degrad Stab 86:567–576CrossRefGoogle Scholar
  16. Kim JY, Shin EJ, Eom IY, Won K, Kim YH, Choi D, Choi IG, Choi JW (2011) Structural features of lignin macromolecules extracted with ionic liquid from poplar wood. Bioresour Technol 102:9020–9025CrossRefPubMedGoogle Scholar
  17. Klemm D, Heublein B, Fink HP, Bohn A (2005) Cellulose: fascinating biopolymer and sustainable raw material. Angew Chem Int Ed 44:3358–3393CrossRefGoogle Scholar
  18. Lan W, Liu C-F, Sun R-C (2011) Fractionation of bagasse into cellulose, hemicelluloses, and lignin with ionic liquid treatment followed by alkaline extraction. J Agric Food Chem 59:8691–8701CrossRefPubMedGoogle Scholar
  19. Lee SH, Doherty TV, Linhardt RJ, Dordick JS (2009) Ionic liquid-mediated selective extraction of lignin from wood leading to enhanced enzymatic cellulose hydrolysis. Biotechnol Bioeng 102:1368–1376CrossRefPubMedGoogle Scholar
  20. Li Q, He YC, Xian MO, Jun G, Yang JM, Li LZ (2009) Improving enzymatic hydrolysis of wheat straw using ionic liquid 1-ethyl-3-methyl imidazolium diethyl phosphate pretreatment. Bioresour Technol 100:3570–3575CrossRefPubMedGoogle Scholar
  21. Lopes AMda, Jaio C, Rubik KG, Bogel-Lukasik DF, Duarte E, Andreaus LC, Bogel-Lukasik J R (2013) Pre-treatment of lignocellulosic biomass using ionic liquids: wheat straw fractionation. Bioresour Technol 142:198–208CrossRefGoogle Scholar
  22. Miller GL (1959) Use of dinitrosalicyclic acid reagent for determination of reducing sugar. Anal Chem 31:426–428CrossRefGoogle Scholar
  23. Moghaddam L, Zhang Z, Wellard RM, Bartley JP, O’Hara IM, Doherty WOS (2014) Characterisation of lignins isolated from sugarcane bagasse pretreated with acidified ethylene glycol and ionic liquids. Biomass Bioenergy 70:498–512CrossRefGoogle Scholar
  24. Moubarik A, Grimi N, Boussetta N, Pizzi A (2013) Isolation and characterization of lignin from Moroccan sugarcane bagasse: production of lignin–phenol–formaldehyde wood adhesive. Ind crop prod 45:296–302CrossRefGoogle Scholar
  25. Olivier-Bourbigou H, Magna L, Morvan D (2010) Ionic liquids and catalysis: recent progress from knowledge to applications. App Catal A 373:1–56CrossRefGoogle Scholar
  26. Park S, Baker JO, Himmel ME, Parilla PA, Johnson DK (2010) Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance. Biotechnol Biofuels 3:1–10CrossRefGoogle Scholar
  27. Pinkert A, Goeke DF, Marsh KN, Pang S (2011) Extracting wood lignin without dissolving or degrading cellulose: investigations on the use of food additive-derived ionic liquids. Green Chem 13:3124–3136CrossRefGoogle Scholar
  28. Pu Y, Jiang N, Ragauskas AJ (2007) Ionic liquid as a green solvent for lignin. J Wood Chem Technol 27:1:23–33CrossRefGoogle Scholar
  29. Qiu Z, Aita GM (2013) Pretreatment of energy cane bagasse with recycled ionic liquid for enzymatic hydrolysis. Bioresour Technol 129:532–537CrossRefPubMedGoogle Scholar
  30. Qiu Z, Aita GM, Walker MS (2012) Effect of ionic liquid pretreatment on the chemical composition, structure and enzymatic hydrolysis of energy cane bagasse. Bioresour Technol 117:251–256CrossRefPubMedGoogle Scholar
  31. Saha K, Dasgupta J, Chakraborty S, Antunes FAF, Sikder J, Curcio S, dos Santos JC, Arafat HA, da Silva SS (2017a) Optimization of lignin recovery from sugarcane bagasse using ionic liquid aided pretreatment. Cellulose.  https://doi.org/10.1007/s10570-017-1330-x CrossRefGoogle Scholar
  32. Saha K, Maheswari RU, Sikder J, Chakraborty S, da Silva SS, dos Santos JC (2017b) Membranes as a tool to support biorefineries: applications in enzymatic hydrolysis, fermentation and dehydration for bioethanol production. Renew Sustain Energy Rev 74:873–890CrossRefGoogle Scholar
  33. Sarkar N, Ghosh SK, Banarjee S, Aikat K (2012) Bioethanol production from agricultural wastes: an overview. Renew Energy 19:19–27CrossRefGoogle Scholar
  34. Segal L, Creely J, Martin A Jr, Conrad C (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29:786–794CrossRefGoogle Scholar
  35. Sidik DAB, Ngadi N, Amin NAS (2013) Optimization of lignin production from empty fruit bunch via liquefaction with ionic liquid. Bioresour Technol 135:690–696CrossRefPubMedGoogle Scholar
  36. Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2012) Determination of structural carbohydrates and lignin in biomass. Technical report NREL/TP-510-42618. Laboratory Analytical Procedure (LAP). National Renewable Energy Laboratory, Golden, CO, USAGoogle Scholar
  37. Solomon S (2014) Sugarcane agriculture and sugar industry in India: at a glance. Sugar Tech 16(2):113–124CrossRefGoogle Scholar
  38. Sun YC, Xu JK, Xu F, Sun RC (2013) Efficient separation and physico-chemical characterization of lignin from eucalyptus using ionic liquid—organic solvent and alkaline ethanol solvent. Ind Crop Prod 47:277–285CrossRefGoogle Scholar
  39. Swatloski RP, Spear SK, Holbrey JD, Rogers RD (2002) Dissolution of cellulose with ionic liquids. J Am Chem soc 124:4974–4975CrossRefPubMedGoogle Scholar
  40. Tan HT, Lee KT (2012) Understanding the impact of ionic liquid pretreatment on biomass and enzymatic hydrolysis. Chem Eng J 183:448–858CrossRefGoogle Scholar
  41. Tan SSY, MacFarlane DR, Upfal J, Edye LA, Doherty WOS, Patti AF, Pringle JM, Scott JL (2009) Extraction of lignin from lignocellulose at atmospheric pressure using alkylbenzene sulfonate ionic liquid. Green Chem 11;3:339–345CrossRefGoogle Scholar
  42. Trinh LTP, Lee YJ, Lee JW, Lee HJ (2015) Characterization of ionic liquid pretreatment and the bioconversion of pretreated mixed softwood biomass. Biomass Bioenergy 81:1–8CrossRefGoogle Scholar
  43. Tymchyshyn M, Xu C (2010) Liquefaction of bio-mass in hot-compressed water for the production of phenolic compounds. Bioresour Technol 101:2483–2490CrossRefPubMedGoogle Scholar
  44. Xu JK, Sun YC, Sun RC (2015) Synergistic effects of ionic liquid plus alkaline pretreatments on eucalyptus: lignin structure and cellulose hydrolysis. Process Biochem 50:6;955–965Google Scholar
  45. Yoon LW, Ngoh GC, Chua ASM (2011) Comparison of ionic liquid, acid and alkali pretreatments for sugarcane bagasse enzymatic saccharification. J Chem Technol Biotechnol 86:134–138Google Scholar
  46. Yoon LW, Ang TN, Ngoh GC, Chua ASM (2012) Regression analysis on ionic liquid pretreatment of sugarcane bagasse and assessment of structural changes. Biomass Bioenergy 36:160–169CrossRefGoogle Scholar
  47. Yuan TQ, You TT, Wang W, Xu F, Sun RC (2013) Synergistic benefits of ionic liquid and alkaline pretreatments of poplar wood. Part 2: characterization of lignin and hemicelluloses. Bioresour Technol 136:345–350CrossRefPubMedGoogle Scholar
  48. Zhang J, Wang Y, Zhang L, Zhang R, Liu G, Cheng G (2014) Understanding changes in cellulose crystalline structure of lignocellulosic biomass during ionic liquid pretreatment by XRD. Bioresour Technol 151:402–405CrossRefPubMedGoogle Scholar
  49. Zhang P, Dong SJ, Ma HH, Zhang BX, Wang YF, Hu XM (2015) Fractionation of corn stover into cellulose, hemicellulose and lignin using a series of ionic liquids. Ind Crop Prod 76:688–696CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemical EngineeringNational Institute of Technology DurgapurDurgapurIndia
  2. 2.Department of Informatics, Modeling, Electronics and Systems Engineering (DIMES)University of CalabriaRendeItaly

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